{"id":30063,"date":"2025-10-23T13:35:57","date_gmt":"2025-10-23T09:05:57","guid":{"rendered":"https:\/\/medpip.com\/mag\/?p=30063"},"modified":"2025-10-23T13:35:57","modified_gmt":"2025-10-23T09:05:57","slug":"cyclospora-cayetanensis-molecular-detection","status":"publish","type":"post","link":"https:\/\/medpip.com\/mag\/cyclospora-cayetanensis-molecular-detection\/","title":{"rendered":"\u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u062f\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u06af\u06cc\u0627\u0647\u06cc \u062a\u0627\u0632\u0647"},"content":{"rendered":"<div class=\"wpb-content-wrapper\">[vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 ( Cyclospora cayetanensis ) \u0627\u0646\u06af\u0644 \u062a\u06a9\u200c\u06cc\u0627\u062e\u062a\u0647\u200c\u0627\u06cc \u0627\u0632 \u06af\u0631\u0648\u0647 \u06a9\u0648\u06a9\u0633\u06cc\u062f\u06cc\u0627 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0627\u0639\u062b \u0628\u06cc\u0645\u0627\u0631\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u06cc\u0627\u0632\u06cc\u0633 (Cyclosporiasis) \u00a0\u062f\u0631 \u0627\u0646\u0633\u0627\u0646 \u0645\u06cc\u200c\u0634\u0648\u062f. \u0627\u06cc\u0646 \u0627\u0646\u06af\u0644 \u0628\u06cc\u0634\u062a\u0631 \u0627\u0632 \u0637\u0631\u06cc\u0642 \u0645\u0635\u0631\u0641 \u0622\u0628 \u0648 \u063a\u0630\u0627\u0647\u0627\u06cc \u0622\u0644\u0648\u062f\u0647 (\u0628\u0647\u200c\u0648\u06cc\u0698\u0647 \u0633\u0628\u0632\u06cc\u062c\u0627\u062a \u0648 \u0645\u06cc\u0648\u0647\u200c\u0647\u0627\u06cc \u062e\u0627\u0645 \u0634\u0633\u062a\u0647\u200c\u0646\u0634\u062f\u0647) \u0645\u0646\u062a\u0642\u0644 \u0645\u06cc\u200c\u0634\u0648\u062f. \u0639\u0641\u0648\u0646\u062a \u0646\u0627\u0634\u06cc \u0627\u0632 \u0622\u0646 \u0628\u06cc\u0634\u062a\u0631 \u062f\u0631 \u0645\u0646\u0627\u0637\u0642 \u06af\u0631\u0645\u0633\u06cc\u0631\u06cc \u0648 \u0646\u06cc\u0645\u0647\u200c\u06af\u0631\u0645\u0633\u06cc\u0631\u06cc \u0634\u0627\u06cc\u0639 \u0627\u0633\u062a \u0648 \u0645\u0639\u0645\u0648\u0644\u0627\u064b \u0628\u0627 \u0639\u0644\u0627\u0626\u0645\u06cc \u0645\u0627\u0646\u0646\u062f \u0627\u0633\u0647\u0627\u0644 \u0622\u0628\u06a9\u06cc\u060c \u0646\u0641\u062e\u060c \u062a\u0647\u0648\u0639\u060c \u062f\u0631\u062f \u0634\u06a9\u0645\u06cc \u0648 \u062e\u0633\u062a\u06af\u06cc \u0637\u0648\u0644\u0627\u0646\u06cc\u200c\u0645\u062f\u062a \u0647\u0645\u0631\u0627\u0647 \u0627\u0633\u062a. \u0686\u0631\u062e\u0647 \u0632\u0646\u062f\u06af\u06cc \u0627\u06cc\u0646 \u0627\u0646\u06af\u0644 \u062f\u0631 \u0631\u0648\u062f\u0647 \u0627\u0646\u0633\u0627\u0646 \u06a9\u0627\u0645\u0644 \u0645\u06cc\u200c\u0634\u0648\u062f \u0648 \u0627\u0648\u0648\u0633\u06cc\u062a\u200c\u0647\u0627\u06cc \u062f\u0641\u0639\u200c\u0634\u062f\u0647 \u0627\u0632 \u0628\u062f\u0646\u060c \u067e\u0633 \u0627\u0632 \u0686\u0646\u062f \u0631\u0648\u0632 \u062f\u0631 \u0645\u062d\u06cc\u0637 \u062e\u0627\u0631\u062c\u06cc \u0628\u0627\u0644\u063a \u0648 \u0622\u0644\u0648\u062f\u0647\u200c\u06a9\u0646\u0646\u062f\u0647 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f. \u062c\u0647\u062a \u062c\u0644\u0648\u06af\u06cc\u0631\u06cc \u0627\u0632 \u0627\u0646\u062a\u0634\u0627\u0631 \u0627\u06cc\u0646 \u0628\u06cc\u0645\u0627\u0631\u06cc\u060c \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0627\u06cc\u0646 \u0627\u0646\u06af\u0644 \u0627\u0632 \u0627\u0647\u0645\u06cc\u062a \u0628\u0627\u0644\u0627\u06cc\u06cc \u0628\u0631\u062e\u0648\u0631\u062f\u0627\u0631 \u0627\u0633\u062a. \u062f\u0631 \u0645\u0642\u0627\u0644\u0647 \u062d\u0627\u0636\u0631 \u0631\u0648\u0634 \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0631\u0627 \u0627\u0632 \u0637\u0631\u06cc\u0642 <span style=\"color: #0000ff;\"><sup>[1]<\/sup><\/span>Real time PCR\u00a0\u0645\u0648\u0631\u062f \u0628\u0631\u0631\u0633\u06cc \u0642\u0631\u0627\u0631 \u0645\u06cc\u200c\u062f\u0647\u06cc\u0645.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h2 style=\"text-align: justify;\"><a name=\"_Toc208648538\"><\/a><span style=\"font-size: 24pt;\">1.\u0631\u0648\u0634 \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u062f\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0627\u0632\u0647 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 Real time PCR<\/span><\/h2>\n[\/vc_column_text][vc_single_image image=&#8221;30075&#8243; img_size=&#8221;full&#8221; add_caption=&#8221;yes&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06cc\u0646 \u0631\u0648\u0634 \u062a\u062d\u0644\u06cc\u0644\u06cc \u0634\u0627\u0645\u0644 \u0645\u0631\u0627\u062d\u0644\u06cc \u0628\u0631\u0627\u06cc \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0627\u0648\u0648\u0633\u06cc\u0633\u062a\u200c\u0647\u0627\u06cc \u00a0Cyclospora cayetanensis \u0627\u0632 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0627\u0632\u0647 \u0645\u06cc\u200c\u0628\u0627\u0634\u062f. \u0645\u0631\u0627\u062d\u0644 \u0627\u0646\u062c\u0627\u0645 \u0622\u0646 \u0634\u0627\u0645\u0644 \u0634\u0633\u062a\u200c\u0648\u0634\u0648 \u0648 \u0633\u067e\u0633 \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0627\u0644\u06af\u0648\u06cc DNA \u0627\u0632 \u0645\u062d\u0644\u0648\u0644\u200c\u0647\u0627\u06cc \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0645\u062d\u0635\u0648\u0644 \u0627\u0633\u062a. \u0627\u06cc\u0646 \u0639\u0645\u0644\u06cc\u0627\u062a \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a \u0645\u0646\u062c\u0631 \u0628\u0647 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u062e\u0648\u0627\u0647\u062f \u0634\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><a name=\"_Toc208648540\"><\/a><span style=\"font-size: 18pt;\">1.1. \u062a\u062c\u0647\u06cc\u0632\u0627\u062a \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647\u06cc \u062c\u0647\u062a \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06af\u0627\u0647\u06cc \u0648\u062c\u0648\u062f \u0622\u0644\u0648\u062f\u06af\u06cc \u0628\u0627\u0639\u062b \u0627\u06cc\u062c\u0627\u062f \u0646\u062a\u0627\u06cc\u062c \u0645\u062b\u0628\u062a \u06a9\u0627\u0630\u0628 \u062e\u0648\u0627\u0647\u062f \u0634\u062f. \u0628\u0631\u0627\u06cc \u062c\u0644\u0648\u06af\u06cc\u0631\u06cc \u0627\u0632 \u0622\u0646 \u0644\u0627\u0632\u0645 \u0627\u0633\u062a \u062a\u0645\u0627\u0645\u06cc \u0645\u0631\u0627\u062d\u0644 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u00a0\u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0627\u0639\u0645 \u0627\u0632 \u0645\u0631\u0627\u062d\u0644 \u0634\u0633\u062a\u0634\u0648\u06cc \u0646\u0645\u0648\u0646\u0647\u200c\u200c\u0647\u0627\u060c \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u0648 \u00a0Real Time PCR \u062f\u0631 \u0628\u062e\u0634\u200c\u0647\u0627\u06cc\u06cc \u0627\u0646\u062c\u0627\u0645 \u0634\u0648\u0646\u062f \u06a9\u0647 \u0627\u0632 \u0647\u0645 \u062c\u062f\u0627 \u0628\u0627\u0634\u0646\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u00a0\u0628\u0631\u0627\u06cc \u06a9\u0627\u0647\u0634 \u0627\u062d\u062a\u0645\u0627\u0644 \u0622\u0644\u0648\u062f\u06af\u06cc\u060c \u062a\u0648\u0635\u06cc\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f \u0645\u0631\u0627\u062d\u0644 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u062f\u0631 \u0646\u0648\u0627\u062d\u06cc \u0632\u06cc\u0631 \u0627\u0646\u062c\u0627\u0645 \u06af\u06cc\u0631\u0646\u062f:<\/span><br \/>\n<span style=\"font-size: 14pt;\">\u0627\u0644\u0641) \u06cc\u06a9 \u0645\u06cc\u0632 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647\u06cc \u0628\u0631\u0627\u06cc \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0645\u062d\u0635\u0648\u0644<\/span><br \/>\n<span style=\"font-size: 14pt;\">\u0628) \u06cc\u06a9 \u0647\u0648\u062f \u0628\u0631\u0627\u06cc \u0627\u0646\u062c\u0627\u0645 \u0641\u0631\u0622\u06cc\u0646\u062f \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><span style=\"font-size: 18pt;\">2.1. \u0645\u0648\u0627\u062f \u0648 \u062a\u062c\u0647\u06cc\u0632\u0627\u062a \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06a9\u06cc\u0633\u0647\u200c\u0647\u0627\u06cc \u0641\u06cc\u0644\u062a\u0631\u062f\u0627\u0631 Interscience BagPage\u00ae+ \u0628\u0627 \u062d\u062c\u0645 \u06f4\u06f0\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u060c \u0628\u0633\u062a\u0647 \u06f5\u06f0\u06f0 \u0639\u062f\u062f\u06cc\u060c \u0634\u0645\u0627\u0631\u0647 \u06a9\u0627\u062a\u0627\u0644\u0648\u06af EW-36840-56 (ColeParmer)<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06af\u06cc\u0631\u0647\u200c\u0647\u0627\u06cc \u06a9\u06cc\u0633\u0647 Interscience\u060c \u0628\u0633\u062a\u0647 \u06f5\u06f0 \u0639\u062f\u062f\u06cc\u060c \u0634\u0645\u0627\u0631\u0647 \u06a9\u0627\u062a\u0627\u0644\u0648\u06af EW-36850-46 (Cole-Parmer)<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u067e\u06cc\u067e\u062a\u200c\u0647\u0627\u06cc \u0633\u0631\u0648\u0644\u0648\u0698\u06cc\u06a9 \u06cc\u06a9\u200c\u0628\u0627\u0631 \u0645\u0635\u0631\u0641\u060c \u06f5 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0648 \u06f2\u06f5 \u06cc\u0627 \u06f5\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u06cc\u0646\u06cc \u0628\u0631\u0627\u06cc \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u06a9\u06cc\u0633\u0647\u200c\u0647\u0627\u06cc \u0641\u06cc\u0644\u062a\u0631\u062f\u0627\u0631 \u062f\u0631 \u0647\u0646\u06af\u0627\u0645 \u0634\u0633\u062a\u200c\u0648\u0634\u0648 (\u0645\u0637\u0627\u0628\u0642 \u062a\u0635\u0627\u0648\u06cc\u0631 2 \u0648 3)<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0633\u062a\u06af\u0627\u0647 Stovall Belly Dancer \u06cc\u0627 \u0647\u0631 \u067e\u0644\u062a\u0641\u0631\u0645 \u0645\u0634\u0627\u0628\u0647 \u0628\u0627 \u062d\u0631\u06a9\u062a \u0686\u0631\u062e\u0634\u06cc \u0628\u0631\u0627\u06cc \u0627\u062e\u062a\u0644\u0627\u0637<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0633\u062a\u06af\u0627\u0647 \u0631\u0627\u06a9\u0631 \u0634\u06cc\u06a9\u0631 (Platform rocker)<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0645\u062e\u0631\u0648\u0637\u06cc \u06f1\u06f5 \u0648 \u06f5\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0628\u0631\u0627\u06cc \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0645\u062d\u0635\u0648\u0644<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06cc\u062e\u0686\u0627\u0644\u200c\u062f\u0627\u0631 Sorvall Legend RT+ \u06cc\u0627 \u0645\u0639\u0627\u062f\u0644 \u0622\u0646 (\u0628\u0631\u0627\u06cc \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0631\u062f\u0646 \u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0645\u062e\u0631\u0648\u0637\u06cc \u06f1\u06f5 \u0648 \u06f5\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc)<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0641\u0644\u0627\u0633\u06a9 \u062e\u0644\u0623 \u06f2 \u0644\u06cc\u062a\u0631\u06cc (\u06cc\u0627 \u0628\u0632\u0631\u06af\u200c\u062a\u0631) \u0645\u062a\u0635\u0644 \u0628\u0647 \u067e\u0645\u067e \u062e\u0644\u0623 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u067e\u06cc\u067e\u062a\u200c \u067e\u0627\u0633\u062a\u0648\u0631\u200c\u0647\u0627\u06cc \u0634\u06cc\u0634\u0647\u200c\u0627\u06cc \u06a9\u0648\u062a\u0627\u0647 \u0628\u0631\u0627\u06cc \u0628\u0631\u062f\u0627\u0634\u062a \u0645\u062d\u0644\u0648\u0644\u200c\u0647\u0627\u06cc \u0634\u0633\u062a\u200c\u0648\u0634\u0648 \u062a\u062d\u062a \u062e\u0644\u0623<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u062e\u0627\u0644\u06cc FastPrep\u00ae \u0628\u0627 \u062d\u062c\u0645 \u06f2 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0648 \u062f\u0631\u067e\u0648\u0634\u060c \u0634\u0645\u0627\u0631\u0647 \u06a9\u0627\u062a\u0627\u0644\u0648\u06af 115076400 \u0648 115064002 (MP Biomedicals)<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0645\u06cc\u06a9\u0631\u0648\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06f2.\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0641\u0627\u0642\u062f DNase<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0633\u062a\u06af\u0627\u0647 FastPrep\u00ae-24 (MP Biomedicals) \u06cc\u0627 \u0647\u0645\u0648\u0698\u0646\u0627\u06cc\u0632\u0631 \u0645\u0634\u0627\u0628\u0647<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0645\u062e\u0631\u0648\u0637\u06cc \u06f1\u06f5 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0628\u0631\u0627\u06cc \u0645\u0631\u062d\u0644\u0647 \u0627\u062a\u0635\u0627\u0644 DNA<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0631\u0648\u0645\u06cc\u0632\u06cc \u0645\u0646\u0627\u0633\u0628 \u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u06f2.\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u06cc\u06a9\u0631\u0648\u067e\u06cc\u067e\u062a\u200c\u0647\u0627<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u0631\u067e\u06cc\u067e\u062a\u200c\u0647\u0627\u06cc \u0645\u0642\u0627\u0648\u0645 \u062f\u0631 \u0628\u0631\u0627\u0628\u0631 \u0622\u0626\u0631\u0648\u0633\u0644(Aerosol)<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0633\u062a\u06a9\u0634 \u0644\u0627\u062a\u06a9\u0633 \u06cc\u0627 \u0646\u06cc\u062a\u0631\u06cc\u0644<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0633\u062a\u06af\u0627\u0647 \u0648\u0631\u062a\u06a9\u0633 \u0645\u06cc\u06a9\u0633\u0631<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><a name=\"_Toc208648542\"><\/a><span style=\"font-size: 18pt;\">3.1. \u0645\u0648\u0627\u062f \u0634\u06cc\u0645\u06cc\u0627\u06cc\u06cc (\u0648\u0627\u06a9\u0646\u0634\u200c\u06af\u0631\u0647\u0627) \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u0644\u0641) \u067e\u0648\u062f\u0631 \u0634\u0648\u06cc\u0646\u062f\u0647 \u0634\u06cc\u0634\u0647\u200c\u0622\u0644\u0627\u062a \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647\u06cc Alconox\u00ae\u060c \u0634\u0645\u0627\u0631\u0647 \u0642\u0637\u0639\u0647 \u00a0EW-17775-0 (ColeParmer)<\/span><br \/>\n<span style=\"font-size: 14pt;\">\u06f1. \u0645\u062d\u0644\u0648\u0644 \u0627\u0633\u062a\u0648\u06a9 \u06f1.\u06f0\u066a Alconox <\/span><br \/>\n<span style=\"font-size: 14pt;\">\u06f2. \u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0645\u062d\u0635\u0648\u0644 \u06f0.\u06f1\u066a Alconox<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628) \u06a9\u06cc\u062a FastDNA\u00ae SPIN \u0645\u062e\u0635\u0648\u0635 \u062e\u0627\u06a9\u060c \u0634\u0645\u0627\u0631\u0647 \u0642\u0637\u0639\u0647 6560-200 (MP Biomedicals)<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062c) \u0627\u062a\u0627\u0646\u0648\u0644 \u06f1\u06f0\u06f0\u066a \u0628\u0631\u0627\u06cc \u0641\u0631\u0622\u06cc\u0646\u062f \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f) \u0622\u0628 \u062f\u200c\u06cc\u0648\u0646\u06cc\u0632\u0647 \u0627\u0633\u062a\u0631\u06cc\u0644 \u0648 \u0639\u0627\u0631\u06cc \u0627\u0632 \u0646\u0648\u06a9\u0644\u0626\u0627\u0632 \u0628\u0631\u0627\u06cc \u0641\u0631\u0622\u06cc\u0646\u062f \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0645\u062d\u0635\u0648\u0644<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><a name=\"_Toc208648543\"><\/a><span style=\"font-size: 18pt;\">4.1. \u0631\u0648\u0634 \u0634\u0633\u062a\u0634\u0648 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0627\u0632\u0647 \u062c\u0647\u062a \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0627\u062f\u0627\u0645\u0647 \u0631\u0648\u0634 \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0627\u0632\u0647 \u062c\u0647\u062a \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u00a0\u062a\u0648\u0636\u06cc\u062d \u062f\u0627\u062f\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a. \u0627\u06cc\u0646 \u0631\u0648\u0634 \u0628\u0631\u0627\u06cc \u0633\u0628\u0632\u06cc\u062c\u0627\u062a \u0628\u0631\u06af\u200c\u062f\u0627\u0631 \u0648 \u06af\u06cc\u0627\u0647\u0627\u0646 \u0645\u0639\u0637\u0631 \u06cc\u0627 \u0633\u0628\u0632\u06cc\u062c\u0627\u062a \u0645\u0642\u0627\u0648\u0645 \u0645\u0646\u0627\u0633\u0628 \u0627\u0633\u062a. \u0644\u0627\u0632\u0645 \u0627\u0633\u062a \u062a\u063a\u06cc\u06cc\u0631\u0627\u062a\u06cc \u06a9\u0647 \u062f\u0631 \u067e\u0631\u0648\u062a\u06a9\u0644 \u0628\u0631\u0627\u06cc \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u062d\u0633\u0627\u0633 (\u0645\u062b\u0644 \u062a\u0645\u0634\u06a9\u200c\u0647\u0627) \u0630\u06a9\u0631 \u0634\u062f\u0647 \u0631\u0639\u0627\u06cc\u062a \u0634\u0648\u062f. \u0632\u06cc\u0631\u0627 \u062f\u0631 \u0635\u0648\u0631\u062a \u0639\u062f\u0645 \u062f\u0642\u062a\u060c \u0627\u06cc\u0646 \u0646\u0648\u0639 \u0645\u06cc\u0648\u0647\u200c\u0647\u0627 \u0645\u0642\u062f\u0627\u0631 \u0632\u06cc\u0627\u062f\u06cc \u0630\u0631\u0627\u062a \u0632\u0627\u0626\u062f \u06cc\u0627 \u067e\u06a9\u062a\u06cc\u0646 \u0622\u0632\u0627\u062f \u0645\u06cc\u200c\u06a9\u0646\u0646\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u06a9\u062a\u0647: \u00a0\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0631\u062f\u0646 \u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a\u200c\u0648\u0634\u0648 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0637\u0628\u0642 \u062f\u0633\u062a\u0648\u0631 \u0632\u06cc\u0631 \u0648 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0631\u0648\u062a\u0648\u0631\u0647\u0627\u06cc \u0632\u0627\u0648\u06cc\u0647 \u0645\u062a\u063a\u06cc\u0631 \u06cc\u0627 \u0633\u0648\u06cc\u06cc\u0646\u06af \u00a0(swinging-bucket rotor) \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc\u200c\u0634\u0648\u062f. \u0633\u0631\u0639\u062a \u062a\u0631\u0645\u0632 \u062f\u0633\u062a\u06af\u0627\u0647 \u0628\u0627\u06cc\u062f \u0631\u0648\u06cc \u0639\u062f\u062f \u06f6 (\u0627\u0632 \u0645\u0642\u06cc\u0627\u0633 \u06f0 \u062a\u0627 \u06f9) \u062a\u0646\u0638\u06cc\u0645 \u0634\u0648\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>1.4.1.\u00a0 \u0637\u0631\u0632 \u062a\u0647\u06cc\u0647 \u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a\u0634\u0648\u06cc \u0645\u06cc\u0648\u0647 \u0648 \u0633\u0628\u0632\u06cc\u062c\u0627\u062a Alconox\u00ae<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]<span style=\"font-size: 14pt;\">\u06f1. \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0645\u062d\u0644\u0648\u0644 \u0630\u062e\u06cc\u0631\u0647 \u06f1\u066a Alconox : \u0645\u06cc\u0631\u0627\u0646 10 \u06af\u0631\u0645 Alconox \u0631\u0627 \u062f\u0631 \u06f1 \u0644\u06cc\u062a\u0631 \u0622\u0628 \u0645\u0642\u0637\u0631 \u062d\u0644 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06f2. \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a\u0634\u0648 \u06f0.\u06f1\u066a Alconox : \u0645\u0642\u062f\u0627\u0631 200 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0627\u0632 \u0645\u062d\u0644\u0648\u0644 \u0630\u062e\u06cc\u0631\u0647 \u06f1\u066a \u0631\u0627 \u0628\u0627 \u06f1\u06f8\u06f0\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0622\u0628 \u0645\u0642\u0637\u0631 \u0645\u062e\u0644\u0648\u0637 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>2.4.1. \u0648\u0632\u0646 \u06a9\u0631\u062f\u0646 \u0646\u0645\u0648\u0646\u0647 \u0645\u0648\u0631\u062f \u0628\u0631\u0631\u0633\u06cc<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u0642\u062f\u0627\u0631 \u0645\u062d\u0635\u0648\u0644\u06cc \u06a9\u0647 \u0628\u0627\u06cc\u062f \u062c\u0647\u062a \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633\u060c \u0622\u0646\u0627\u0644\u06cc\u0632 \u0634\u0648\u062f \u0631\u0627 \u062f\u0627\u062e\u0644 \u06a9\u06cc\u0633\u0647 \u0641\u06cc\u0644\u062a\u0631\u062f\u0627\u0631 \u00a0BagPage\u00ae+ \u0648\u0632\u0646 \u06a9\u0646\u06cc\u062f (\u06f2\u06f5 \u06af\u0631\u0645 \u0645\u062d\u0635\u0648\u0644 \u062a\u0627\u0632\u0647 \u06cc\u0627 \u06f5\u06f0 \u06af\u0631\u0645 \u062a\u0648\u062a \u062a\u0627\u0632\u0647).<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>3.4.1. \u0627\u0641\u0632\u0648\u062f\u0646 \u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a\u200c\u0648\u0634\u0648 \u0648 \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u06a9\u06cc\u0633\u0647<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u00a0\u0628\u0647 \u0646\u0645\u0648\u0646\u0647 \u0645\u062d\u0635\u0648\u0644 \u062f\u0631\u0648\u0646 \u06a9\u06cc\u0633\u0647 \u0641\u06cc\u0644\u062a\u0631\u062f\u0627\u0631\u060c \u06f1\u06f0\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0645\u062d\u0644\u0648\u0644 \u06f0.\u06f1\u066a \u00a0Alconox\u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f. \u0628\u062e\u0634 \u067e\u0627\u06cc\u06cc\u0646\u06cc \u06a9\u06cc\u0633\u0647 \u0631\u0627 \u0635\u0627\u0641 \u0631\u0648\u06cc \u0645\u06cc\u0632 \u0642\u0631\u0627\u0631 \u062f\u0647\u06cc\u062f \u0648 \u0644\u0628\u0647\u200c\u06cc \u0628\u0627\u0632 \u06a9\u06cc\u0633\u0647 \u0631\u0627 \u0628\u0647 \u0633\u0645\u062a \u06cc\u06a9 \u062a\u06a9\u06cc\u0647\u200c\u06af\u0627\u0647 \u0639\u0645\u0648\u062f\u06cc \u062a\u0627 \u06a9\u0646\u06cc\u062f (\u062a\u0635\u0648\u06cc\u0631 2).<\/span><\/p>\n[\/vc_column_text][vc_single_image image=&#8221;30079&#8243; img_size=&#8221;full&#8221; add_caption=&#8221;yes&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06a9\u06cc\u0633\u0647\u200c\u0647\u0627\u06cc\u06cc \u06a9\u0647 \u062d\u0627\u0648\u06cc \u0633\u0628\u0632\u06cc\u062c\u0627\u062a \u0628\u0631\u06af\u200c\u062f\u0627\u0631 \u06cc\u0627 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\u0647\u0648\u0627 \u0628\u0633\u062a\u0647 \u0634\u0648\u0646\u062f. \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a\u060c \u062f\u0631\u0628 \u06a9\u06cc\u0633\u0647\u200c\u0647\u0627 \u0628\u0627 \u06af\u06cc\u0631\u0647 \u0628\u0633\u062a\u0647 \u0634\u0648\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>4.4.1. \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u06a9\u06cc\u0633\u0647\u200c\u0647\u0627<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06a9\u06cc\u0633\u0647\u200c\u0647\u0627\u06cc \u0628\u0633\u062a\u0647\u200c\u0634\u062f\u0647 \u062d\u0627\u0648\u06cc \u0633\u0628\u0632\u06cc\u062c\u0627\u062a \u0628\u0631\u06af\u200c\u062f\u0627\u0631 \u00a0\u062c\u0647\u062a \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u060c \u0631\u0627 \u0628\u0647 \u0635\u0648\u0631\u062a \u062a\u062e\u062a \u062f\u0627\u062e\u0644 \u06cc\u06a9 \u0633\u06cc\u0646\u06cc \u0631\u0648\u06cc \u0631\u0627\u06a9\u0631 \u0634\u06cc\u06a9\u0631 \u00a0(Rocking platform) \u0642\u0631\u0627\u0631 \u062f\u0647\u06cc\u062f. \u0644\u0628\u0647\u200c\u0647\u0627\u06cc \u0628\u0633\u062a\u0647\u200c\u0634\u062f\u0647 \u0622\u0646\u200c\u0647\u0627 \u0631\u0627 \u0628\u0647 \u062f\u06cc\u0648\u0627\u0631\u0647\u200c\u0647\u0627\u06cc \u0633\u06cc\u0646\u06cc \u062a\u06a9\u06cc\u0647 \u062f\u0647\u06cc\u062f \u062a\u0627 \u0627\u0632 \u0646\u0634\u062a \u0627\u062d\u062a\u0645\u0627\u0644\u06cc \u062c\u0644\u0648\u06af\u06cc\u0631\u06cc \u0634\u0648\u062f (\u062a\u0635\u0648\u06cc\u0631 3) .\u06a9\u06cc\u0633\u0647\u200c\u0647\u0627 \u0631\u0648\u06cc \u0647\u0645 \u0686\u06cc\u062f\u0647 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f \u062a\u0627 \u0647\u0645\u06af\u06cc \u062c\u0627 \u0634\u0648\u0646\u062f. \u0633\u067e\u0633 \u062f\u0631 \u062f\u0645\u0627\u06cc \u0627\u062a\u0627\u0642 \u0628\u0647 \u0645\u062f\u062a \u06f3\u06f0 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06f8\u06f5 \u062f\u0648\u0631 \u062f\u0631 \u062f\u0642\u06cc\u0642\u0647 (\u0645\u0639\u0627\u062f\u0644 \u062a\u0646\u0638\u06cc\u0645 \u062f\u0633\u062a\u06af\u0627\u0647 Stovall Belly Dancer \u00a0\u0631\u0648\u06cc \u06f7.\u06f0 \u0628\u0627 \u062d\u062f\u0627\u06a9\u062b\u0631 \u0634\u06cc\u0628) \u062a\u06a9\u0627\u0646 \u062f\u0627\u062f\u0647 \u0634\u0648\u0646\u062f. \u067e\u0633 \u0627\u0632 \u06f1\u06f5 \u062f\u0642\u06cc\u0642\u0647\u060c \u06a9\u06cc\u0633\u0647\u200c\u0647\u0627 \u0628\u0631\u06af\u0631\u062f\u0627\u0646\u062f\u0647 \u0634\u0648\u0646\u062f.<\/span><\/p>\n[\/vc_column_text][vc_single_image image=&#8221;30080&#8243; img_size=&#8221;full&#8221; add_caption=&#8221;yes&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06a9\u06cc\u0633\u0647\u200c\u0647\u0627\u06cc \u062d\u0627\u0648\u06cc \u062a\u0648\u062a \u0628\u0627\u06cc\u062f \u0628\u0647 \u0635\u0648\u0631\u062a \u0627\u06cc\u0633\u062a\u0627\u062f\u0647 \u062f\u0631 \u0633\u06cc\u0646\u06cc \u0642\u0631\u0627\u0631 \u062f\u0627\u062f\u0647 \u0634\u0648\u0646\u062f \u00a0\u062a\u0627 \u062a\u0645\u0627\u0633 \u0628\u0647\u062a\u0631 \u0628\u06cc\u0646 \u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a\u200c\u0648\u0634\u0648 \u0648 \u0628\u0627\u0641\u062a \u0645\u06cc\u0648\u0647 \u0627\u06cc\u062c\u0627\u062f \u0634\u0648\u062f (\u062a\u0635\u0648\u06cc\u0631 4). \u0633\u067e\u0633 \u0628\u0647 \u0645\u062f\u062a \u06f3\u06f0 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06a9\u0645 \u0628\u0647 \u0622\u0631\u0627\u0645\u06cc \u062a\u06a9\u0627\u0646 \u062f\u0627\u062f\u0647 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(\u0645\u062b\u0644\u0627\u064b \u06f1\u06f2 \u062d\u0631\u06a9\u062a \u062f\u0631 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0627 \u00a0Hoefer Red Rocker \u0631\u0648\u06cc \u06f5.\u06f0)<\/span><\/p>\n[\/vc_column_text][vc_single_image image=&#8221;30081&#8243; img_size=&#8221;full&#8221; add_caption=&#8221;yes&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>5.4.1. \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u00a0\u06a9\u06cc\u0633\u0647\u200c\u0647\u0627 \u0631\u0627 \u0628\u0627\u0632 \u06a9\u0631\u062f\u0647 \u0648 \u0645\u0627\u06cc\u0639 \u0631\u0648\u06cc\u06cc \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a (Supernatant) \u0631\u0627 \u0627\u0632 \u0633\u0645\u062a \u0641\u06cc\u0644\u062a\u0631 \u06a9\u06cc\u0633\u0647\u200c\u0647\u0627\u06cc BagPage\u00ae+\u0628\u0647 \u062f\u0648 \u0644\u0648\u0644\u0647 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0645\u062e\u0631\u0648\u0637\u06cc \u06f5\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0628\u0631\u0686\u0633\u0628\u200c\u06af\u0630\u0627\u0631\u06cc\u200c\u0634\u062f\u0647 \u0645\u0646\u062a\u0642\u0644 \u06a9\u0646\u06cc\u062f. \u0628\u0631\u0627\u06cc \u0627\u0646\u062a\u0642\u0627\u0644 \u0627\u0632 \u067e\u06cc\u067e\u062a \u0633\u0631\u0648\u0644\u0648\u0698\u06cc\u06a9 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>6.4.1. \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0631\u062f\u0646 \u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a\u200c\u0648\u0634\u0648<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0630\u0631\u0627\u062a \u062d\u0627\u0648\u06cc \u0627\u0648\u0648\u0633\u06cc\u062a\u200c\u0647\u0627 \u0631\u0627 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0631\u0648\u062a\u0648\u0631\u0647\u0627\u06cc \u0632\u0627\u0648\u06cc\u0647 \u0645\u062a\u063a\u06cc\u0631 \u06cc\u0627 \u0633\u0648\u06cc\u06cc\u0646\u06af\u060c \u0628\u0647 \u0645\u062f\u062a \u06f2\u06f0 \u062f\u0642\u06cc\u0642\u0647 \u062f\u0631 \u0633\u0631\u0639\u062a \u06f2,\u06f0\u06f0\u06f0 g \u00d7 \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u06a9\u0646\u06cc\u062f \u0648 \u0627\u0632 \u062a\u0646\u0638\u06cc\u0645 \u062a\u0631\u0645\u0632 \u0631\u0648\u06cc 6 (\u062f\u0631 \u0645\u0642\u06cc\u0627\u0633 0 \u062a\u0627 9) \u0628\u0631\u0627\u06cc 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\u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u062e\u0627\u0644\u06cc \u0631\u0627 \u0628\u0627 \u06f0.\u06f4 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0622\u0628 \u062f\u06cc\u0648\u0646\u06cc\u0632\u0647 \u0634\u0633\u062a\u200c\u0648\u0634\u0648 \u062f\u0627\u062f\u0647 \u0648 \u0627\u06cc\u0646 \u0645\u0627\u06cc\u0639 \u0631\u0627 \u0628\u0647 \u0644\u0648\u0644\u0647 \u06f2 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06af\u0631 \u062d\u062c\u0645 \u06a9\u0644 \u0631\u0633\u0648\u0628 \u0645\u0639\u0644\u0642 \u0648 \u0645\u0627\u06cc\u0639 \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0644\u0648\u0644\u0647 \u0628\u06cc\u0634 \u0627\u0632 \u0638\u0631\u0641\u06cc\u062a \u0644\u0648\u0644\u0647 \u06f2 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0628\u0648\u062f\u060c \u0628\u062e\u0634\u06cc \u0627\u0632 \u0646\u0645\u0648\u0646\u0647 \u0631\u0627 \u062f\u0631 \u0647\u0645\u0627\u0646 \u0644\u0648\u0644\u0647 \u00a0FastPrep \u0628\u0627 \u0633\u0631\u0639\u062a \u06f1\u06f4,\u06f0\u06f0\u06f0 g \u00d7 \u0628\u0647 \u0645\u062f\u062a \u06f4 \u062f\u0642\u06cc\u0642\u0647 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a \u0631\u0627 \u0628\u0647\u200c\u06af\u0648\u0646\u0647\u200c\u0627\u06cc \u062e\u0627\u0631\u062c \u06a9\u0646\u06cc\u062f \u06a9\u0647 \u0631\u0633\u0648\u0628 \u0645\u0639\u0644\u0642 \u062f\u0631 \u062a\u0647 \u0644\u0648\u0644\u0647 \u0628\u0647 \u0647\u0645 \u0646\u062e\u0648\u0631\u062f \u0648 \u0645\u0627\u0628\u0642\u06cc \u0631\u0633\u0648\u0628 \u0648 \u0645\u0627\u06cc\u0639 \u0634\u0633\u062a\u200c\u0648\u0634\u0648 \u0631\u0627 \u062f\u0648\u0628\u0627\u0631\u0647 \u0628\u0647 \u0647\u0645\u0627\u0646 \u0644\u0648\u0644\u0647 \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>11.4.1. \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0646\u0647\u0627\u06cc\u06cc \u0644\u0648\u0644\u0647 2 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u00a0\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u06f2 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc FastPrep \u06a9\u0647 \u062d\u0627\u0648\u06cc \u0628\u0642\u0627\u06cc\u0627\u06cc \u0634\u0633\u062a\u200c\u0648\u0634\u0648 \u0627\u0632 \u0645\u0631\u062d\u0644\u0647 10-4-1 \u0647\u0633\u062a\u0646\u062f \u0631\u0627 \u0628\u0627 \u0633\u0631\u0639\u062a \u06f1\u06f4,\u06f0\u06f0\u06f0 g \u00d7 \u00a0\u0628\u0647 \u0645\u062f\u062a \u06f4 \u062f\u0642\u06cc\u0642\u0647 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u062a\u0642\u0631\u06cc\u0628\u0627\u064b 100\u2013200 \u00b5L \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a \u0631\u0627 \u062f\u0648\u0631 \u0628\u0631\u06cc\u0632\u06cc\u062f \u0628\u062f\u0648\u0646 \u0627\u06cc\u0646\u06a9\u0647 \u0631\u0633\u0648\u0628 \u062c\u0627\u0628\u062c\u0627 \u0634\u0648\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u06a9\u062a\u0647: \u0627\u06af\u0631 \u062d\u062c\u0645 \u0646\u0645\u0648\u0646\u0647 \u0631\u0633\u0648\u0628 \u0645\u0639\u0644\u0642\u200c\u0634\u062f\u0647 \u0628\u06cc\u0634 \u0627\u0632 \u062d\u062f\u0648\u062f \u06f8\u06f5\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0628\u0627\u0634\u062f\u060c \u0628\u0627\u06cc\u062f \u0646\u0645\u0648\u0646\u0647 \u0628\u0647 \u062f\u0648 \u0644\u0648\u0644\u0647 \u06f2 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc FastPrep \u00a0\u062a\u0642\u0633\u06cc\u0645 \u0634\u0648\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>12.4.1. \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u06cc\u0627 \u0627\u062f\u0627\u0645\u0647 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u00a0\u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627 \u0631\u0627 \u0645\u06cc\u200c\u062a\u0648\u0627\u0646 \u06cc\u06a9 \u0634\u0628 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f4 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u06a9\u0631\u062f \u06cc\u0627 \u0628\u0644\u0627\u0641\u0627\u0635\u0644\u0647 \u0637\u0628\u0642 \u062a\u0648\u0636\u06cc\u062d\u0627\u062a \u0628\u062e\u0634 \u0628\u0639\u062f\u06cc \u062c\u0647\u062a \u062c\u062f\u0627\u0633\u0627\u0632\u06cc DNA \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u0645\u0648\u0631\u062f \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0642\u0631\u0627\u0631 \u062f\u0627\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h2 style=\"text-align: justify;\"><a name=\"_Toc208648546\"><\/a><span style=\"font-size: 24pt;\">2. \u062c\u062f\u0627\u0633\u0627\u0632\u06cc DNA \u0627\u0632 \u0631\u0633\u0648\u0628 \u0630\u0631\u0627\u062a \u0628\u0627\u0642\u06cc \u0645\u0627\u0646\u062f\u0647 \u0628\u0639\u062f \u0627\u0632 \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0627\u0632\u0647 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u06a9\u06cc\u062a FastDNA\u00ae SPIN \u00a0\u0645\u062e\u0635\u0648\u0635 \u062e\u0627\u06a9<\/span><\/h2>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u0627\u0632 \u0645\u062d\u0644\u0648\u0644\u200c\u0647\u0627\u06cc \u0634\u0633\u062a\u200c\u0648\u0634\u0648\u06cc \u0645\u062d\u0635\u0648\u0644 \u0632\u06cc\u0631 \u0647\u0648\u062f \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647\u06cc \u0627\u0646\u062c\u0627\u0645 \u0645\u06cc\u200c\u0634\u0648\u062f . \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u06a9\u06cc\u062a FastDNA SPIN \u0645\u062e\u0635\u0648\u0635 \u062e\u0627\u06a9 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u00a0\u0627\u0646\u062c\u0627\u0645 \u062e\u0648\u0627\u0647\u062f \u0634\u062f. \u00a0\u0639\u0645\u0644\u06cc\u0627\u062a \u0641\u0648\u0642 \u0637\u0628\u0642 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0627\u0635\u0644\u0627\u062d\u200c\u0634\u062f\u0647 \u0635\u0648\u0631\u062a \u0645\u06cc\u200c\u06af\u06cc\u0631\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">1.2. \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0645\u0648\u0627\u062f \u0642\u0628\u0644 \u0627\u0632 \u0634\u0631\u0648\u0639 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u0641\u0632\u0648\u062f\u0646 \u06f1\u06f0\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0627\u062a\u0627\u0646\u0648\u0644 \u06f1\u06f0\u06f0\u066a \u0628\u0647 \u0628\u0637\u0631\u06cc SWES-M \u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a\u200c\u0648\u0634\u0648*<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc Lysing Matrix E \u062d\u0627\u0648\u06cc \u062f\u0627\u0646\u0647\u200c\u0647\u0627*<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0645\u06cc\u06a9\u0631\u0648\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06f2 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0641\u0627\u0644\u06a9\u0648\u0646 \u06f1\u06f5 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u062d\u0627\u0648\u06cc \u06f1 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 Binding Matrix \u0645\u0639\u0644\u0642\u200c\u0634\u062f\u0647*<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0641\u06cc\u0644\u062a\u0631\u0647\u0627\u06cc \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 (Spin Filters) \u062f\u0631\u0648\u0646 \u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc (Catch tubes)*<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06cc\u06a9 \u0633\u0631\u06cc \u062f\u06cc\u06af\u0631 \u0627\u0632 \u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc (Catch tubes)*<\/span><\/li>\n<\/ul>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">*\u0627\u06cc\u0646 \u0645\u0648\u0627\u0631\u062f \u062f\u0631 \u062f\u0627\u062e\u0644 \u06a9\u06cc\u062a FastDNA\u00ae SPIN \u0645\u062e\u0635\u0648\u0635 \u062e\u0627\u06a9 \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u0646\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062d\u0627\u0644 \u0628\u0627 \u062f\u0631 \u062f\u0633\u062a \u062f\u0627\u0634\u062a\u0646 \u0645\u0648\u0627\u062f \u0645\u0648\u0631\u062f \u0646\u06cc\u0627\u0632 \u0637\u0628\u0642 \u067e\u0631\u0648\u062a\u06a9\u0644 \u0627\u0635\u0644\u0627\u062d \u200c\u0634\u062f\u0647 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u0628\u0627 FastDNA SPIN \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u00a0\u06a9\u0647 \u062f\u0631 \u0632\u06cc\u0631 \u0645\u0631\u062d\u0644\u0647 \u0628\u0647 \u0645\u0631\u062d\u0644\u0647 \u062a\u0648\u0636\u06cc\u062d \u062f\u0627\u062f\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a\u060c \u067e\u06cc\u0634 \u0631\u0648\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">2.2. \u067e\u0631\u0648\u062a\u06a9\u0644 \u0627\u0635\u0644\u0627\u062d \u0634\u062f\u0647 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u0628\u0627 FastDNA SPIN<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<h4>1.2.2. \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627 \u0628\u0631\u0627\u06cc \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc\u06cc \u06a9\u0647 \u0628\u0627\u06cc\u062f \u0627\u0633\u062a\u062e\u0631\u0627\u062c \u0634\u0648\u0646\u062f \u0631\u0627 \u0627\u0632 \u0645\u0631\u062d\u0644\u0647 1-4-11 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u06a9\u0646\u06cc\u062f \u0648 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062e\u0627\u0644\u06cc FastPrep \u0631\u0627 \u0628\u0647\u200c\u0639\u0646\u0648\u0627\u0646 \u06a9\u0646\u062a\u0631\u0644 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u0627\u0636\u0627\u0641\u0647 \u0646\u0645\u0627\u06cc\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>2-2-2\u00a0\u00a0 \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0644\u0648\u0644\u0647\u200c\u0647\u0627 \u0628\u0627 \u0627\u0641\u0632\u0648\u062f\u0646 beads<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0647 \u062f\u0642\u062a \u00a0beads \u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0647\u0631 \u0644\u0648\u0644\u0647 Lysing Matrix E\u060c \u062a\u0623\u0645\u06cc\u0646\u200c\u0634\u062f\u0647 \u062f\u0631 \u06a9\u06cc\u062a (FastDNA Spin) \u0631\u0627 \u0628\u0647 \u0647\u0631 \u0644\u0648\u0644\u0647 \u0622\u0645\u0627\u062f\u0647\u200c\u0634\u062f\u0647 \u062f\u0631 \u0645\u0631\u062d\u0644\u0647 2-1 \u0645\u0646\u062a\u0642\u0644 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>3.2.2. \u0627\u0641\u0632\u0648\u062f\u0646 \u0628\u0627\u0641\u0631<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]<span style=\"font-size: 14pt;\">\u0645\u0642\u062f\u0627\u0631 222 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0628\u0627\u0641\u0631 MT \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f. <\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>4.2.2. \u062a\u0646\u0638\u06cc\u0645 \u062d\u062c\u0645 \u0646\u0647\u0627\u06cc\u06cc \u0628\u0627 \u0628\u0627\u0641\u0631 \u0633\u062f\u06cc\u0645 \u0641\u0633\u0641\u0627\u062a \u0648 \u0628\u0633\u062a\u0646 \u0644\u0648\u0644\u0647<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u0642\u062f\u0627\u0631 \u06f9\u06f7\u06f8 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 (\u06cc\u0627 \u06a9\u0645\u062a\u0631) \u0628\u0627\u0641\u0631 \u0633\u062f\u06cc\u0645 \u0641\u0633\u0641\u0627\u062a \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0628\u0647 \u062d\u062f\u0627\u06a9\u062b\u0631 \u0627\u0631\u062a\u0641\u0627\u0639 \u067e\u0631 \u0634\u062f\u0646 \u0644\u0648\u0644\u0647 \u0628\u0631\u0633\u06cc\u062f\u061b \u0628\u0647\u200c\u0637\u0648\u0631\u06cc\u200c\u06a9\u0647 \u062d\u062f\u0627\u0642\u0644 \u06f1 \u0633\u0627\u0646\u062a\u06cc\u200c\u0645\u062a\u0631 \u0641\u0636\u0627\u06cc \u062e\u0627\u0644\u06cc \u0627\u0632 \u0647\u0648\u0627 \u062f\u0631 \u0628\u0627\u0644\u0627\u06cc \u0644\u0648\u0644\u0647 \u0628\u0627\u0642\u06cc \u0628\u0645\u0627\u0646\u062f \u062a\u0627 \u0641\u0631\u0622\u06cc\u0646\u062f \u0636\u0631\u0628\u0647\u200c\u0632\u062f\u0646 \u0628\u0647 \u062f\u0627\u0646\u0647\u200c\u0647\u0627 (bead-beating) \u0628\u0647\u200c\u0637\u0648\u0631 \u0645\u0624\u062b\u0631 \u0627\u0646\u062c\u0627\u0645 \u0634\u0648\u062f (\u062a\u0635\u0648\u06cc\u0631 5). \u0633\u067e\u0633 \u062f\u0631\u067e\u0648\u0634 \u0644\u0648\u0644\u0647 \u0631\u0627 \u0645\u062d\u06a9\u0645 \u0628\u0628\u0646\u062f\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][vc_single_image image=&#8221;30076&#8243; img_size=&#8221;full&#8221; add_caption=&#8221;yes&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>5.2.2. \u0627\u0633\u062a\u062e\u0631\u0627\u062c \u0645\u06a9\u0627\u0646\u06cc\u06a9\u06cc DNA \u0628\u0627 bead-beating<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u00a0\u00a0\u0631\u0627 \u0628\u0647 \u062f\u0633\u062a\u06af\u0627\u0647 \u00a0FastPrep-24 \u0645\u0646\u062a\u0642\u0644 \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0627 \u0631\u0648\u0634 bead-beating\u060c \u0628\u0627 \u062a\u0646\u0638\u06cc\u0645 \u06f6.\u06f5 \u0645\u062a\u0631 \u0628\u0631 \u062b\u0627\u0646\u06cc\u0647 (\u062d\u062f\u0648\u062f \u06f4\u06f0\u06f0\u06f0 \u062f\u0648\u0631 \u062f\u0631 \u062f\u0642\u06cc\u0642\u0647) \u0628\u0647 \u0645\u062f\u062a \u06f6\u06f0 \u062b\u0627\u0646\u06cc\u0647 \u0647\u0645\u0648\u0698\u0646\u06cc\u0632\u0647 \u06a9\u0646\u06cc\u062f. \u0628\u0644\u0627\u0641\u0627\u0635\u0644\u0647 \u067e\u0633 \u0627\u0632 \u0627\u062a\u0645\u0627\u0645 \u06a9\u0627\u0631\u060c \u0646\u06af\u0647\u062f\u0627\u0631\u0646\u062f\u0647 \u0644\u0648\u0644\u0647\u200c\u0647\u0627 \u0631\u0627 \u0627\u0632 \u062f\u0633\u062a\u06af\u0627\u0647 \u062e\u0627\u0631\u062c \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0647 \u0645\u062f\u062a \u06f3 \u062f\u0642\u06cc\u0642\u0647 \u0631\u0648\u06cc \u06cc\u062e \u0642\u0631\u0627\u0631 \u062f\u0647\u06cc\u062f. \u0633\u067e\u0633 \u0646\u06af\u0647\u062f\u0627\u0631\u0646\u062f\u0647 \u0631\u0627 \u062f\u0648\u0628\u0627\u0631\u0647 \u0628\u0647 \u062f\u0633\u062a\u06af\u0627\u0647 \u0628\u0627\u0632\u06af\u0631\u062f\u0627\u0646\u062f\u0647 \u0648 \u0645\u0631\u0627\u062d\u0644 \u00a0bead-beating \u0648 \u0642\u0631\u0627\u0631 \u062f\u0627\u062f\u0646 \u0631\u0648\u06cc \u06cc\u062e \u0631\u0627 \u062a\u06a9\u0631\u0627\u0631 \u06a9\u0646\u06cc\u062f .\u0644\u0648\u0644\u0647\u200c\u0647\u0627 \u0631\u0627 \u0627\u0632 \u0646\u06af\u0647\u062f\u0627\u0631\u0646\u062f\u0647 \u062e\u0627\u0631\u062c \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0647 \u0645\u062f\u062a \u06f1\u06f5 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06f1\u06f4,\u06f0\u06f0\u06f0 g \u00d7 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>6.2.2. \u0627\u0641\u0632\u0648\u062f\u0646 PPS<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a \u0631\u0627 \u0628\u0647 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u06f2 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u062a\u0645\u06cc\u0632 \u0645\u0646\u062a\u0642\u0644 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u06f2\u06f5\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u00a0<span style=\"color: #0000ff;\"><sup>[2]<\/sup><\/span> PPS\u00a0(\u0645\u062d\u0644\u0648\u0644 \u0631\u0633\u0648\u0628 \u062f\u0647\u0646\u062f\u0647 \u067e\u0631\u0648\u062a\u0626\u06cc\u0646) \u00a0\u0627\u0636\u0627\u0641\u0647 \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0627 \u062f\u0633\u062a\u060c \u0644\u0648\u0644\u0647 \u0631\u0627 \u06f1\u06f0 \u0628\u0627\u0631 \u0648\u0627\u0631\u0648\u0646\u0647 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u062e\u0644\u0648\u0637 \u0634\u0648\u062f .<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>7.2.2. \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0648 \u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0647 \u0645\u062f\u062a \u06f5 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06f1\u06f4,\u06f0\u06f0\u06f0 g \u00d7 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f . \u0633\u067e\u0633 \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a \u0631\u0627 \u0628\u0647 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u0641\u0627\u0644\u06a9\u0648\u0646 \u06f1\u06f5 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u062a\u0645\u06cc\u0632 \u062d\u0627\u0648\u06cc \u06f1 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u00a0Binding Matrix \u0645\u0639\u0644\u0642 \u0645\u0646\u062a\u0642\u0644 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>8.2.2.\u00a0 \u0622\u0645\u06cc\u062e\u062a\u0646 \u0633\u0648\u0633\u067e\u0627\u0646\u0633\u06cc\u0648\u0646 \u0648 \u0631\u0633\u0648\u0628\u200c\u062f\u0627\u062f\u0646 \u0645\u0627\u062a\u0631\u06cc\u0633 \u0633\u06cc\u0644\u06cc\u06a9\u0627<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627 \u0631\u0627 \u0631\u0648\u06cc \u062f\u0633\u062a\u06af\u0627\u0647 \u0631\u0648\u062a\u0627\u062a\u0648\u0631 \u0642\u0631\u0627\u0631 \u062f\u0627\u062f\u0647 \u06cc\u0627 \u0628\u0627 \u062f\u0633\u062a \u0628\u0647 \u0645\u062f\u062a \u06f2 \u062f\u0642\u06cc\u0642\u0647 \u0648\u0627\u0631\u0648\u0646\u0647 \u06a9\u0646\u06cc\u062f\u060c \u0633\u067e\u0633 \u0627\u062c\u0627\u0632\u0647 \u062f\u0647\u06cc\u062f \u0645\u0627\u062a\u0631\u06cc\u0633 \u0633\u06cc\u0644\u06cc\u06a9\u0627 \u0628\u0647 \u0645\u062f\u062a \u06f3 \u062f\u0642\u06cc\u0642\u0647 \u062a\u0647\u200c\u0646\u0634\u06cc\u0646 \u0634\u0648\u062f . \u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u06f1\u06f5 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0631\u0627 \u0628\u0647 \u0637\u0648\u0631 \u06a9\u0648\u062a\u0627\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06f1,\u06f0\u06f0\u06f0 g \u00d7 \u0628\u0647 \u0645\u062f\u062a \u06f1 \u062f\u0642\u06cc\u0642\u0647 \u062f\u0631 \u0631\u0648\u062a\u0648\u0631\u0647\u0627\u06cc \u0632\u0627\u0648\u06cc\u0647 \u0645\u062a\u063a\u06cc\u0631 \u06cc\u0627 \u0633\u0648\u06cc\u06cc\u0646\u06af \u00a0\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>9.2.2. \u062c\u062f\u0627\u06a9\u0631\u062f\u0646 \u0645\u0642\u062f\u0627\u0631\u06cc \u0627\u0632 \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a \u067e\u0633 \u0627\u0632 \u0631\u0633\u0648\u0628<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u062c\u0645\u0648\u0639\u0627\u064b \u06f1.\u06f4 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a \u0627\u0632 \u0647\u0631 \u0644\u0648\u0644\u0647 \u0628\u0631\u062f\u0627\u0634\u062a\u0647 \u0648 \u062f\u0648\u0631 \u0628\u0631\u06cc\u0632\u06cc\u062f (\u062f\u0631 \u062f\u0648 \u0628\u062e\u0634 \u06f7\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631).<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>10.2.2. \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0648 \u0639\u0628\u0648\u0631 \u0646\u0645\u0648\u0646\u0647 \u0627\u0632 SPIN Filter<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u0627\u062a\u0631\u06cc\u0633 \u0631\u0627 \u062f\u0631 \u0633\u0648\u067e\u0631\u0646\u0627\u062a\u0627\u0646\u062a \u0628\u0627\u0642\u06cc\u200c\u0645\u0627\u0646\u062f\u0647 \u062f\u0648\u0628\u0627\u0631\u0647 \u0645\u0639\u0644\u0642 \u06a9\u0631\u062f\u0647 \u0648 \u062d\u062f\u0648\u062f \u06f7\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0627\u0632 \u0622\u0646 \u0631\u0627 \u0628\u0647 SPIN Filter \u00a0\u062f\u0631 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc (catch tube) \u0645\u0646\u062a\u0642\u0644 \u06a9\u0646\u06cc\u062f. \u0628\u0647 \u0645\u062f\u062a \u06f1 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06f1\u06f4,\u06f0\u06f0\u06f0 g \u00d7 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f. \u0644\u0648\u0644\u0647 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u0631\u0627 \u062e\u0627\u0644\u06cc \u06a9\u0631\u062f\u0647 \u0648 \u0647\u0631 \u0645\u062e\u0644\u0648\u0637 \u0628\u0627\u0642\u06cc\u200c\u0645\u0627\u0646\u062f\u0647 \u0631\u0627 \u062f\u0648\u0628\u0627\u0631\u0647 \u0628\u0647 SPIN Filter \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0647\u0645\u0627\u0646\u0646\u062f \u0642\u0628\u0644 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0631\u062f\u0647 \u0648 \u062f\u0648\u0628\u0627\u0631\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u0631\u0627 \u062e\u0627\u0644\u06cc \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>11.2.2. \u0627\u0641\u0632\u0648\u062f\u0646 \u0628\u0627\u0641\u0631 \u0634\u0633\u062a\u0634\u0648 \u0648 \u0645\u062e\u0644\u0648\u0637 \u06a9\u0631\u062f\u0646<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0647 \u0647\u0631 \u0641\u06cc\u0644\u062a\u0631\u060c \u06f5\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 SWES-M \u0622\u0645\u0627\u062f\u0647 \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f. \u0628\u0627 \u062f\u0642\u062a\u060c \u0628\u0627 \u067e\u06cc\u067e\u062a \u0628\u0647 \u0622\u0631\u0627\u0645\u06cc \u0645\u062e\u0644\u0648\u0637 \u0631\u0627 \u0645\u0639\u0644\u0642 \u06a9\u0646\u06cc\u062f .<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>12.2.2. \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0648 \u062a\u0639\u0648\u06cc\u0636 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]<span style=\"font-size: 14pt;\">\u0628\u0647 \u0645\u062f\u062a \u06f1 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06f1\u06f4,\u06f0\u06f0\u06f0 g \u00d7 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f\u060c \u0644\u0648\u0644\u0647 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u0631\u0627 \u062e\u0627\u0644\u06cc \u06a9\u0631\u062f\u0647 \u0648 \u062c\u0627\u06cc\u06af\u0632\u06cc\u0646 \u06a9\u0646\u06cc\u062f.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>13.2.2. \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0646\u0647\u0627\u06cc\u06cc<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0647 \u0645\u062f\u062a \u06f2 \u062f\u0642\u06cc\u0642\u0647 \u0628\u0627 \u0633\u0631\u0639\u062a \u06f1\u06f4,\u06f0\u06f0\u06f0 g \u00d7 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u0627\u062a\u0631\u06cc\u0633 \u062e\u0634\u06a9 \u0634\u0648\u062f. \u0644\u0648\u0644\u0647 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u0631\u0627 \u062f\u0648\u0631 \u0627\u0646\u062f\u0627\u062e\u062a\u0647 \u0648 \u0628\u0627 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u062c\u062f\u06cc\u062f \u062c\u0627\u06cc\u06af\u0632\u06cc\u0646 \u06a9\u0646\u06cc\u062f .<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>14.2.2. \u062e\u0634\u06a9 \u06a9\u0631\u062f\u0646 \u0641\u06cc\u0644\u062a\u0631 \u062f\u0631 \u062f\u0645\u0627\u06cc \u0627\u062a\u0627\u0642<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]<span style=\"font-size: 14pt;\">\u00a0\u0641\u06cc\u0644\u062a\u0631 \u0631\u0627 \u0628\u0647 \u0645\u062f\u062a \u06f5 \u062f\u0642\u06cc\u0642\u0647 \u062f\u0631 \u062f\u0645\u0627\u06cc \u0627\u062a\u0627\u0642 \u0648 \u0641\u0636\u0627\u06cc \u0628\u0627\u0632 \u062e\u0634\u06a9 \u06a9\u0646\u06cc\u062f.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>15.2.2. \u0634\u0633\u062a\u0634\u0648\u06cc DNA \u0628\u0627 \u0645\u062d\u0644\u0648\u0644 DES \u0648 \u062d\u0631\u0627\u0631\u062a\u200c\u062f\u0647\u06cc<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u00a0751 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 <span style=\"color: #0000ff;\"><sup>[3]<\/sup><\/span> DES (\u0645\u062d\u0644\u0648\u0644 \u0634\u0633\u062a \u0648 \u0634\u0648 DNA) \u0628\u0647 \u0645\u0627\u062a\u0631\u06cc\u0633 \u062f\u0627\u062e\u0644 SPIN Filter \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f. \u0645\u0627\u062a\u0631\u06cc\u0633 Binding \u00a0\u00a0\u0631\u0627 \u0628\u0627 \u0646\u0648\u06a9 \u067e\u06cc\u067e\u062a \u0628\u0647 \u0622\u0631\u0627\u0645\u06cc \u0647\u0645 \u0628\u0632\u0646\u06cc\u062f \u062a\u0627 \u062f\u0648\u0628\u0627\u0631\u0647 \u0645\u0639\u0644\u0642 \u0634\u0648\u062f. \u0633\u067e\u0633 \u0628\u0647 \u0645\u062f\u062a \u06f5 \u062f\u0642\u06cc\u0642\u0647 \u062f\u0631 \u0628\u0644\u0648\u06a9 \u062d\u0631\u0627\u0631\u062a\u06cc \u0628\u0627 \u062f\u0645\u0627\u06cc \u06f5\u06f5 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u0642\u0631\u0627\u0631 \u062f\u0647\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>16.2.2. \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u0648 \u062e\u0627\u0631\u062c \u06a9\u0631\u062f\u0646 \u0641\u06cc\u0644\u062a\u0631<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0627 \u0633\u0631\u0639\u062a \u06f1\u06f4,\u06f0\u06f0\u06f0 g \u00d7 \u0628\u0647 \u0645\u062f\u062a \u06f1 \u062f\u0642\u06cc\u0642\u0647 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 DNA \u0627\u0633\u062a\u062e\u0631\u0627\u062c\u200c\u0634\u062f\u0647 \u062c\u0645\u0639\u200c\u0622\u0648\u0631\u06cc \u0634\u0648\u062f\u060c \u0633\u067e\u0633 SPIN Filter \u00a0\u0631\u0627 \u062f\u0648\u0631 \u0628\u06cc\u0646\u062f\u0627\u0632\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>17.2.2. \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc DNA<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u00a0\u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc DNA \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0631\u0627 \u0645\u06cc\u200c\u062a\u0648\u0627\u0646 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f4 \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u062a\u0627 \u06f2 \u0631\u0648\u0632 \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u06a9\u0631\u062f \u06cc\u0627 \u0628\u0631\u0627\u06cc \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u0637\u0648\u0644\u0627\u0646\u06cc\u200c\u062a\u0631 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f2\u06f0 -\u06cc\u0627 \u06f8\u06f0- \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u0642\u0628\u0644 \u0627\u0632 \u0627\u0646\u062c\u0627\u0645 \u0645\u0631\u062d\u0644\u0647 \u062a\u0634\u062e\u06cc\u0635 Real Time PCR \u00a0\u0630\u062e\u06cc\u0631\u0647 \u06a9\u0631\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h2 style=\"text-align: justify;\"><a name=\"_Toc208648549\"><\/a><span style=\"font-size: 24pt;\">3.\u00a0\u00a0 \u0631\u0648\u0634 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u00a0\u0628\u0647 \u06a9\u0645\u06a9 Real Time PCR<\/span><\/h2>\n[\/vc_column_text][vc_single_image image=&#8221;30077&#8243; img_size=&#8221;full&#8221; add_caption=&#8221;yes&#8221; alignment=&#8221;center&#8221; css=&#8221;&#8221;][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06cc\u0646 \u0631\u0648\u0634 \u062a\u062d\u0644\u06cc\u0644\u06cc\u060c \u06cc\u06a9 \u0631\u0648\u0634 \u00a0Real Time PCR \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u00a0C. cayetanensis \u0627\u0631\u0627\u0626\u0647 \u0645\u06cc\u200c\u062f\u0647\u062f. \u0627\u06cc\u0646 \u0631\u0648\u0634 \u062c\u0627\u06cc\u06af\u0632\u06cc\u0646 \u0631\u0648\u0634\u200c\u0647\u0627\u06cc \u00a0Real Time PCR \u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0641\u0635\u0644\u200c\u0647\u0627\u06cc A19 \u0648 B19 \u06a9\u062a\u0627\u0628 FDA BAM \u0645\u06cc\u200c\u0634\u0648\u062f. \u067e\u0631\u0648\u062a\u06a9\u0644 \u00a0Real Time PCR \u06a9\u0647 \u062f\u0631 \u0627\u062f\u0627\u0645\u0647 \u062a\u0648\u0636\u06cc\u062d \u062f\u0627\u062f\u0647 \u0634\u062f\u0647\u060c \u0645\u0632\u0627\u06cc\u0627\u06cc \u0645\u062a\u0639\u062f\u062f\u06cc \u062f\u0627\u0631\u062f\u060c \u0627\u0632 \u062c\u0645\u0644\u0647 \u0627\u0641\u0632\u0627\u06cc\u0634 \u0648\u06cc\u0698\u06af\u06cc \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0639\u0645\u0644 \u06a9\u0631\u062f\u0646 . (specificity) \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0645\u062b\u0627\u0644 \u0627\u06cc\u0646 \u0631\u0648\u0634 \u0645\u0648\u062c\u0628 \u06a9\u0627\u0647\u0634 \u0627\u062d\u062a\u0645\u0627\u0644 \u0622\u0644\u0648\u062f\u06af\u06cc \u0645\u062d\u06cc\u0637 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 \u062a\u0648\u0633\u0637 \u0622\u0645\u067e\u0644\u06cc\u06a9\u0648\u0646\u200c\u0647\u0627\u060c \u06a9\u0647 \u0645\u0639\u0645\u0648\u0644\u0627\u064b \u0628\u0627 PCR \u062a\u0648 \u062f\u0631 \u062a\u0648 (nested PCR) \u0627\u06cc\u062c\u0627\u062f \u0645\u06cc\u200c\u0634\u0648\u0646\u062f\u060c \u062e\u0648\u0627\u0647\u062f \u0634\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06cc\u0646 \u0631\u0648\u0634 \u062a\u062d\u0644\u06cc\u0644\u06cc\u060c \u06cc\u06a9 \u0631\u0648\u0634 \u00a0Real Time PCR \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u00a0C. cayetanensis \u0627\u0631\u0627\u0626\u0647 \u0645\u06cc\u200c\u062f\u0647\u062f. \u0627\u06cc\u0646 \u0631\u0648\u0634 \u062c\u0627\u06cc\u06af\u0632\u06cc\u0646 \u0631\u0648\u0634\u200c\u0647\u0627\u06cc \u00a0Real Time PCR \u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0641\u0635\u0644\u200c\u0647\u0627\u06cc A19 \u0648 B19 \u06a9\u062a\u0627\u0628 FDA BAM \u0645\u06cc\u200c\u0634\u0648\u062f. \u067e\u0631\u0648\u062a\u06a9\u0644 \u00a0Real Time PCR \u06a9\u0647 \u062f\u0631 \u0627\u062f\u0627\u0645\u0647 \u062a\u0648\u0636\u06cc\u062d \u062f\u0627\u062f\u0647 \u0634\u062f\u0647\u060c \u0645\u0632\u0627\u06cc\u0627\u06cc \u0645\u062a\u0639\u062f\u062f\u06cc \u062f\u0627\u0631\u062f\u060c \u0627\u0632 \u062c\u0645\u0644\u0647 \u0627\u0641\u0632\u0627\u06cc\u0634 \u0648\u06cc\u0698\u06af\u06cc \u0627\u062e\u062a\u0635\u0627\u0635\u06cc \u0639\u0645\u0644 \u06a9\u0631\u062f\u0646. (specificity) \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u0645\u062b\u0627\u0644 \u0627\u06cc\u0646 \u0631\u0648\u0634 \u0645\u0648\u062c\u0628 \u06a9\u0627\u0647\u0634 \u0627\u062d\u062a\u0645\u0627\u0644 \u0622\u0644\u0648\u062f\u06af\u06cc \u0645\u062d\u06cc\u0637 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647 \u062a\u0648\u0633\u0637 \u0622\u0645\u067e\u0644\u06cc\u06a9\u0648\u0646\u200c\u0647\u0627\u060c \u06a9\u0647 \u0645\u0639\u0645\u0648\u0644\u0627\u064b \u0628\u0627 PCR \u062a\u0648 \u062f\u0631 \u062a\u0648 (nested PCR) \u0627\u06cc\u062c\u0627\u062f \u0645\u06cc\u200c\u0634\u0648\u0646\u062f\u060c \u062e\u0648\u0627\u0647\u062f \u0634\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0622\u0632\u0645\u0627\u06cc\u0634 Real-Time PCR \u06cc\u06a9 \u0648\u0627\u06a9\u0646\u0634 \u062f\u0648\u06af\u0627\u0646\u0647 (Duplex) \u0627\u0633\u062a \u06a9\u0647 \u062f\u0631 \u0622\u0646 \u0698\u0646 \u0645\u06cc\u062a\u0648\u06a9\u0646\u062f\u0631\u06cc \u0686\u0646\u062f\u0646\u0633\u062e\u0647\u200c\u0627\u06cc Cyclospora cayetanensis \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0647\u062f\u0641 \u0642\u0631\u0627\u0631 \u0645\u06cc\u200c\u06af\u06cc\u0631\u062f. \u0647\u0645\u0686\u0646\u06cc\u0646 \u06cc\u06a9 \u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc \u062a\u0642\u0648\u06cc\u062a (<span style=\"color: #0000ff;\"><sup>[4]<\/sup><\/span>IAC) \u0647\u0645 \u0628\u0647 \u0648\u0627\u06a9\u0646\u0634 \u0627\u0636\u0627\u0641\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f \u062a\u0627 \u0627\u06af\u0631 \u062a\u0631\u06a9\u06cc\u0628\u0627\u062a \u0645\u0648\u062c\u0648\u062f \u062f\u0631 \u0646\u0645\u0648\u0646\u0647 \u063a\u0630\u0627\u06cc\u06cc \u0628\u0627\u0639\u062b \u0645\u0647\u0627\u0631 \u0648\u0627\u06a9\u0646\u0634 \u0634\u062f\u0646\u062f\u060c \u0642\u0627\u0628\u0644 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0628\u0627\u0634\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">\u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc \u062a\u0642\u0648\u06cc\u062a<\/span><\/h3>\n[\/vc_column_text][vc_message message_box_color=&#8221;pink&#8221; icon_fontawesome=&#8221;fa fa-solid fa-hand-point-right&#8221; css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 18pt;\"><strong>\u0628\u06cc\u0634\u062a\u0631 \u0628\u062f\u0627\u0646\u06cc\u062f<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc \u062a\u0642\u0648\u06cc\u062a \u00a0(Internal Amplification Control \u2013 IAC)<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06cc\u0646 \u06a9\u0646\u062a\u0631\u0644 \u06cc\u06a9 \u0642\u0637\u0639\u0647 DNA \u06cc\u0627 RNA \u0645\u0635\u0646\u0648\u0639\u06cc \u06cc\u0627 \u0637\u0628\u06cc\u0639\u06cc \u0627\u0633\u062a \u06a9\u0647 \u0647\u0645\u0631\u0627\u0647 \u0646\u0645\u0648\u0646\u0647 \u0627\u0635\u0644\u06cc \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634 PCR \u0627\u0636\u0627\u0641\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f \u062a\u0627 \u0627\u0637\u0645\u06cc\u0646\u0627\u0646 \u062d\u0627\u0635\u0644 \u0634\u0648\u062f \u06a9\u0647 \u0641\u0631\u0622\u06cc\u0646\u062f \u062a\u06a9\u062b\u06cc\u0631 (Amplification) \u0628\u0647 \u062f\u0631\u0633\u062a\u06cc \u0627\u0646\u062c\u0627\u0645 \u0634\u062f\u0647 \u0627\u0633\u062a.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0648\u06cc\u0698\u06af\u06cc\u200c\u0647\u0627 \u0648 \u06a9\u0627\u0631\u0628\u0631\u062f\u0647\u0627:<\/span><\/p>\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0634\u062e\u06cc\u0635 \u0627\u062e\u062a\u0644\u0627\u0644\u0627\u062a \u0648\u0627\u06a9\u0646\u0634: \u0627\u06af\u0631 PCR \u0628\u0647 \u0647\u0631 \u062f\u0644\u06cc\u0644\u06cc (\u0645\u062b\u0644\u0627\u064b \u0648\u062c\u0648\u062f \u0645\u0647\u0627\u0631\u06a9\u0646\u0646\u062f\u0647\u200c\u0647\u0627 \u062f\u0631 \u0646\u0645\u0648\u0646\u0647) \u0645\u062a\u0648\u0642\u0641 \u0634\u0648\u062f \u06cc\u0627 \u0646\u0627\u06a9\u0627\u0631\u0622\u0645\u062f \u0628\u0627\u0634\u062f\u060c \u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc \u0646\u0634\u0627\u0646 \u0645\u06cc\u200c\u062f\u0647\u062f \u06a9\u0647 \u0646\u062a\u06cc\u062c\u0647 \u0645\u0646\u0641\u06cc \u0648\u0627\u0642\u0639\u06cc \u0627\u0633\u062a \u06cc\u0627 \u0628\u0647 \u062f\u0644\u06cc\u0644 \u0645\u0634\u06a9\u0644 \u062a\u06a9\u0646\u06cc\u06a9\u06cc \u0645\u0646\u0641\u06cc \u0634\u062f\u0647 \u0627\u0633\u062a.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u0641\u0632\u0627\u06cc\u0634 \u0627\u0637\u0645\u06cc\u0646\u0627\u0646: \u00a0\u0648\u062c\u0648\u062f IAC \u0628\u0627\u0639\u062b \u0645\u06cc\u200c\u0634\u0648\u062f \u06a9\u0647 \u0646\u062a\u0627\u06cc\u062c \u0622\u0632\u0645\u0627\u06cc\u0634 \u0642\u0627\u0628\u0644 \u0627\u0639\u062a\u0645\u0627\u062f\u062a\u0631 \u0628\u0627\u0634\u0646\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u0639\u0645\u0648\u0644\u0627\u064b \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634\u200c\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635\u06cc \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0645\u0627\u0646\u0646\u062f \u062a\u0634\u062e\u06cc\u0635 \u0648\u06cc\u0631\u0648\u0633\u200c\u0647\u0627\u060c \u0628\u0627\u06a9\u062a\u0631\u06cc\u200c\u0647\u0627 \u06cc\u0627 \u0627\u0646\u06af\u0644\u200c\u0647\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_message][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><span style=\"font-size: 18pt;\">1.3. \u062a\u062c\u0647\u06cc\u0632\u0627\u062a \u0648 \u0645\u0648\u0627\u062f \u0645\u0635\u0631\u0641\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u06cc\u0633\u062a\u0645 Applied Biosystems 7500 Fast Real-Time PCR \u0628\u0627 \u0646\u0633\u062e\u0647\u200c\u0647\u0627\u06cc \u0646\u0631\u0645\u200c\u0627\u0641\u0632\u0627\u0631\u06cc \u06f1.\u06f4\u060c \u06f2.\u06f0 \u06cc\u0627 \u06f2.\u06f3 \u0648 \u0646\u0633\u062e\u0647\u200c\u0647\u0627\u06cc \u062c\u062f\u06cc\u062f\u062a\u0631<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627 \u0648 \u067e\u0644\u06cc\u062a \u0648\u0627\u06a9\u0646\u0634 \u0642\u0627\u0628\u0644 \u0627\u0633\u062a\u0641\u0627\u062f\u0647:<\/span><\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647 \u0627\u0633\u062a\u0631\u06cc\u067e \u0628\u0627 \u062d\u062c\u0645 \u06f0.\u06f1 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0648 .MicroAmp\u00ae Optical 8-Cap Strips \u0634\u0645\u0627\u0631\u0647\u200c\u0647\u0627\u06cc \u06a9\u0627\u062a\u0627\u0644\u0648\u06af: 4358293 \u0648 \u00a04323032 (ThermoFisher Scientific) \u06cc\u0627 \u0645\u0639\u0627\u062f\u0644 \u0622\u0646.<\/span><\/li>\n<li><span style=\"font-size: 14pt;\">\u067e\u0644\u06cc\u062a 96 \u062e\u0627\u0646\u0647 \u00a0\u00a0MicroAmp\u00ae Fast Optical\u060c \u062d\u062c\u0645 \u06f0.\u06f1 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631 \u0648 \u0641\u06cc\u0644\u0645 \u0686\u0633\u0628\u0646\u062f\u0647 Optical .MicroAmp\u00ae \u0634\u0645\u0627\u0631\u0647\u200c\u0647\u0627\u06cc \u06a9\u0627\u062a\u0627\u0644\u0648\u06af \u00a04346907: \u0648 4311971 (ThermoFisher Scientific) \u00a0\u06cc\u0627 \u0645\u0639\u0627\u062f\u0644 \u0622\u0646.<\/span><\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0631\u0648\u0645\u06cc\u0632\u06cc \u06a9\u0647 \u0642\u0627\u062f\u0631 \u0628\u0647 \u0686\u0631\u062e\u0634 \u067e\u0644\u06cc\u062a 96 \u062e\u0627\u0646\u0647 \u0628\u0627\u0634\u062f \u06cc\u0627 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0645\u0646\u0627\u0633\u0628 \u0628\u0631\u0627\u06cc \u0644\u0648\u0644\u0647 \u0647\u0627\u06cc \u0627\u0633\u062a\u0631\u06cc\u067e<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u0631\u0648\u0645\u06cc\u0632\u06cc \u06a9\u0647 \u0645\u0646\u0627\u0633\u0628 \u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0645\u06cc\u06a9\u0631\u0648\u062a\u06cc\u0648\u0628 \u06f1.\u06f5 \u062a\u0627 \u06f2.\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0628\u0627\u0634\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u06cc\u06a9\u0631\u0648\u067e\u06cc\u067e\u062a\u200c\u0647\u0627.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u0631\u067e\u06cc\u067e\u062a\u200c\u0647\u0627\u06cc \u0645\u0642\u0627\u0648\u0645 \u062f\u0631 \u0628\u0631\u0627\u0628\u0631 \u0622\u0626\u0631\u0648\u0633\u0644.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0633\u062a\u06a9\u0634 \u0644\u0627\u062a\u06a9\u0633 \u06cc\u0627 \u0646\u06cc\u062a\u0631\u06cc\u0644.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0648\u0631\u062a\u06a9\u0633 \u0645\u06cc\u06a9\u0633\u0631.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0645\u06cc\u06a9\u0631\u0648\u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06f1.\u06f5 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631\u06cc \u0641\u0627\u0642\u062f DNase \u0648 \u0628\u0627 \u0648\u06cc\u0698\u06af\u06cc \u0686\u0633\u0628\u0646\u062f\u06af\u06cc \u06a9\u0645. (low retention)<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><span style=\"font-size: 18pt;\">2.3. \u0648\u0627\u06a9\u0646\u0634\u200c\u06af\u0631\u0647\u0627 (Reagents) \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">PrimeTime\u2122 Gene Expression (IDT) \u00a0\u0645\u0633\u062a\u0631 \u0645\u06cc\u06a9\u0633 \u0634\u0645\u0627\u0631\u0647 \u06a9\u0627\u062a\u0627\u0644\u0648\u06af 1055770 (\u06f1 \u00d7 \u06f1 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631) \u06cc\u0627 1055772 (\u06f1 \u00d7 \u06f5 \u0645\u06cc\u0644\u06cc\u200c\u0644\u06cc\u062a\u0631).<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0648\u0627\u06a9\u0646\u0634\u06af\u0631 \u0634\u0627\u0645\u0644 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u062f\u0627\u06af\u0627\u0646\u0647 \u062d\u0627\u0648\u06cc \u0631\u0646\u06af \u0645\u0631\u062c\u0639 (reference dye \u2013 ROX) \u0627\u0633\u062a \u06a9\u0647 \u0642\u0628\u0644 \u0627\u0632 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0628\u0627\u06cc\u062f \u0637\u0628\u0642 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0633\u0627\u0632\u0646\u062f\u0647 \u0628\u0647 Master Mix \u0627\u0636\u0627\u0641\u0647 \u0634\u0648\u062f. \u0627\u06cc\u0646 \u06a9\u0627\u0631 \u0628\u0631\u0627\u06cc \u062a\u0646\u0638\u06cc\u0645 \u0633\u0637\u062d low reference dye \u062f\u0631 \u062f\u0633\u062a\u06af\u0627\u0647 Applied Biosystems 7500 Fast Real-Time PCR \u00a0\u0644\u0627\u0632\u0645 \u0627\u0633\u062a.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0627\u0641\u0631 TE \u0627\u0633\u062a\u0631\u06cc\u0644 \u0648 \u0641\u0627\u0642\u062f DNase \u0628\u0627 \u06f7.\u06f5 pH ( \u06f1\u06f0 \u0645\u06cc\u0644\u06cc\u200c\u0645\u0648\u0644\u0627\u0631 Tris\u060c \u06f0.\u06f1 \u0645\u06cc\u0644\u06cc\u200c\u0645\u0648\u0644\u0627\u0631 EDTA\u060c 8 pH)<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 1. \u062a\u0631\u06a9\u06cc\u0628\u0627\u062a Tris-EDTA<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%; height: 175px;\">\n<tbody>\n<tr style=\"height: 61px;\">\n<td style=\"width: 50%; height: 61px; text-align: left;\"><span style=\"font-size: 14pt;\"><strong>\u062d\u062c\u0645<\/strong><\/span><\/td>\n<td style=\"width: 49.875%; height: 61px;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u0645\u062d\u0644\u0648\u0644 \u0645\u0635\u0631\u0641\u06cc<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<tr style=\"height: 38px;\">\n<td style=\"width: 50%; height: 38px; text-align: center;\"><span style=\"font-size: 14pt;\">100\u00b5L<\/span><\/td>\n<td style=\"width: 49.875%;\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062a\u0631\u06cc\u0633 \u06f1 \u0645\u0648\u0644\u0627\u0631 \u0628\u0627 pH 7.5<\/span><\/p>\n<\/td>\n<\/tr>\n<tr style=\"height: 38px;\">\n<td style=\"width: 50%; height: 38px; text-align: center;\"><span style=\"font-size: 14pt;\">20\u00b5L<\/span><\/td>\n<td style=\"width: 49.875%;\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u00a0EDTA \u0628\u0627 \u063a\u0644\u0638\u062a \u06f0.\u06f0\u06f5 \u0645\u0648\u0644\u0627\u0631<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 50%;\" width=\"312\">\n<p style=\"text-align: right;\"><span style=\"font-size: 14pt;\">9.88mL<\/span><\/p>\n<\/td>\n<td style=\"width: 49.875%;\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u0622\u0628 \u0645\u062e\u0635\u0648\u0635 PCR (\u0639\u0627\u0631\u06cc \u0627\u0632 RNAs\/DANs)<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u060c \u0645\u062d\u0644\u0648\u0644 \u0627\u0633\u062a\u0648\u06a9 \u06f5\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0645\u0648\u0644\u0627\u0631 (\u062c\u062f\u0648\u0644 2 \u0648 \u062c\u062f\u0648\u0644 3).<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u067e\u0631\u0648\u0628\u200c\u0647\u0627\u060c \u0645\u062d\u0644\u0648\u0644 \u0627\u0633\u062a\u0648\u06a9 \u06f1\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0645\u0648\u0644\u0627\u0631 (\u062c\u062f\u0648\u0644 4).<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0647\u062f\u0641 \u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc \u062a\u0642\u0648\u06cc\u062a (IAC Target: HMultra130-synIAC) \u060c \u0628\u0627 \u063a\u0644\u0638\u062a \u06a9\u0627\u0631\u06cc 1E7 \u0646\u0633\u062e\u0647 \u062f\u0631 \u0647\u0631 \u00a0.\u00b5L \u00a0(\u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0633\u0641\u0627\u0631\u0634 \u0648 \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0631\u0627 \u062f\u0631 \u0628\u062e\u0634 3-6 \u00a0\u0645\u0634\u0627\u0647\u062f\u0647 \u06a9\u0646\u06cc\u062f.)<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a (Mit1AA gblock)\u060c \u0628\u0627 \u063a\u0644\u0638\u062a 5E2 <strong>\u00a0<\/strong>\u0646\u0633\u062e\u0647 \u062f\u0631 \u0647\u0631 . \u00b5L \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0633\u0641\u0627\u0631\u0634 \u0648 \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u062f\u0631 \u0628\u062e\u0634 3-6 \u0645\u0648\u062c\u0648\u062f \u0627\u0633\u062a.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0648\u062c\u0647: \u062f\u0631 \u0627\u06cc\u0646 \u0645\u0642\u0627\u0644\u0647 \u0647\u0631 \u062c\u0627 \u0639\u0628\u0627\u0631\u0627\u062a\u06cc \u0645\u0627\u0646\u0646\u062f 5E2 <strong>\u00a0<\/strong>\u062f\u06cc\u062f\u06cc\u062f\u060c E \u0628\u0647 \u0645\u0639\u0646\u0627\u06cc &#8220;\u0636\u0631\u0628 \u062f\u0631 10 \u0628\u0647 \u062a\u0648\u0627\u0646 &#8230;&#8221; \u0627\u0633\u062a. \u0645\u062b\u0644\u0627 5E2 <strong>\u00a0<\/strong>\u0628\u0647 \u0645\u0639\u0646\u0627\u06cc 5 \u0636\u0631\u0628 \u062f\u0631 \u062f\u0647 \u0628\u0647 \u062a\u0648\u0627\u0646 2 \u06a9\u0647 \u0645\u0639\u0627\u062f\u0644 500 \u0627\u0633\u062a\u060c \u0645\u06cc\u200c\u0628\u0627\u0634\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li><span style=\"font-size: 14pt;\">\u06a9\u0646\u062a\u0631\u0644 \u0645\u0646\u0641\u06cc (\u0622\u0628 \u0641\u0627\u0642\u062fDNase).<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">\u067e\u0631\u0648\u0628\u060c \u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a \u0648 \u06a9\u0646\u062a\u0631\u0644 \u0645\u0646\u0641\u06cc<\/span><\/h3>\n[\/vc_column_text][vc_message message_box_color=&#8221;pink&#8221; icon_fontawesome=&#8221;fa fa-solid fa-hand-point-right&#8221; css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 18pt;\"><strong>\u0628\u06cc\u0634\u062a\u0631 \u0628\u062f\u0627\u0646\u06cc\u062f<\/strong><\/span><\/p>\n<ul style=\"text-align: justify;\">\n<li><span style=\"font-size: 14pt;\"><strong>\u067e\u0631\u0648\u0628<\/strong><strong> (Probe)<\/strong><\/span><\/li>\n<\/ul>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u200c\u0647\u0627\u06cc \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0648 PCR \u0648\u0627\u0642\u0639\u06cc \u00a0(Real-Time PCR)\u060c \u067e\u0631\u0648\u0628 \u06cc\u06a9 \u0642\u0637\u0639\u0647 \u06a9\u0648\u062a\u0627\u0647 DNA \u06cc\u0627 \u00a0RNA \u0628\u0627 \u0628\u0631\u0686\u0633\u0628 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u062a\u0648\u0627\u0644\u06cc \u062e\u0627\u0635 \u0647\u062f\u0641 \u0637\u0631\u0627\u062d\u06cc \u0645\u06cc\u200c\u0634\u0648\u062f.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0648\u06cc\u0698\u06af\u06cc\u200c\u0647\u0627 \u0648 \u06a9\u0627\u0631\u0628\u0631\u062f\u0647\u0627:<\/span><\/p>\n<ul style=\"text-align: justify;\">\n<li><span style=\"font-size: 14pt;\">\u0645\u0639\u0645\u0648\u0644\u0627\u064b \u062f\u0631 \u0648\u0633\u0637 \u062a\u0648\u0627\u0644\u06cc \u0647\u062f\u0641 \u0642\u0631\u0627\u0631 \u0645\u06cc\u200c\u06af\u06cc\u0631\u062f \u0648 \u0641\u0644\u0648\u0631\u0648\u0641\u0648\u0631 (\u0631\u0646\u06af \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633) \u0648 \u06a9\u0648\u0626\u0646\u0686\u0631 (Quencher) \u062f\u0627\u0631\u062f.<\/span><\/li>\n<li><span style=\"font-size: 14pt;\">\u0632\u0645\u0627\u0646\u06cc \u06a9\u0647 \u067e\u0644\u06cc\u0645\u0631\u0627\u0632 \u067e\u0631\u0648\u0628 \u0631\u0627 \u0645\u06cc\u200c\u0634\u06a9\u0646\u062f \u06cc\u0627 \u0628\u0647 \u0622\u0646 \u0645\u062a\u0635\u0644 \u0645\u06cc\u200c\u0634\u0648\u062f\u060c \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0622\u0632\u0627\u062f \u0645\u06cc\u200c\u0634\u0648\u062f \u0648 \u0645\u0642\u062f\u0627\u0631 \u0622\u0646 \u0628\u0627 \u062a\u0639\u062f\u0627\u062f \u0646\u0633\u062e\u0647\u200c\u0647\u0627\u06cc \u0647\u062f\u0641 \u0645\u062a\u0646\u0627\u0633\u0628 \u0627\u0633\u062a.<\/span><\/li>\n<li><span style=\"font-size: 14pt;\">\u0628\u0627\u0639\u062b \u0645\u06cc\u200c\u0634\u0648\u062f \u062a\u0634\u062e\u06cc\u0635 \u062a\u0648\u0627\u0644\u06cc \u062e\u0627\u0635 \u062f\u0642\u06cc\u0642\u200c\u062a\u0631 \u0648 \u062d\u0633\u0627\u0633\u200c\u062a\u0631 \u0627\u0632 \u0631\u0648\u0634\u200c\u0647\u0627\u06cc \u0645\u0639\u0645\u0648\u0644\u06cc PCR \u00a0\u0628\u0627\u0634\u062f.<\/span><\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li><span style=\"font-size: 14pt;\"><strong>\u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a<\/strong><strong> (Positive Control):<\/strong><\/span><\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li><span style=\"font-size: 14pt;\">\u0634\u0627\u0645\u0644 \u0646\u0645\u0648\u0646\u0647\u200c\u0627\u06cc \u0627\u0633\u062a \u06a9\u0647 \u062d\u062a\u0645\u0627\u064b \u062a\u0648\u0627\u0644\u06cc \u0647\u062f\u0641 \u062f\u0631 \u0622\u0646 \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f.<\/span><\/li>\n<li><span style=\"font-size: 14pt;\">\u0647\u062f\u0641: \u062a\u0623\u06cc\u06cc\u062f \u0627\u06cc\u0646\u06a9\u0647 \u0648\u0627\u06a9\u0646\u0634 PCR \u06cc\u0627 \u0647\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634 \u062f\u06cc\u06af\u0631\u06cc \u0628\u0647 \u062f\u0631\u0633\u062a\u06cc \u06a9\u0627\u0631 \u0645\u06cc\u200c\u06a9\u0646\u062f.<\/span><\/li>\n<li><span style=\"font-size: 14pt;\">\u0627\u06af\u0631 \u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a \u0646\u062a\u06cc\u062c\u0647 \u0645\u0646\u0641\u06cc \u0628\u062f\u0647\u062f\u060c \u06cc\u0639\u0646\u06cc \u0645\u0634\u06a9\u0644\u06cc \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634 \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f.<\/span><\/li>\n<\/ul>\n<ul style=\"text-align: justify;\">\n<li><span style=\"font-size: 14pt;\"><strong>\u06a9\u0646\u062a\u0631\u0644 \u0645\u0646\u0641\u06cc<\/strong><strong> (Negative Control):<\/strong><\/span><\/li>\n<\/ul>\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0634\u0627\u0645\u0644 \u0646\u0645\u0648\u0646\u0647\u200c\u0627\u06cc \u0628\u062f\u0648\u0646 \u062a\u0648\u0627\u0644\u06cc \u0647\u062f\u0641 \u0627\u0633\u062a (\u0645\u062b\u0644\u0627\u064b \u0622\u0628 \u0645\u0642\u0637\u0631 \u06cc\u0627 \u0644\u0648\u0644\u0647 \u062e\u0627\u0644\u06cc).<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0647\u062f\u0641: \u0628\u0631\u0631\u0633\u06cc \u0622\u0644\u0648\u062f\u06af\u06cc \u06cc\u0627 \u062e\u0637\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06af\u0631 \u06a9\u0646\u062a\u0631\u0644 \u0645\u0646\u0641\u06cc \u0645\u062b\u0628\u062a \u0634\u0648\u062f\u060c \u06cc\u0639\u0646\u06cc \u0646\u0645\u0648\u0646\u0647 \u06cc\u0627 \u0645\u062d\u06cc\u0637 \u0622\u0632\u0645\u0627\u06cc\u0634 \u0628\u0647 \u062a\u0648\u0627\u0644\u06cc \u0647\u062f\u0641 \u0622\u0644\u0648\u062f\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a.<\/span><\/li>\n<\/ul>\n[\/vc_message][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><a name=\"_Toc208648552\"><\/a><span style=\"font-size: 18pt;\">3.3. \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644\u200c\u0647\u0627\u06cc \u0633\u0641\u0627\u0631\u0634 \u0648 \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0648\u0627\u06a9\u0646\u0634\u200c\u06af\u0631\u0647\u0627 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0645\u0627\u0645 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u060c \u067e\u0631\u0648\u0628\u200c\u0647\u0627 \u0648 DNA \u06a9\u0646\u062a\u0631\u0644 \u0647\u062f\u0641 \u00a0(Target control DNAs) \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u00a0\u00a0\u0628\u0647 \u0635\u0648\u0631\u062a \u062a\u062c\u0627\u0631\u06cc \u062a\u0648\u0633\u0637 Integrated DNA Technologies (IDT) \u00a0\u062f\u0631 Coralville\u060c \u0627\u06cc\u0627\u0644\u062a \u0622\u06cc\u0648\u0648\u0627\u060c \u0633\u0646\u062a\u0632 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>1.3.3. \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0645\u0627\u0645 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u0627\u0632 IDT \u0633\u0641\u0627\u0631\u0634 \u062f\u0627\u062f\u0647 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f \u0648 \u062f\u0631 \u063a\u0644\u0638\u062a \u06a9\u0627\u0631\u06cc \u06f5\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0645\u0648\u0644\u0627\u0631 \u0646\u0631\u0645\u0627\u0644\u06cc\u0632\u0647 \u0634\u062f\u0647 \u0648 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f2\u06f0- \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u0645\u06cc\u200c\u0634\u0648\u0646\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0633\u0641\u0627\u0631\u0634 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u00a0:<\/span><\/p>\n<ol>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0635\u0641\u062d\u0647 \u0622\u0646\u0644\u0627\u06cc\u0646 \u0633\u0641\u0627\u0631\u0634 IDT\u060c \u06af\u0632\u06cc\u0646\u0647 &#8220;Custom DNA Oligos&#8221; \u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u0632 \u0645\u0646\u0648\u06cc \u06a9\u0634\u0648\u06cc\u06cc &#8220;Normalization&#8221;\u060c \u06af\u0632\u06cc\u0646\u0647 &#8220;Create a custom formulation&#8221; \u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06af\u0632\u06cc\u0646\u0647 &#8220;Full product yield, to a specified \u00b5Molar concentration&#8221; \u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0631\u062f\u0647 \u0648 \u0645\u0642\u062f\u0627\u0631 \u06f5\u06f0\u06f0 \u0631\u0627 \u0648\u0627\u0631\u062f \u06a9\u0646\u06cc\u062f \u0648 IDTE 8.0 pH \u00a0\u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u0646\u0645\u0627\u06cc\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u0627\u0645 \u0646\u0631\u0645\u0627\u0644\u200c\u0633\u0627\u0632\u06cc \u0631\u0627 &#8220;500 \u00b5M&#8221; \u00a0\u06af\u0630\u0627\u0634\u062a\u0647 \u0648 \u0630\u062e\u06cc\u0631\u0647 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u067e\u0633 \u062f\u0631 \u0635\u0641\u062d\u0647 Oligo Entry\u060c \u06af\u0632\u06cc\u0646\u0647\u200c\u0647\u0627\u06cc \u0645\u0631\u0628\u0648\u0637 \u0628\u0647 \u0647\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0631\u0627 \u0645\u0637\u0627\u0628\u0642 \u0645\u0634\u062e\u0635\u0627\u062a \u0647\u0631 \u067e\u0631\u0627\u06cc\u0645\u0631 \u0648\u0627\u0631\u062f \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<\/ol>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h2 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 2. \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0633\u0641\u0627\u0631\u0634 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627<\/span><\/h2>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 49.875%;\" width=\"288\"><span style=\"font-size: 14pt;\"><strong>\u062d\u062c\u0645<\/strong><\/span><\/td>\n<td style=\"width: 50%;\" width=\"288\"><span style=\"font-size: 14pt;\">\u06cc\u06a9 \u0645\u0642\u06cc\u0627\u0633 \u0628\u06cc\u0646 \u06f2\u06f5 \u0646\u0627\u0646\u0648\u0645\u0648\u0644 \u0648 \u06f1 \u0645\u06cc\u06a9\u0631\u0648\u0645\u0648\u0644 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%;\" width=\"288\"><span style=\"font-size: 14pt;\"><strong>\u0646\u0631\u0645\u0627\u0644\u200c\u0633\u0627\u0632\u06cc<\/strong><\/span><\/td>\n<td style=\"width: 50%;\" width=\"288\"><span style=\"font-size: 14pt;\">\u06f5\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0645\u0648\u0644\u0627\u0631 (\u00b5M)<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%;\" width=\"288\"><span style=\"font-size: 14pt;\"><strong>\u062e\u0627\u0644\u0635\u200c\u0633\u0627\u0632\u06cc<\/strong><\/span><\/td>\n<td style=\"width: 50%;\" width=\"288\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0627\u0633\u0627\u0632\u06cc \u0646\u0645\u06a9 \u0628\u0647 \u0631\u0648\u0634 \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 3. \u0646\u0627\u0645 \u0648 \u062a\u0648\u0627\u0644\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631\u200c\u0647\u0627<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%; height: 401px;\">\n<tbody>\n<tr style=\"height: 61px;\">\n<td style=\"width: 33.25%; height: 61px;\" width=\"108\">\n<p style=\"text-align: center;\">\n<\/td>\n<td style=\"width: 20.2083%; height: 61px; text-align: right;\"><span style=\"font-size: 14pt;\"><strong>\u0646\u0627\u0645 \u0622\u06cc\u062a\u0645<\/strong><\/span><\/td>\n<td style=\"width: 46.375%; height: 61px;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u062a\u0648\u0627\u0644\u06cc<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<tr style=\"height: 85px;\">\n<td style=\"width: 33.25%; height: 85px;\" width=\"155\"><span style=\"font-size: 14pt;\">\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc forward \u0628\u0631\u0627\u06cc \u062a\u0642\u0648\u06cc\u062a \u0647\u062f\u0641 \u0645\u06cc\u062a\u0648\u06a9\u0646\u062f\u0631\u06cc\u0627\u06cc\u06cc C. cayetanensis<\/span><\/td>\n<td style=\"width: 20.2083%; height: 85px;\" width=\"108\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">Mit1C-f<\/span><\/p>\n<\/td>\n<td style=\"width: 46.375%;\" width=\"312\">\n<p dir=\"ltr\" style=\"padding-right: 40px; text-align: center;\"><span style=\"font-size: 14pt;\">5&#8242;-TCTATTTTCACCATTCTTGCTCAC-3&#8242;<\/span><\/p>\n<\/td>\n<\/tr>\n<tr style=\"height: 85px;\">\n<td style=\"width: 33.25%; height: 85px;\" width=\"155\"><span style=\"font-size: 14pt;\">\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc reverse \u0628\u0631\u0627\u06cc \u062a\u0642\u0648\u06cc\u062a \u0647\u062f\u0641 \u0645\u06cc\u062a\u0648\u06a9\u0646\u062f\u0631\u06cc\u0627\u06cc\u06cc C.\u202fcayetanensis<\/span><\/td>\n<td style=\"width: 20.2083%; height: 85px;\" width=\"108\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">Mit1C-r<\/span><\/p>\n<\/td>\n<td dir=\"ltr\" style=\"width: 46.375%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">5&#8242;-TGGACTTACTAGGGTGGAGTCT-3&#8242;\u00a0<\/span><\/p>\n<\/td>\n<\/tr>\n<tr style=\"height: 85px;\">\n<td style=\"width: 33.25%; height: 85px;\" width=\"155\"><span style=\"font-size: 14pt;\">\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc forward \u0628\u0631\u0627\u06cc \u062a\u0642\u0648\u06cc\u062a \u0647\u062f\u0641 IAC<\/span><\/td>\n<td style=\"width: 20.2083%; height: 85px;\" width=\"108\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">dd-IAC-f<\/span><\/p>\n<\/td>\n<td dir=\"ltr\" style=\"width: 46.375%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">5&#8242;-CTAACCTTCGTGATGAGCAATCG-3&#8242;<\/span><\/p>\n<\/td>\n<\/tr>\n<tr style=\"height: 85px;\">\n<td style=\"width: 33.25%; height: 85px;\" width=\"155\"><span style=\"font-size: 14pt;\">\u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc reverse \u0628\u0631\u0627\u06cc \u062a\u0642\u0648\u06cc\u062a \u0647\u062f\u0641 IAC<\/span><\/td>\n<td style=\"width: 20.2083%;\" width=\"108\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">dd-IAC-r<\/span><\/p>\n<\/td>\n<td dir=\"ltr\" style=\"width: 46.375%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">5&#8242;-GATCAGCTACGTGAGGTCCTAC-3\u2019<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">4.3. \u067e\u0631\u0648\u0628\u200c\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0627\u0647\u062f\u0627\u0641 C. cayetanensis \u00a0\u00a0\u0648 IAC \u0627\u0632 \u067e\u0631\u0648\u0628\u200c\u0647\u0627\u06cc \u0647\u06cc\u062f\u0631\u0648\u0644\u06cc\u0632 \u0633\u0628\u06a9 Taqman \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u067e\u0631\u0648\u0628 cayetanensis \u0628\u0627 \u0631\u0646\u06af \u06af\u0632\u0627\u0631\u0634\u06af\u0631\u2019\u06f5 FAM \u0646\u0634\u0627\u0646\u0647\u200c\u06af\u0630\u0627\u0631\u06cc \u0634\u062f\u0647 \u0648 \u0628\u0647 \u0635\u0648\u0631\u062a \u062f\u0648\u06af\u0627\u0646\u0647 \u062e\u0627\u0645\u0648\u0634 (quenched) \u00a0\u0627\u0633\u062a: \u06cc\u06a9 \u00a0ZEN \u062f\u0627\u062e\u0644\u06cc \u0648 \u06cc\u06a9 Iowa Black\u00ae FQ (IABkFQ) \u00a0\u062f\u0631 \u0627\u0646\u062a\u0647\u0627\u06cc\u2019\u06f3 \u0622\u0646 \u0642\u0631\u0627\u0631 \u062f\u0627\u0631\u062f.<\/span><\/li>\n<\/ul>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0647 \u0632\u0628\u0627\u0646 \u0633\u0627\u062f\u0647:<\/span><\/p>\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">FAM: \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0647\u0646\u06af\u0627\u0645 \u0627\u062a\u0635\u0627\u0644 \u06cc\u0627 \u0634\u06a9\u0633\u062a \u067e\u0631\u0648\u0628 \u0627\u06cc\u062c\u0627\u062f \u0645\u06cc\u200c\u0634\u0648\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">ZEN \u062f\u0627\u062e\u0644\u06cc \u0648\u00a0 IABkFQ: \u0628\u0627\u0639\u062b \u062e\u0627\u0645\u0648\u0634 \u0634\u062f\u0646 \u0633\u06cc\u06af\u0646\u0627\u0644 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f \u062a\u0627 \u0641\u0642\u0637 \u0647\u0646\u06af\u0627\u0645 \u062a\u0634\u062e\u06cc\u0635 \u062a\u0648\u0627\u0644\u06cc \u0647\u062f\u0641\u060c \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0622\u0632\u0627\u062f \u0634\u0648\u062f \u0648 \u0633\u06cc\u06af\u0646\u0627\u0644 \u062f\u0642\u06cc\u0642 \u0628\u0627\u0634\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u067e\u0631\u0648\u0628 IAC \u0628\u0627 \u0631\u0646\u06af \u06af\u0632\u0627\u0631\u0634\u06af\u0631\u2019 \u06f5 Cy5 \u0646\u0634\u0627\u0646\u0647\u200c\u06af\u0630\u0627\u0631\u06cc \u0634\u062f\u0647 \u0648 \u0628\u0647 \u0635\u0648\u0631\u062a \u062f\u0648\u06af\u0627\u0646\u0647 \u062e\u0627\u0645\u0648\u0634 (quenched) \u0627\u0633\u062a: \u06cc\u06a9 \u00a0TAO \u062f\u0627\u062e\u0644\u06cc \u0648 \u06cc\u06a9 \u00a0Iowa Black\u00ae RQ-Sp (IAbRQSp) \u062f\u0631 \u0627\u0646\u062a\u0647\u0627\u06cc\u2019\u06f3 \u0622\u0646 \u0642\u0631\u0627\u0631 \u062f\u0627\u0631\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0645\u0627\u0645 \u067e\u0631\u0648\u0628\u200c\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u00a0\u0627\u0632 IDT \u00a0\u0633\u0641\u0627\u0631\u0634 \u062f\u0627\u062f\u0647 \u0634\u062f\u0647\u200c\u0627\u0646\u062f \u0648 \u0637\u0628\u0642 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644\u060c \u0628\u0631\u0627\u06cc \u0631\u0633\u06cc\u062f\u0646 \u0628\u0647 \u063a\u0644\u0638\u062a \u06a9\u0627\u0631\u06cc \u0647\u06cc\u062f\u0631\u0627\u062a\u0647 \u0634\u062f\u0647 \u0648 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f2\u06f0- \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u0645\u06cc\u200c\u0634\u0648\u0646\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4 style=\"text-align: justify;\">1.4.3. \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0633\u0641\u0627\u0631\u0634 \u067e\u0631\u0648\u0628\u200c\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0635\u0641\u062d\u0647 \u0622\u0646\u0644\u0627\u06cc\u0646 \u0633\u0641\u0627\u0631\u0634 IDT\u060c \u06af\u0632\u06cc\u0646\u0647 &#8220;Custom qPCR Probes&#8221; \u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u067e\u0633 \u00a0PrimeTime qPCR Probes \u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u0642\u06cc\u0627\u0633 \u06f2\u06f5\u06f0 \u0646\u0627\u0646\u0648\u0645\u0648\u0644 \u06cc\u0627 \u06f1 \u0645\u06cc\u06a9\u0631\u0648\u0645\u0648\u0644 \u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0648\u0627\u0644\u06cc \u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u06cc \u067e\u0631\u0648\u0628 \u0631\u0627 \u0648\u0627\u0631\u062f \u06a9\u0631\u062f\u0647 \u0648 \u06af\u0632\u06cc\u0646\u0647\u200c\u0647\u0627\u06cc &#8221; \u2019\u06f5\u00a0Dye \/ \u06f3\u2019 Quencher&#8221; \u0631\u0627 \u0645\u0637\u0627\u0628\u0642 \u062c\u062f\u0648\u0644 4 \u0628\u0631\u0627\u06cc \u0647\u0631 \u067e\u0631\u0648\u0628 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0647\u06cc\u0686 \u06af\u0632\u06cc\u0646\u0647\u200c\u0627\u06cc \u062a\u062d\u062a \u0628\u062e\u0634 &#8220;Services&#8221; \u0644\u0627\u0632\u0645 \u0646\u06cc\u0633\u062a \u0627\u0646\u062a\u062e\u0627\u0628 \u0634\u0648\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 4: \u0627\u0637\u0644\u0627\u0639\u0627\u062a \u0633\u0641\u0627\u0631\u0634 \u067e\u0631\u0648\u0628<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 1.25%;\"><\/td>\n<td style=\"width: 46.4583%;\" width=\"275\"><span style=\"font-size: 14pt;\"><strong>\u067e\u0631\u0648\u0628 \u0628\u0631\u0627\u06cc \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0647\u062f\u0641<\/strong> IAC<\/span><\/td>\n<td style=\"width: 52.125%;\" width=\"247\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u067e\u0631\u0648\u0628 \u0628\u0631\u0627\u06cc \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0647\u062f\u0641<\/strong> C. cayetanensis<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 1.25%;\" width=\"91\"><span style=\"font-size: 14pt;\"><strong>\u0646\u0627\u0645 \u0622\u06cc\u062a\u0645<\/strong><\/span><\/td>\n<td style=\"width: 46.4583%;\" width=\"275\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">dd-IAC-Cy5<\/span><\/p>\n<\/td>\n<td style=\"width: 52.125%;\" width=\"247\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">Mit1P-FAM<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 1.25%;\" width=\"91\"><span style=\"font-size: 14pt;\"><strong>\u062a\u0648\u0627\u0644\u06cc<\/strong><\/span><\/td>\n<td style=\"width: 46.4583%;\">\n<p dir=\"ltr\"><span style=\"font-size: 14pt;\">5&#8217;AGCTAGTCGATGCACTCCAGTCCTCCT-3&#8242;<\/span><\/p>\n<p>&nbsp;<\/td>\n<td style=\"width: 52.125%;\">\n<p dir=\"ltr\" style=\"text-align: center;\"><span style=\"font-size: 14pt;\">5&#8242;-AGGAGATAGAATGCTGGTGTATGCACC -3&#8242;<\/span><\/p>\n<p dir=\"ltr\">\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 1.25%;\" width=\"91\"><span style=\"font-size: 14pt;\"><strong>\u06a9\u064f\u062f\u0650 \u0646\u0627\u062d\u06cc\u0647 5 \u067e\u0631\u0627\u06cc\u0645<\/strong><\/span><\/td>\n<td style=\"width: 46.4583%;\" width=\"275\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\/Cy5\/\u00a0<\/span><\/p>\n<\/td>\n<td style=\"width: 52.125%;\" width=\"247\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\/56-FAM\/<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 1.25%;\" width=\"91\"><span style=\"font-size: 14pt;\"><strong>\u062e\u0627\u0645\u0648\u0634\u200c\u06a9\u0646\u0646\u062f\u0647 \u062f\u0631 \u0627\u0646\u062a\u0647\u0627\u06cc 3 \u067e\u0631\u0627\u06cc\u0645<\/strong><\/span><\/td>\n<td style=\"width: 46.4583%;\" width=\"275\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">TAO-3\u2019 Iowa Black\u00ae\u00a0RQ-Sp<\/span><\/p>\n<\/td>\n<td style=\"width: 52.125%;\" width=\"247\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">ZEN-3\u2019 Iowa Black\u00ae\u00a0FQ\u00a0<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 1.25%;\" width=\"91\"><span style=\"font-size: 14pt;\"><strong>\u06a9\u064f\u062f\u0650 \u0627\u0646\u062a\u0647\u0627\u06cc 3 \u067e\u0631\u0627\u06cc\u0645<\/strong><\/span><\/td>\n<td style=\"width: 46.4583%;\" width=\"275\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\/3IAbRQSp\/\u00a0<\/span><\/p>\n<\/td>\n<td style=\"width: 52.125%;\" width=\"247\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\/3IABkFQ\/<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><span style=\"font-size: 18pt;\">5.3. \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0645\u062d\u0644\u0648\u0644\u200c\u0647\u0627\u06cc \u06a9\u0627\u0631\u06cc \u067e\u0631\u0648\u0628\u200c\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u00a0<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">100\u00b5M Mit1P-FAM: \u067e\u0631\u0648\u0628 \u062e\u0634\u06a9 (lyophilized) \u0631\u0627 \u062f\u0631 \u00a0TE buffer \u0627\u0633\u062a\u0631\u06cc\u0644 \u0648 \u0641\u0627\u0642\u062f DNase \u0647\u06cc\u062f\u0631\u0627\u062a\u0647 \u06a9\u0646\u06cc\u062f \u0648 \u062d\u062c\u0645 \u0645\u0648\u0631\u062f \u0646\u06cc\u0627\u0632 \u0628\u0631\u0627\u06cc \u0631\u0633\u06cc\u062f\u0646 \u0628\u0647 \u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc \u06f1\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0645\u0648\u0644\u0627\u0631 \u0631\u0627 \u0637\u0628\u0642 \u0628\u0631\u06af\u0647 \u0645\u0634\u062e\u0635\u0627\u062a \u067e\u0631\u0648\u0628 IDT \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0645\u062d\u0644\u0648\u0644 \u0631\u0627 \u0648\u0631\u062a\u06a9\u0633 \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0647\u200c\u0637\u0648\u0631 \u06a9\u0648\u062a\u0627\u0647 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u00a0100\u00b5M dd-IAC-Cy5: \u067e\u0631\u0648\u0628 \u062e\u0634\u06a9 \u0631\u0627 \u062f\u0631 \u00a0TE buffer \u0627\u0633\u062a\u0631\u06cc\u0644 \u0648 \u0641\u0627\u0642\u062f DNase \u0647\u06cc\u062f\u0631\u0627\u062a\u0647 \u06a9\u0646\u06cc\u062f \u0648 \u062d\u062c\u0645 \u0645\u0648\u0631\u062f \u0646\u06cc\u0627\u0632 \u0628\u0631\u0627\u06cc \u0631\u0633\u06cc\u062f\u0646 \u0628\u0647 \u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc \u06f1\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0645\u0648\u0644\u0627\u0631 \u0631\u0627 \u0637\u0628\u0642 \u0628\u0631\u06af\u0647 \u0645\u0634\u062e\u0635\u0627\u062a \u067e\u0631\u0648\u0628 IDT \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0645\u062d\u0644\u0648\u0644 \u0631\u0627 \u0648\u0631\u062a\u06a9\u0633 \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0647 \u0645\u062f\u062a \u06a9\u0648\u062a\u0627\u0647 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><span style=\"font-size: 18pt;\">6.3. \u0647\u062f\u0641 \u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc \u062a\u0642\u0648\u06cc\u062a (IAC Target) \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u00a0<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0647\u062f\u0641 \u0648\u0627\u06a9\u0646\u0634 IAC (HMultra130-synIAC) \u06cc\u06a9 \u062a\u0648\u0627\u0644\u06cc DNA \u0645\u0635\u0646\u0648\u0639\u06cc \u06f2\u06f0\u06f0 \u062c\u0641\u062a \u0628\u0627\u0632 (bp) \u0627\u0633\u062a \u06a9\u0647 \u0628\u0631 \u0627\u0633\u0627\u0633 \u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc \u062a\u0642\u0648\u06cc\u062a \u062a\u0648\u0633\u0639\u0647\u200c\u06cc\u0627\u0641\u062a\u0647 \u062a\u0648\u0633\u0637 \u00a0Deer \u0648 \u0647\u0645\u06a9\u0627\u0631\u0627\u0646\u060c \u062f\u0631 \u0633\u0627\u0644 \u06f2\u06f0\u06f1\u06f0 \u0633\u0627\u062e\u062a\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>1.6.3. \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0633\u0641\u0627\u0631\u0634:<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0635\u0641\u062d\u0647 \u0633\u0641\u0627\u0631\u0634 \u0622\u0646\u0644\u0627\u06cc\u0646 IDT\u060c \u06af\u0632\u06cc\u0646\u0647 &#8220;Ultramer Oligos (up to 200 bases)&#8221; \u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0635\u0641\u062d\u0647 Oligo Entry\u060c \u06af\u0632\u06cc\u0646\u0647\u200c\u0647\u0627\u06cc \u0645\u0631\u0628\u0648\u0637\u0647 \u0631\u0627 \u0648\u0627\u0631\u062f \u06cc\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f (\u062c\u0632\u0626\u06cc\u0627\u062a \u062f\u0642\u06cc\u0642 \u062a\u0648\u0627\u0644\u06cc \u0648 \u0645\u0634\u062e\u0635\u0627\u062a \u0647\u0631 IAC \u062f\u0631 \u0627\u062f\u0627\u0645\u0647 \u062c\u062f\u0648\u0644 \u06cc\u0627 \u0645\u062a\u0646 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0627\u0631\u0627\u0626\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a).<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 5. \u062a\u0648\u0636\u06cc\u062d\u0627\u062a \u0627\u0644\u06cc\u06af\u0648\u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f\u0647\u0627\u06cc Ultramer<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%; height: 175px;\">\n<tbody>\n<tr style=\"height: 61px;\">\n<td style=\"width: 49.875%; height: 61px;\" width=\"287\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u0646\u0627\u0645 \u0622\u06cc\u062a\u0645<\/strong><\/span><\/p>\n<\/td>\n<td style=\"width: 50%;\" width=\"288\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">HMultra130-synIAC<\/span><\/p>\n<\/td>\n<\/tr>\n<tr style=\"height: 38px;\">\n<td style=\"width: 49.875%; height: 38px; text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u062d\u062c\u0645<\/strong><\/span><\/td>\n<td style=\"width: 50%;\" width=\"288\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u0627\u0644\u06cc\u06af\u0648\u0646\u0648\u06a9\u0644\u0626\u0648\u062a\u06cc\u062f DNA Ultramer\u2122 \u0628\u0627 \u0645\u0642\u062f\u0627\u0631 \u06f4 \u0646\u0627\u0646\u0648\u0645\u0648\u0644 (4 nmole)<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%;\" width=\"287\">\n<p style=\"text-align: right;\"><span style=\"font-size: 14pt;\"><strong>\u0646\u0631\u0645\u0627\u0644\u200c\u0633\u0627\u0632\u06cc<\/strong><\/span><\/p>\n<\/td>\n<td style=\"width: 50%; height: 38px; text-align: center;\"><span style=\"font-size: 14pt;\">\u0646\u062f\u0627\u0631\u062f<\/span><\/td>\n<\/tr>\n<tr style=\"height: 38px;\">\n<td style=\"width: 49.875%; height: 38px; text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u062e\u0627\u0644\u0635\u200c\u0633\u0627\u0632\u06cc<\/strong><\/span><\/td>\n<td style=\"width: 50%;\" width=\"288\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062a\u0635\u0641\u06cc\u0647 \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f \u00a0(Standard Desalting)<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p dir=\"ltr\" style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">TACAGCACCCTAGCTTGGTAGAATCGATCAGCTACGTGAGGTCCTACGACGATCGCCAAGCATGCCCTAGCTAAGATGCATCGATTGCTCATCACGTACGTTAGGTCGACTAGGAGGACTGGAGTGCATCGACTAGCTAAGATGGTTCGATTGCTCATCACGAAGGTTAGGTCGACTACGAACGAGTCGTATTGCAGGTT\u00a0<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">7.3. \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0645\u062d\u0644\u0648\u0644 \u06a9\u0627\u0631\u06cc \u0647\u062f\u0641 IAC<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0622\u0644\u062a\u0631\u0627\u0645\u0631 \u0647\u062f\u0641 IAC (HMultra130-synIAC) \u0631\u0627 \u0645\u0637\u0627\u0628\u0642 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644\u200c\u0647\u0627\u06cc \u0632\u06cc\u0631 \u062f\u0631 \u0628\u0627\u0641\u0631 \u0631\u0642\u06cc\u0642\u200c\u0633\u0627\u0632\u06cc TE \u0628\u0627 pH 7.5 \u00a0\u0647\u06cc\u062f\u0631\u0627\u062a\u0647 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0628\u0647 \u063a\u0644\u0638\u062a \u06a9\u0627\u0631\u06cc \u06f1\u06f0<sup>7<\/sup>\u00d7 1 \u00b5L\/\u0646\u0633\u062e\u0647 \u0628\u0631\u0633\u06cc\u062f. \u0645\u062d\u0644\u0648\u0644\u200c\u0647\u0627\u06cc \u0631\u0642\u06cc\u0642\u200c\u0634\u062f\u0647 \u0648 \u0645\u062d\u0644\u0648\u0644 \u06a9\u0627\u0631\u06cc \u0631\u0627 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f2\u06f0 -\u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 6. \u0645\u062d\u0644\u0648\u0644 \u06a9\u0627\u0631\u06cc \u0647\u062f\u0641 \u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc \u062a\u0642\u0648\u06cc\u062a(IAC)<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%; height: 621px;\">\n<tbody>\n<tr style=\"height: 70px;\">\n<td dir=\"ltr\" style=\"width: 49.875%; height: 70px;\" width=\"300\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">(<strong>\u0646\u0633\u062e\u0647\u200c\u0647\u0627<\/strong>\/\u00b5L)<strong> \u063a\u0644\u0638\u062a<\/strong><\/span><\/p>\n<\/td>\n<td style=\"width: 50%; text-align: center; height: 70px;\" width=\"299\"><span style=\"font-size: 14pt;\">HMultra130-synIAC <strong>\u0647\u062f\u0641<\/strong> IAC: <strong>\u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0647\u06cc\u062f\u0631\u0627\u062a\u0627\u0633\u06cc\u0648\u0646 \u0648 \u0631\u0642\u06cc\u0642\u200c\u0633\u0627\u0632\u06cc<\/strong><\/span><\/td>\n<\/tr>\n<tr style=\"height: 203px;\">\n<td style=\"width: 49.875%; height: 203px;\" width=\"300\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">5E12<\/span><\/p>\n<\/td>\n<td style=\"width: 50%; height: 203px;\" width=\"299\"><span style=\"font-size: 14pt;\">\u0642\u0628\u0644 \u0627\u0632 \u0628\u0627\u0632 \u06a9\u0631\u062f\u0646\u060c \u0622\u0644\u062a\u0631\u0627\u0645\u0631 \u062e\u0634\u06a9\u200c\u0634\u062f\u0647 (\u06f4 \u0646\u0627\u0646\u0648\u0645\u0648\u0644) \u0631\u0627 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u062d\u062a\u0648\u06cc\u0627\u062a \u062f\u0631 \u062a\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u0646\u062f. \u0622\u0646 \u0631\u0627 \u062f\u0631 \u0644\u0648\u0644\u0647 \u0627\u0635\u0644\u06cc \u0628\u0627 \u06f1\u06f0\u06f0\u06f0 \u00b5L \u0628\u0627\u0641\u0631 \u0631\u0642\u06cc\u0642\u200c\u0633\u0627\u0632\u06cc TE \u0647\u06cc\u062f\u0631\u0627\u062a\u0647 \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0631\u0627\u06cc \u0645\u062f\u062a \u06a9\u0648\u062a\u0627\u0647\u06cc \u0648\u0631\u062a\u06a9\u0633 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u062f\u0648\u0628\u0627\u0631\u0647 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u0627\u06cc\u0639 \u062f\u0631 \u062a\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<tr style=\"height: 98px;\">\n<td style=\"width: 49.875%; height: 98px; text-align: right;\"><span style=\"font-size: 14pt;\">5E10<\/span><\/td>\n<td style=\"width: 50%; height: 98px;\" width=\"299\"><span style=\"font-size: 14pt;\">\u06f1\u06f0 \u00b5L \u0627\u0632 \u0645\u062d\u0644\u0648\u0644 \u0630\u062e\u06cc\u0631\u0647 5E12 \u0631\u0627 \u0628\u0627 \u06f9\u06f9\u06f0 \u00b5L TE \u062f\u0631 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u062f\u06cc\u062f \u0645\u062e\u0644\u0648\u0637 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u0627\u06cc\u0639 \u062f\u0631 \u062a\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<tr style=\"height: 127px;\">\n<td style=\"width: 49.875%; height: 127px; text-align: right;\"><span style=\"font-size: 14pt;\">5E8<\/span><\/td>\n<td style=\"width: 50%; height: 127px;\" width=\"299\"><span style=\"font-size: 14pt;\">\u06f1\u06f0 \u00b5L \u0627\u0632 \u0645\u062d\u0644\u0648\u0644 \u0630\u062e\u06cc\u0631\u0647 5E10 \u0631\u0627 \u0628\u0627 \u06f9\u06f9\u06f0 \u00b5L TE \u062f\u0631 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u062f\u06cc\u062f \u0645\u062e\u0644\u0648\u0637 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u0627\u06cc\u0639 \u062f\u0631 \u062a\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<tr style=\"height: 123px;\">\n<td style=\"width: 49.875%; height: 123px;\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">1E7<\/span><\/p>\n<\/td>\n<td style=\"width: 50%; height: 123px;\" width=\"299\"><span style=\"font-size: 14pt;\">\u06f1\u06f0 \u00b5L \u0627\u0632 \u0645\u062d\u0644\u0648\u0644 \u0630\u062e\u06cc\u0631\u0647 5E8 \u0631\u0627 \u0628\u0627 \u06f4\u06f9\u06f0 \u00b5L TE \u062f\u0631 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u062f\u06cc\u062f \u0645\u062e\u0644\u0648\u0637 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u0627\u06cc\u0639 \u062f\u0631 \u062a\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0648\u062c\u0647: \u0627\u06cc\u0646 \u0645\u0642\u062f\u0627\u0631 \u0628\u0647\u200c\u0635\u0648\u0631\u062a \u062a\u0626\u0648\u0631\u06cc \u0645\u062d\u0627\u0633\u0628\u0647 \u0634\u062f\u0647 \u0648 \u062a\u0627\u0628\u0639\u06cc \u0627\u0632 \u063a\u0644\u0638\u062a \u00a0DNA\u060c \u0639\u062f\u062f \u0622\u0648\u0648\u06af\u0627\u062f\u0631\u0648\u060c \u0637\u0648\u0644 \u0642\u0627\u0644\u0628 \u0648 \u0648\u0632\u0646 \u0645\u062a\u0648\u0633\u0637 \u06cc\u06a9 \u062c\u0641\u062a \u0628\u0627\u0632 \u0627\u0633\u062a. \u0627\u0628\u0632\u0627\u0631\u0647\u0627\u06cc \u0622\u0646\u0644\u0627\u06cc\u0646 \u0631\u0627\u06cc\u06af\u0627\u0646\u06cc \u0628\u0631\u0627\u06cc \u0645\u062d\u0627\u0633\u0628\u0647 \u0627\u06cc\u0646 \u0645\u0642\u062f\u0627\u0631 \u062f\u0631 \u062f\u0633\u062a\u0631\u0633 \u0647\u0633\u062a\u0646\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<h4>\u00a0<strong data-start=\"72\" data-end=\"123\">\u0645\u062d\u0627\u0633\u0628\u0647 \u062a\u0639\u062f\u0627\u062f \u0646\u0633\u062e\u0647\u200c\u0647\u0627\u06cc DNA \u0647\u062f\u0641<\/strong><\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u0631\u062d\u0644\u0647 \u06f1: \u0686\u0647\u0627\u0631 \u0646\u0627\u0646\u0648\u0645\u0648\u0644 \u0631\u0627 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u0645\u0627\u0634\u06cc\u0646\u200c\u062d\u0633\u0627\u0628 \u062a\u0628\u062f\u06cc\u0644 \u0645\u0642\u062f\u0627\u0631 \u0645\u0648\u0644\u06cc \u0628\u0647 \u0648\u0632\u0646\u060c \u0628\u0631\u0627\u06cc \u0627\u0633\u06cc\u062f\u0647\u0627\u06cc \u0646\u0648\u06a9\u0644\u0626\u06cc\u06a9 \u0628\u0647 \u0645\u06cc\u06a9\u0631\u0648\u06af\u0631\u0645 DNA \u062a\u0628\u062f\u06cc\u0644 \u06a9\u0646\u06cc\u062f. \u0648\u0632\u0646 \u0645\u062d\u0627\u0633\u0628\u0647\u200c\u0634\u062f\u0647 \u0628\u0631\u0627\u0628\u0631 \u0628\u0627 \u06f5\u06f1\u06f9.\u06f2 \u0645\u06cc\u06a9\u0631\u0648\u06af\u0631\u0645 \u0627\u0633\u062a.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u0631\u062d\u0644\u0647 \u06f2: \u0627\u0632 \u0648\u0632\u0646 \u0645\u062d\u0627\u0633\u0628\u0647\u200c\u0634\u062f\u0647 DNA (\u06f5\u06f1\u06f9.\u06f2 \u0645\u06cc\u06a9\u0631\u0648\u06af\u0631\u0645) \u0648 \u0648\u0632\u0646 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u062a\u0648\u0627\u0644\u06cc (\u06f6\u06f1\u06f8\u06f8\u06f2) \u0628\u0631\u0627\u06cc \u0645\u062d\u0627\u0633\u0628\u0647 \u062a\u0639\u062f\u0627\u062f \u0646\u0633\u062e\u0647\u200c\u0647\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u06a9\u0646\u06cc\u062f. (\u0627\u06cc\u0646 \u0645\u0642\u062f\u0627\u0631 \u062a\u0642\u0631\u06cc\u0628\u0627\u064b 10<sup>15<\/sup>\u00d7 \u06f5.\u06f1\u06f3\u06f8 \u0646\u0633\u062e\u0647 \u062e\u0648\u0627\u0647\u062f \u0628\u0648\u062f ) ( \u0628\u0627 \u0641\u0631\u0636 \u062c\u0631\u0645 \u0647\u0631 \u062c\u0641\u062a \u0628\u0627\u0632 DNA \u0628\u0631\u0627\u0628\u0631 \u06f6\u06f5\u06f0 \u062f\u0627\u0644\u062a\u0648\u0646). \u0627\u0632 \u0622\u0646\u062c\u0627 \u06a9\u0647 \u0647\u062f\u0641 \u062e\u0634\u06a9\u200c\u0634\u062f\u0647 \u062f\u0631 TE \u00a0\u0628\u0627 \u063a\u0644\u0638\u062a \u00b5L \u00a0\u06f1\u06f0\u06f0\u06f0 \u062d\u0644 \u0634\u062f\u0647 \u0627\u0633\u062a\u060c \u062a\u0639\u062f\u0627\u062f \u062a\u0642\u0631\u06cc\u0628\u06cc \u0646\u0633\u062e\u0647\u200c\u0647\u0627 \u0628\u0631\u0627\u0628\u0631 \u06f1\u06f0<sup>12<\/sup>\u00d7\u06f5 \u00b5L \/\u0646\u0633\u062e\u0647 \u062e\u0648\u0627\u0647\u062f \u0628\u0648\u062f.)<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0648\u062c\u0647: \u06cc\u06a9 \u0631\u0648\u0634 \u0627\u062e\u062a\u06cc\u0627\u0631\u06cc \u0628\u0631\u0627\u06cc \u0627\u0646\u062f\u0627\u0632\u0647\u200c\u06af\u06cc\u0631\u06cc \u062f\u0642\u06cc\u0642 \u00a0DNA \u0647\u062f\u0641 \u0627\u06cc\u0646 \u0627\u0633\u062a \u06a9\u0647 DNA \u062e\u0634\u06a9\u200c\u0634\u062f\u0647 \u0631\u0627 \u062f\u0631 \u06f1\u06f0\u06f0\u06f0 \u00b5L \u0645\u062d\u0644\u0648\u0644 TE \u062d\u0644 \u06a9\u0646\u06cc\u0645.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u067e\u0633 \u063a\u0644\u0638\u062a DNA \u0631\u0627 \u0628\u0627 \u06a9\u06cc\u062a Qubit \u062d\u0633\u0627\u0633\u06cc\u062a \u0628\u0627\u0644\u0627 \u06cc\u0627 \u062f\u0633\u062a\u06af\u0627\u0647 Nanodrop \u0627\u0646\u062f\u0627\u0632\u0647\u200c\u06af\u06cc\u0631\u06cc \u0645\u06cc\u200c\u06a9\u0646\u06cc\u0645 (\u0648\u0627\u062d\u062f: ng\/\u00b5L). \u063a\u0644\u0638\u062a \u0628\u0647 \u062f\u0633\u062a \u0622\u0645\u062f\u0647 \u0631\u0627 \u062f\u0631 \u0645\u0627\u0634\u06cc\u0646\u200c\u062d\u0633\u0627\u0628 \u062a\u0639\u062f\u0627\u062f \u0646\u0633\u062e\u0647\u200c\u0647\u0627 \u0648\u0627\u0631\u062f \u0645\u06cc\u200c\u06a9\u0646\u06cc\u0645 \u062a\u0627 \u062a\u0639\u062f\u0627\u062f \u062f\u0642\u06cc\u0642 \u0646\u0633\u062e\u0647\u200c\u0647\u0627 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0645\u0634\u062e\u0635 \u0634\u0648\u062f. \u062f\u0631 \u0646\u0647\u0627\u06cc\u062a\u060c \u0646\u0645\u0648\u0646\u0647 \u0631\u0627 \u0631\u0642\u06cc\u0642 \u0645\u06cc\u200c\u06a9\u0646\u06cc\u0645 \u062a\u0627 \u0628\u0647 \u06f1\u06f0<sup>7<\/sup>\u00d7 \u06f1 \u00b5L\/\u0646\u0633\u062e\u0647 \u0628\u0631\u0633\u062f. \u0631\u0642\u062a\u200c\u0647\u0627 \u0631\u0627 \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f2\u06f0 <strong>&#8211;<\/strong>\u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">8.3. \u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">DNA \u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a (Mit1AA gblock) \u06cc\u06a9 \u0642\u0637\u0639\u0647 \u0698\u0646\u06cc \u0645\u0635\u0646\u0648\u0639\u06cc bp \u06f2\u06f4\u06f5 \u062f\u0648 \u0631\u0634\u062a\u0647\u200c\u0627\u06cc \u0627\u0633\u062a \u06a9\u0647 \u062a\u0648\u0633\u0637 IDT \u0633\u0646\u062a\u0632 \u0634\u062f\u0647 \u0627\u0633\u062a. \u0627\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0645\u0631\u0628\u0648\u0637 \u0628\u0647 \u0645\u0646\u0637\u0642\u0647 \u06f4\u06f3\u06f2\u06f5 \u062a\u0627 \u06f4\u06f5\u06f6\u06f9 bp \u060c \u062f\u0631 \u0698\u0646 \u0645\u06cc\u062a\u0648\u06a9\u0646\u062f\u0631\u06cc C. cayetanensis \u00a0\u0627\u0633\u062a (Genbank: KP231180,1). \u0639\u0644\u0627\u0648\u0647 \u0628\u0631 \u0627\u06cc\u0646\u060c \u0627\u06cc\u0646 \u062a\u0648\u0627\u0644\u06cc \u0634\u0627\u0645\u0644 \u062c\u0647\u0634\u200c\u0647\u0627\u06cc \u0642\u0627\u0628\u0644 \u0631\u062f\u06cc\u0627\u0628\u06cc (T4385A \u0648 T4386A)\u00a0 \u062f\u0631 \u0622\u0645\u067e\u0644\u06cc\u06a9\u0648\u0646\u06cc \u0627\u0633\u062a \u06a9\u0647 \u062a\u0648\u0633\u0637 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627\u06cc PCR \u062f\u0631 \u0632\u0645\u0627\u0646 \u0648\u0627\u0642\u0639\u06cc \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0645\u06cc\u200c\u0634\u0648\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>1.8.3. \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0633\u0641\u0627\u0631\u0634:<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0635\u0641\u062d\u0647 \u0633\u0641\u0627\u0631\u0634 \u0622\u0646\u0644\u0627\u06cc\u0646 IDT\u060c \u06af\u0632\u06cc\u0646\u0647 &#8220;gBlocks Gene Fragments&#8221; \u0631\u0627 \u0627\u0646\u062a\u062e\u0627\u0628 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u0627\u0645 \u0645\u0648\u0631\u062f \u0648 \u062a\u0648\u0627\u0644\u06cc \u0631\u0627 \u062f\u0631 \u0635\u0641\u062d\u0647 \u00a0gBlocks\u00ae Gene Fragments Entry \u0648\u0627\u0631\u062f \u06a9\u0646\u06cc\u062f:<\/span><\/li>\n<\/ul>\n<p dir=\"ltr\" style=\"text-align: right;\"><span style=\"font-size: 14pt;\">\u00a0Mit1AA gblock :\u0646\u0627\u0645 \u0622\u06cc\u062a\u0645<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0648\u0627\u0644\u06cc (Sequence):<\/span><\/li>\n<\/ul>\n<p dir=\"ltr\" style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">ACAGTTGGTTTTCTATTTTCACCATTCTTGCTCACTGTATTAGTATTATTTAATTTTACTAATAGAGAAGTTGGTACTACATCAGCTTCTCTGGTTTCATCAATTTGTTTAGGTGTTATTAGTACTGAGTTACTACTATTTGTTAGCTTCTTCTGGGGTGCATACACCAGCATTCTATCTCCTAGTTATGTAACAGACTCCACCCTAGTAAGTCCAACTGAGGGTCTTGTAAGTATCTCTAGTAG<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0631\u0648\u06cc Add to Order \u06a9\u0644\u06cc\u06a9 \u06a9\u0646\u06cc\u062f \u0648 \u0633\u067e\u0633 \u00a0\u062f\u0631 \u067e\u0646\u062c\u0631\u0647 \u0628\u0627\u0632\u0634\u062f\u0647 Terms and Disclosure\u060c \u0628\u0647 \u0647\u0645\u0647 \u067e\u0631\u0633\u0634\u200c\u0647\u0627 \u067e\u0627\u0633\u062e &#8220;No&#8221; \u0628\u062f\u0647\u06cc\u062f. \u00a0\u0646\u0627\u0645 \u062e\u0648\u062f \u0631\u0627 \u062f\u0631 \u06a9\u0627\u062f\u0631 \u00a0Signature \u0648\u0627\u0631\u062f \u06a9\u0631\u062f\u0647 \u0648 \u0634\u0631\u0627\u06cc\u0637 \u0648 \u0636\u0648\u0627\u0628\u0637 (terms and conditions) \u0631\u0627 \u0628\u067e\u0630\u06cc\u0631\u06cc\u062f . \u0633\u067e\u0633 \u0631\u0648\u06cc &#8220;Add to Cart&#8221; \u06a9\u0644\u06cc\u06a9 \u06a9\u0646\u06cc\u062f. \u0645\u0642\u062f\u0627\u0631 \u062a\u062d\u0648\u06cc\u0644\u06cc \u0627\u0632 \u00a0gBlock \u0628\u0631\u0627\u0628\u0631 \u0628\u0627 \u06f2\u06f5\u06f0 \u0646\u0627\u0646\u0648\u06af\u0631\u0645 \u062e\u0648\u0627\u0647\u062f \u0628\u0648\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">9.3. \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0645\u062d\u0644\u0648\u0644 \u06a9\u0627\u0631\u06cc \u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a gBlock (Mit1AA) \u0631\u0627 \u062f\u0631 \u0628\u0627\u0641\u0631 \u0631\u0642\u06cc\u0642\u200c\u06a9\u0646\u0646\u062f\u0647 TE \u0628\u0627 pH \u0628\u0631\u0627\u0628\u0631 7.5\u060c \u0637\u0628\u0642 \u062f\u0633\u062a\u0648\u0631\u0627\u0644\u0639\u0645\u0644 \u0632\u06cc\u0631 \u0647\u06cc\u062f\u0631\u0627\u062a\u0647 \u0648 \u0631\u0642\u06cc\u0642 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u063a\u0644\u0638\u062a \u06a9\u0627\u0631\u06cc \u0628\u0631\u0627\u0628\u0631 \u0628\u0627 \u200e5E2\u200e \u0646\u0633\u062e\u0647 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0628\u0647 \u062f\u0633\u062a \u0622\u06cc\u062f. \u0631\u0642\u06cc\u0642\u200c\u0633\u0627\u0632\u06cc\u200c\u0647\u0627 \u0631\u0627 \u062f\u0631 \u062f\u0645\u0627\u06cc \u200e20 -\u062f\u0631\u062c\u0647 \u0633\u0644\u0633\u06cc\u0648\u0633 \u0630\u062e\u06cc\u0631\u0647 \u06a9\u0646\u06cc\u062f. \u0645\u062d\u0644\u0648\u0644 \u06a9\u0627\u0631\u06cc \u0631\u0627 \u0645\u06cc\u200c\u062a\u0648\u0627\u0646 \u062f\u0631 \u062f\u0645\u0627\u06cc20 -\u06cc\u0627 4 \u062f\u0631\u062c\u0647 \u0633\u0644\u0633\u06cc\u0648\u0633 \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u06a9\u0631\u062f. \u0647\u0631 \u06f9\u06f0 \u0631\u0648\u0632 \u06cc\u06a9 \u0628\u0627\u0631 \u0628\u0627\u06cc\u062f \u0645\u062d\u0644\u0648\u0644 \u06a9\u0627\u0631\u06cc \u062a\u0627\u0632\u0647 \u0627\u0632 \u0631\u0642\u062a \u200e5E3\u200e \u06cc\u062e\u200c\u0632\u062f\u0647 \u062a\u0647\u06cc\u0647 \u0634\u0648\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 7.\u0645\u062d\u0644\u0648\u0644 \u06a9\u0627\u0631\u06cc \u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a (\u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633)<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\"><strong>\u063a\u0644\u0638\u062a (\u0646\u0633\u062e\u0647 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631)<\/strong><\/span><\/td>\n<td style=\"width: 50%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\"><strong>\u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a<\/strong><strong> Mit1AA: <\/strong><strong>\u0631\u0648\u0634 \u0647\u06cc\u062f\u0631\u0627\u062a\u0647\u200c\u0633\u0627\u0632\u06cc \u0648 \u0631\u0642\u06cc\u0642\u200c\u0633\u0627\u0632\u06cc<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">2E9<\/span><\/p>\n<\/td>\n<td style=\"width: 50%;\" width=\"312\"><span style=\"font-size: 14pt;\">\u0642\u0628\u0644 \u0627\u0632 \u0628\u0627\u0632 \u06a9\u0631\u062f\u0646\u060c gBlock \u0644\u06cc\u0648\u0641\u06cc\u0644\u06cc\u0632\u0647 \u0634\u062f\u0647 (\u06f2\u06f5\u06f0 \u0646\u0627\u0646\u0648\u06af\u0631\u0645) \u0631\u0627 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0622\u0646 \u0631\u0627 \u062f\u0631 \u0647\u0645\u0627\u0646 \u0644\u0648\u0644\u0647 \u0627\u0635\u0644\u06cc \u0628\u0627 \u200e500 \u00b5L\u200e \u0628\u0627\u0641\u0631 \u0631\u0642\u06cc\u0642\u200c\u06a9\u0646\u0646\u062f\u0647 TE \u0647\u06cc\u062f\u0631\u0627\u062a\u0647 \u06a9\u0646\u06cc\u062f. \u06a9\u0645\u06cc \u0628\u0627 \u0648\u0631\u062a\u06a9\u0633 \u0645\u062e\u0644\u0648\u0637 \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0647 \u0645\u062f\u062a \u06f2\u06f0 \u062f\u0642\u06cc\u0642\u0647 \u062f\u0631 \u062f\u0645\u0627\u06cc \u200e50\u200e \u062f\u0631\u062c\u0647 \u0633\u0644\u0633\u06cc\u0648\u0633 \u0642\u0631\u0627\u0631 \u062f\u0647\u06cc\u062f. \u0633\u067e\u0633 \u062f\u0648\u0628\u0627\u0631\u0647 \u06a9\u0645\u06cc \u0648\u0631\u062a\u06a9\u0633 \u06a9\u0631\u062f\u0647 \u0648 \u06cc\u06a9 \u0628\u0627\u0631 \u062f\u06cc\u06af\u0631 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u062a\u0645\u0627\u0645 \u0645\u0627\u06cc\u0639 \u0628\u0647 \u0627\u0646\u062a\u0647\u0627\u06cc \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">2E7<\/span><\/p>\n<\/td>\n<td style=\"width: 50%;\" width=\"312\"><span style=\"font-size: 14pt;\">\u06f1\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0627\u0632 \u0645\u062d\u0644\u0648\u0644 \u0630\u062e\u06cc\u0631\u0647 2E9 \u0631\u0627 \u0628\u0627 \u06f9\u06f9\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0628\u0627\u0641\u0631 TE \u062f\u0631 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u062f\u06cc\u062f \u0645\u062e\u0644\u0648\u0637 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0644\u0648\u0644\u0647 \u0631\u0627 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u062a\u0645\u0627\u0645 \u0645\u0627\u06cc\u0639 \u0628\u0647 \u0627\u0646\u062a\u0647\u0627\u06cc \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">2E5<\/span><\/p>\n<\/td>\n<td style=\"width: 50%;\" width=\"312\"><span style=\"font-size: 14pt;\">\u06f1\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0627\u0632 \u0631\u0642\u062a \u00a02E7 \u0631\u0627 \u0628\u0627 \u06f9\u06f9\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0628\u0627\u0641\u0631 TE \u062f\u0631 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u062f\u06cc\u062f \u0645\u062e\u0644\u0648\u0637 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0644\u0648\u0644\u0647 \u0631\u0627 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u062a\u0645\u0627\u0645 \u0645\u062d\u0644\u0648\u0644 \u0628\u0647 \u062a\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">5E3<\/span><\/p>\n<\/td>\n<td style=\"width: 50%;\" width=\"312\"><span style=\"font-size: 14pt;\">\u06f1\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0627\u0632 \u0631\u0642\u062a 2E5 \u0631\u0627 \u0628\u0627 \u06f3\u06f9\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0628\u0627\u0641\u0631 TE \u062f\u0631 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u062f\u06cc\u062f \u0645\u062e\u0644\u0648\u0637 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0644\u0648\u0644\u0647 \u0631\u0627 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u062a\u0645\u0627\u0645 \u0645\u062d\u0644\u0648\u0644 \u0628\u0647 \u062a\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">5E2<\/span><\/p>\n<\/td>\n<td style=\"width: 50%;\" width=\"312\"><span style=\"font-size: 14pt;\">\u06f5\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0627\u0632 \u0631\u0642\u062a 5E3 \u0631\u0627 \u0628\u0627 \u06f4\u06f5\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0628\u0627\u0641\u0631 TE \u062f\u0631 \u06cc\u06a9 \u0644\u0648\u0644\u0647 \u062c\u062f\u06cc\u062f \u0645\u062e\u0644\u0648\u0637 \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u0644\u0648\u0644\u0647 \u0631\u0627 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u062a\u0645\u0627\u0645 \u0645\u062d\u0644\u0648\u0644 \u0628\u0647 \u062a\u0647 \u0644\u0648\u0644\u0647 \u062c\u0645\u0639 \u0634\u0648\u062f.<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\"><strong>\u062a\u0648\u062c\u0647<\/strong><strong>:<\/strong> \u0627\u06cc\u0646 \u0645\u0642\u062f\u0627\u0631 \u0628\u0647 \u0635\u0648\u0631\u062a \u0646\u0638\u0631\u06cc \u0645\u062d\u0627\u0633\u0628\u0647 \u0634\u062f\u0647 \u0648 \u0628\u0633\u062a\u06af\u06cc \u0628\u0647 \u063a\u0644\u0638\u062a DNA\u060c \u0639\u062f\u062f \u0622\u0648\u0648\u06af\u0627\u062f\u0631\u0648\u060c \u0637\u0648\u0644 \u0642\u0627\u0644\u0628 \u0648 \u0648\u0632\u0646 \u0645\u062a\u0648\u0633\u0637 \u0647\u0631 \u062c\u0641\u062a \u0628\u0627\u0632 \u062f\u0627\u0631\u062f. \u0628\u0631\u0627\u06cc \u0628\u0647\u200c\u062f\u0633\u062a \u0622\u0648\u0631\u062f\u0646 \u0627\u06cc\u0646 \u0645\u0642\u062f\u0627\u0631\u060c \u0645\u0627\u0634\u06cc\u0646\u200c\u062d\u0633\u0627\u0628\u200c\u0647\u0627\u06cc \u0622\u0646\u0644\u0627\u06cc\u0646 \u0631\u0627\u06cc\u06af\u0627\u0646\u06cc \u0647\u0645 \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>1.9.3. \u0645\u062d\u0627\u0633\u0628\u0647 \u062a\u0639\u062f\u0627\u062f \u062a\u0642\u0631\u06cc\u0628\u06cc \u0646\u0633\u062e\u0647\u200c\u0647\u0627\u06cc DNA<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u0632 \u0645\u0642\u062f\u0627\u0631(250 \u0646\u0627\u0646\u0648\u06af\u0631\u0645) \u00a0DNA \u0648 \u0648\u0632\u0646 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u062a\u0648\u0627\u0644\u06cc(\u06f1\u06f5\u06f1\u06f2\u06f2\u06f7.\u06f2) \u0628\u0631\u0627\u06cc \u0645\u062d\u0627\u0633\u0628\u0647 \u062a\u0639\u062f\u0627\u062f \u0646\u0633\u062e\u0647\u200c\u0647\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u06a9\u0646\u06cc\u062f. \u0627\u06cc\u0646 \u062a\u0639\u062f\u0627\u062f \u062a\u0642\u0631\u06cc\u0628\u0627 \u0628\u0631\u0627\u0628\u0631 \u0628\u0627 \u00a0E12 \u0646\u0633\u062e\u0647 \u062e\u0648\u0627\u0647\u062f \u0628\u0648\u062f (\u0628\u0627 \u0641\u0631\u0636 \u0627\u06cc\u0646\u06a9\u0647 \u0647\u0631 \u062c\u0641\u062a \u0628\u0627\u0632 DNA \u0648\u0632\u0646\u06cc \u0628\u0631\u0627\u0628\u0631 \u06f6\u06f5\u06f0 \u062f\u0627\u0644\u062a\u0648\u0646 \u062f\u0627\u0631\u062f). \u0686\u0648\u0646 \u0647\u062f\u0641 \u0644\u06cc\u0648\u0641\u06cc\u0644\u06cc\u0632\u0647 (\u067e\u0648\u062f\u0631 DNA) \u062f\u0631 \u06f5\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 TE \u062d\u0644 \u0634\u062f\u0647 \u0627\u0633\u062a\u060c \u062a\u0639\u062f\u0627\u062f \u062a\u0642\u0631\u06cc\u0628\u06cc \u0646\u0633\u062e\u0647\u200c\u0647\u0627 \u0628\u0631\u0627\u0628\u0631 \u0628\u0627 \u00a02E9 \u0646\u0633\u062e\u0647 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0645\u06cc\u200c\u0628\u0627\u0634\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>2.9.3. \u0631\u0648\u0634 \u0627\u062e\u062a\u06cc\u0627\u0631\u06cc \u0628\u0631\u0627\u06cc \u0627\u0646\u062f\u0627\u0632\u0647\u200c\u06af\u06cc\u0631\u06cc \u062f\u0642\u06cc\u0642<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0645\u06cc\u200c\u062a\u0648\u0627\u0646\u06cc\u062f \u063a\u0644\u0638\u062a DNA (\u0645\u0627\u062f\u0647 \u0644\u06cc\u0648\u0641\u06cc\u0644\u06cc\u0632\u0647 \u0634\u062f\u0647 \u062d\u0644 \u0634\u062f\u0647 \u062f\u0631 \u06f5\u06f0\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 TE ) \u0631\u0627 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u06a9\u06cc\u062a Qubit \u0628\u0627 \u062d\u0633\u0627\u0633\u06cc\u062a \u0628\u0627\u0644\u0627 \u06cc\u0627 Nanodrop \u0627\u0646\u062f\u0627\u0632\u0647\u200c\u06af\u06cc\u0631\u06cc \u06a9\u0646\u06cc\u062f. \u0633\u067e\u0633 \u063a\u0644\u0638\u062a \u0628\u0647 \u062f\u0633\u062a \u0622\u0645\u062f\u0647 (ng\/\u00b5L) \u0631\u0627 \u062f\u0631 \u06cc\u06a9 \u0645\u0627\u0634\u06cc\u0646\u200c\u062d\u0633\u0627\u0628 \u062a\u0639\u062f\u0627\u062f \u0646\u0633\u062e\u0647\u200c\u0647\u0627 \u0648\u0627\u0631\u062f \u06a9\u0631\u062f\u0647 \u0648 \u062a\u0639\u062f\u0627\u062f \u062f\u0642\u06cc\u0642 \u0646\u0633\u062e\u0647\u200c\u0647\u0627 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 (copies\/\u00b5L) \u0631\u0627 \u0645\u062d\u0627\u0633\u0628\u0647 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<\/ul>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0627 \u0627\u06cc\u0646 \u0645\u0642\u062f\u0627\u0631 \u0645\u06cc\u200c\u062a\u0648\u0627\u0646 \u0645\u062d\u0644\u0648\u0644 \u0631\u0627 \u0631\u0642\u06cc\u0642 \u06a9\u0631\u062f \u062a\u0627 \u0628\u0647 \u063a\u0644\u0638\u062a \u00a05E2 copies\/\u00b5L \u0628\u0631\u0633\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">10.3. <\/span><span style=\"font-size: 18pt;\">\u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0648 \u0627\u062c\u0631\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0631\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634 \u0647\u062f\u0641 \u00a0C. cayetanensis \u0648 \u0647\u0645\u0686\u0646\u06cc\u0646 \u0647\u062f\u0641 IAC\u060c \u0645\u06cc\u06a9\u0633 \u067e\u0631\u0627\u06cc\u0645\u0631\/\u067e\u0631\u0648\u0628 \u0628\u0627\u06cc\u062f \u062a\u0647\u06cc\u0647 \u0634\u0648\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0642\u0628\u0644 \u0627\u0632 \u062a\u0631\u06a9\u06cc\u0628 \u0645\u062e\u0644\u0648\u0637\u200c\u0647\u0627\u060c \u0647\u0645\u0647 \u0648\u0627\u06a9\u0646\u0634\u200c\u06af\u0631\u0647\u0627 \u0631\u0627 \u0628\u0647\u200c\u0637\u0648\u0631 \u06a9\u0648\u062a\u0627\u0647 \u0645\u062e\u0644\u0648\u0637 (vortex) \u06a9\u0631\u062f\u0647 \u0648 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u062d\u062a\u0648\u06cc\u0627\u062a \u062f\u0648\u0628\u0627\u0631\u0647 \u0645\u0639\u0644\u0642 \u0634\u062f\u0647 \u0648 \u062a\u0647\u200c\u0646\u0634\u06cc\u0646 \u0646\u0634\u0648\u0646\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4 style=\"text-align: justify;\">1.10.3. \u0645\u06cc\u06a9\u0633\u200c\u0647\u0627\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631\/\u067e\u0631\u0648\u0628 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc : Cyclospora cayetanensis<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06cc\u0646 \u0645\u06cc\u06a9\u0633\u200c\u0647\u0627 \u0628\u0627\u06cc\u062f \u062f\u0631 \u062f\u0645\u0627\u06cc \u06f2\u06f0<strong>&#8211;<\/strong> \u062f\u0631\u062c\u0647 \u0633\u0627\u0646\u062a\u06cc\u200c\u06af\u0631\u0627\u062f \u0648 \u062f\u0648\u0631 \u0627\u0632 \u0646\u0648\u0631 \u0646\u06af\u0647\u062f\u0627\u0631\u06cc \u0634\u0648\u0646\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: center;\"><span style=\"font-size: 18pt;\">\u062c\u062f\u0648\u0644 8. \u0645\u062d\u0644\u0648\u0644\u00a0 Mit1C Pr\/Pro20X \u0634\u0627\u0645\u0644 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0628\u0627 \u063a\u0644\u0638\u062a \u0647\u0631 \u06a9\u062f\u0627\u0645\u00a0 \u06f1\u06f2\u00b5M \u0648 \u067e\u0631\u0648\u0628 \u0628\u0627 \u063a\u0644\u0638\u062a \u00b5M\u06f6<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 49.875%;\" width=\"287\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u062d\u062c\u0645<\/strong><\/span><\/p>\n<\/td>\n<td style=\"width: 50%;\" width=\"288\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"287\"><span style=\"font-size: 14pt;\">\u06f1\u06f2.\u06f0 \u00b5L \u067e\u0631\u0627\u06cc\u0645\u0631 Mit1C-f \u00a0\u0628\u0627 \u063a\u0644\u0638\u062a \u06f5\u06f0\u06f0 \u00b5M<\/span><\/td>\n<td style=\"width: 50%; text-align: center;\" width=\"288\"><span style=\"font-size: 14pt;\">\u06f0.\u06f6 \u00b5M \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634 \u0646\u0647\u0627\u06cc\u06cc (\u0628\u0631\u0627\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631\u200c\u0647\u0627)<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"287\"><span style=\"font-size: 14pt;\">\u06f1\u06f2.\u06f0 \u00b5L \u067e\u0631\u0627\u06cc\u0645\u0631 Mit1C-r \u00a0\u0628\u0627 \u063a\u0644\u0638\u062a \u06f5\u06f0\u06f0 \u00b5M<\/span><\/td>\n<td style=\"width: 50%; text-align: center;\" width=\"288\"><span style=\"font-size: 14pt;\">\u06f0.\u06f6 \u00b5M \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634 \u0646\u0647\u0627\u06cc\u06cc (\u0628\u0631\u0627\u06cc \u067e\u0631\u0627\u06cc\u0645\u0631\u200c\u0647\u0627)<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"287\"><span style=\"font-size: 14pt;\">\u06f3\u06f0.\u06f0 \u00b5L \u067e\u0631\u0648\u0628 \u00a0Mit1P-FAM \u0628\u0627 \u063a\u0644\u0638\u062a \u06f1\u06f0\u06f0 \u00b5M<\/span><\/td>\n<td style=\"width: 50%; text-align: center;\" width=\"288\"><span style=\"font-size: 14pt;\">\u06f0.\u06f3 \u00b5M (\u0628\u0631\u0627\u06cc \u067e\u0631\u0648\u0628) \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634 \u0646\u0647\u0627\u06cc\u06cc<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"287\"><span style=\"font-size: 14pt;\">\u06f4\u06f4\u06f6 \u00b5L \u0628\u0627\u0641\u0631 TE<\/span><\/td>\n<td style=\"width: 50%;\" width=\"288\"><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"287\"><span style=\"font-size: 14pt;\">\u062d\u062c\u0645 \u0646\u0647\u0627\u06cc\u06cc \u06f5\u06f0\u06f0 \u00b5L<\/span><\/td>\n<td style=\"width: 50%;\" width=\"288\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 9. \u0645\u062d\u0644\u0648\u0644 X synIAC Pr\/Pro 20\u0634\u0627\u0645\u0644 \u067e\u0631\u0627\u06cc\u0645\u0631\u0647\u0627 \u0628\u0627 \u063a\u0644\u0638\u062a \u0647\u0631 \u06a9\u062f\u0627\u0645 \u06f5\u00b5M\u060c \u067e\u0631\u0648\u0628 \u0628\u0627 \u063a\u0644\u0638\u062a \u06f5 \u00b5M\u060c \u062d\u0627\u0648\u06cc 10<sup>5<\/sup>\u00d7 \u06f2\u0646\u0633\u062e\u0647 \u0627\u0632 \u0647\u062f\u0641\u00a0 synIAC<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 35.125%; text-align: right;\" width=\"202\"><span style=\"font-size: 14pt;\"><strong>\u062d\u062c\u0645<\/strong><\/span><\/td>\n<td style=\"width: 34.875%; text-align: right;\" width=\"201\"><span style=\"font-size: 14pt;\"><strong>\u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc<\/strong><\/span><\/td>\n<td style=\"width: 29.875%; text-align: right;\" width=\"172\"><span style=\"font-size: 14pt;\"><strong>\u062d\u062c\u0645 \u0645\u062d\u062a\u0648\u06cc \u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc \u0648\u0627\u06a9\u0646\u0634<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 35.125%; text-align: center;\" width=\"202\"><span style=\"font-size: 14pt;\">\u06f5.\u06f0 \u00b5L \u067e\u0631\u0627\u06cc\u0645\u0631 dd-IAC-f \u0628\u0627 \u063a\u0644\u0638\u062a \u06f5\u06f0\u06f0 \u00b5M<\/span><\/td>\n<td style=\"width: 34.875%; text-align: center;\" width=\"201\"><span style=\"font-size: 14pt;\">\u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634: \u06f0.\u06f2\u06f5 \u00b5M<\/span><\/td>\n<td style=\"width: 29.875%;\" width=\"172\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u06f5\u06f0\u06f0 \u00b5L<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 35.125%; text-align: center;\" width=\"202\"><span style=\"font-size: 14pt;\">\u06f5.\u06f0 \u00b5L \u067e\u0631\u0627\u06cc\u0645\u0631 dd-IAC-r \u0628\u0627 \u063a\u0644\u0638\u062a \u06f5\u06f0\u06f0 \u00b5M<\/span><\/td>\n<td style=\"width: 34.875%; text-align: center;\" width=\"201\"><span style=\"font-size: 14pt;\">\u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634: \u06f0.\u06f2\u06f5 \u00b5M<\/span><\/td>\n<td style=\"width: 29.875%;\" width=\"172\"><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 35.125%; text-align: center;\" width=\"202\"><span style=\"font-size: 14pt;\">\u06f2\u06f5.\u06f0 \u00b5L \u067e\u0631\u0648\u0628 dd-IAC-Cy5 \u0628\u0627 \u063a\u0644\u0638\u062a \u06f1\u06f0\u06f0 \u00b5M<\/span><\/td>\n<td style=\"width: 34.875%; text-align: center;\" width=\"201\"><span style=\"font-size: 14pt;\">\u063a\u0644\u0638\u062a \u0646\u0647\u0627\u06cc\u06cc \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634: \u06f0.\u06f2\u06f5 \u00b5M<\/span><\/td>\n<td style=\"width: 29.875%;\" width=\"172\"><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 35.125%; text-align: center;\" width=\"202\"><span style=\"font-size: 14pt;\">\u06f1\u06f0 \u00b5L \u0647\u062f\u0641 synIAC HMultra130 \u0628\u0627 \u063a\u0644\u0638\u062a 1\u00d710\u2077 \u0646\u0633\u062e\u0647\u00b5L\/<\/span><\/td>\n<td style=\"width: 34.875%; text-align: center;\" width=\"201\"><span style=\"font-size: 14pt;\">\u062a\u0639\u062f\u0627\u062f \u0646\u0633\u062e\u0647 \u0646\u0647\u0627\u06cc\u06cc \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634: 1\u00d710\u2074 \u0646\u0633\u062e\u0647 \u062f\u0631 \u0647\u0631 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 (1E4 copies\/\u00b5L)<\/span><\/td>\n<td style=\"width: 29.875%;\" width=\"172\"><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 35.125%; text-align: center;\" width=\"202\"><span style=\"font-size: 14pt;\">\u0628\u0627\u0641\u0631 TE 455 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631<\/span><\/td>\n<td style=\"width: 34.875%; text-align: center;\" width=\"201\"><span style=\"font-size: 14pt;\"><strong>\u00a0<\/strong><\/span><\/td>\n<td style=\"width: 29.875%;\" width=\"172\"><span style=\"font-size: 14pt;\"><strong>\u00a0<\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4 style=\"text-align: justify;\">\u00a02.10.3. \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 Real Time PCR \u0628\u0631\u0627\u06cc \u062d\u062c\u0645 \u06f2\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0645\u0627\u0645 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627 \u0648 \u06a9\u0646\u062a\u0631\u0644\u200c\u0647\u0627 \u0647\u0645\u06cc\u0634\u0647 \u0628\u0647 \u0635\u0648\u0631\u062a \u0633\u0647 \u062a\u0627\u06cc\u06cc (triplicate) \u0627\u062c\u0631\u0627 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0642\u0628\u0644 \u0627\u0632 \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634\u060c \u0647\u0645\u0647 \u0648\u0627\u06a9\u0646\u0634\u200c\u06af\u0631\u0647\u0627 \u0631\u0627 \u0628\u0647\u200c\u0637\u0648\u0631 \u06a9\u0648\u062a\u0627\u0647 \u0645\u062e\u0644\u0648\u0637 \u0648 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0645\u062d\u062a\u0648\u06cc\u0627\u062a \u062f\u0648\u0628\u0627\u0631\u0647 \u0645\u0639\u0644\u0642 \u0634\u0648\u0646\u062f \u0648 \u062a\u0647\u200c\u0646\u0634\u06cc\u0646 \u0646\u06af\u0631\u062f\u0646\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0641\u0631\u0645\u0648\u0644 \u0645\u0633\u062a\u0631 \u0645\u06cc\u06a9\u0633 \u0632\u06cc\u0631 \u0628\u0631\u0627\u06cc \u0627\u062c\u0631\u0627\u06cc \u06cc\u06a9 \u062a\u06a9\u0631\u0627\u0631 (replicate) \u0627\u0632 \u06cc\u06a9 \u0646\u0645\u0648\u0646\u0647 \u06a9\u0627\u0641\u06cc \u0627\u0633\u062a.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0628\u0631\u0627\u06cc \u0647\u0631 \u0627\u062c\u0631\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634 qPCR\u060c \u062d\u062c\u0645 \u06a9\u0627\u0641\u06cc \u0627\u0632 \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u0631\u0627 \u0622\u0645\u0627\u062f\u0647 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u06a9\u0646\u062a\u0631\u0644 \u0628\u062f\u0648\u0646 \u0642\u0627\u0644\u0628 (NTC)\u060c \u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a \u0648 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u060c \u0647\u0645\u0647 \u0628\u0647 \u0634\u06a9\u0644 \u0633\u0647 \u062a\u0627\u06cc\u06cc \u0627\u062c\u0631\u0627 \u0634\u0648\u0646\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0639\u062f\u0627\u062f \u06a9\u0644 \u062a\u06a9\u0631\u0627\u0631\u0647\u0627 (N) \u0631\u0627 \u0628\u0631\u0627\u06cc \u06cc\u06a9 \u0622\u0632\u0645\u0627\u06cc\u0634 \u0645\u062d\u0627\u0633\u0628\u0647 \u06a9\u0646\u06cc\u062f \u0648 \u062d\u062c\u0645 \u0645\u0633\u062a\u0631 \u0645\u06cc\u06a9\u0633 \u0631\u0627 \u0628\u06cc\u0646 N+1 <strong>\u00a0\u062a\u0627<\/strong> N+3 \u0622\u0645\u0627\u062f\u0647 \u06a9\u0646\u06cc\u062f \u062a\u0627 \u0628\u0631\u0627\u06cc \u0647\u0645\u0647 \u062a\u06a9\u0631\u0627\u0631\u0647\u0627 \u062d\u062c\u0645 \u06a9\u0627\u0641\u06cc \u0648\u062c\u0648\u062f \u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u062f<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 10. \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u0628\u0631\u0627\u06cc \u062d\u062c\u0645 \u06f2\u06f0 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 69.125%;\" width=\"378\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u0627\u062c\u0632\u0627\u06cc \u0645\u062e\u0644\u0648\u0637 \u0627\u0635\u0644\u06cc<\/strong><strong> (Master Mix Components)<\/strong><\/span><\/p>\n<\/td>\n<td style=\"width: 30.75%; text-align: right;\" width=\"168\"><span style=\"font-size: 14pt;\"><strong>\u062d\u062c\u0645<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 69.125%; text-align: center;\" width=\"378\"><span style=\"font-size: 14pt;\">\u00b5L \u0627\u0632 \u0645\u062d\u0644\u0648\u0644 X2 primetime Gene expression master mix \u0628\u0627 \u0631\u0646\u06af \u0645\u0631\u062c\u0639 \u06a9\u0645 (\u0634\u0631\u06a9\u062a IDT)<\/span><\/td>\n<td style=\"width: 30.75%; text-align: center;\" width=\"168\"><span style=\"font-size: 14pt;\">10.0<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 69.125%; text-align: center;\" width=\"378\"><span style=\"font-size: 14pt;\">\u062d\u062c\u0645 \u00b5L \u0627\u0632 \u0645\u062e\u0644\u0648\u0637 \u00a0X\u00a0 20 \u067e\u0631\u0627\u06cc\u0645\u0631\/\u067e\u0631\u0648\u0628 \u00a0Mit1C<\/span><\/td>\n<td style=\"width: 30.75%; text-align: center;\" width=\"168\"><span style=\"font-size: 14pt;\">1.0<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 69.125%; text-align: center;\" width=\"378\"><span style=\"font-size: 14pt;\">\u062d\u062c\u0645 \u00b5L \u0627\u0632 \u0645\u062e\u0644\u0648\u0637 X 20 \u067e\u0631\u0627\u06cc\u0645\u0631\/\u067e\u0631\u0648\u0628 synIAC<\/span><\/td>\n<td style=\"width: 30.75%; text-align: center;\" width=\"168\"><span style=\"font-size: 14pt;\">1.0<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 69.125%; text-align: center;\" width=\"378\"><span style=\"font-size: 14pt;\">\u062d\u062c\u0645 \u0622\u0628 \u062f\u06cc\u0648\u0646\u06cc\u0632\u0647 \u0628\u062f\u0648\u0646 DNase \u0628\u0647 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631<\/span><\/td>\n<td style=\"width: 30.75%; text-align: center;\" width=\"168\"><span style=\"font-size: 14pt;\">6.0<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 69.125%; text-align: center;\" width=\"378\"><span style=\"font-size: 14pt;\">\u062d\u062c\u0645 \u0646\u0645\u0648\u0646\u0647 \u06cc\u0627 \u06a9\u0646\u062a\u0631\u0644 \u0628\u0647 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631<\/span><\/td>\n<td style=\"width: 30.75%; text-align: center;\" width=\"168\"><span style=\"font-size: 14pt;\">2.0<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 69.125%; text-align: center;\" width=\"378\"><span style=\"font-size: 14pt;\">\u062d\u062c\u0645 \u06a9\u0644\u06cc \u0628\u0647 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631<\/span><\/td>\n<td style=\"width: 30.75%; text-align: center;\" width=\"168\"><span style=\"font-size: 14pt;\">20.0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u06f1\u06f8 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0627\u0632 \u0645\u062e\u0644\u0648\u0637 \u0648\u0627\u06a9\u0646\u0634 \u0631\u0627 \u0628\u0647 \u0647\u0631 \u0686\u0627\u0647\u06a9 \u06cc\u0627 \u0644\u0648\u0644\u0647 \u0648\u0627\u06a9\u0646\u0634 \u0645\u0646\u062a\u0642\u0644 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0633\u067e\u0633 \u06f2 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631 \u0627\u0632 \u0646\u0645\u0648\u0646\u0647 \u06cc\u0627 \u06a9\u0646\u062a\u0631\u0644 \u0645\u0646\u0627\u0633\u0628 \u0631\u0627 \u0628\u0647 \u0647\u0631 \u0686\u0627\u0647\u06a9 \u06cc\u0627 \u0644\u0648\u0644\u0647 \u0648\u0627\u06a9\u0646\u0634 \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f (\u062c\u0632\u0626\u06cc\u0627\u062a \u062f\u0631 \u0628\u062e\u0634 3-10-3 \u0627\u0631\u0627\u0626\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a).<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4><a name=\"_Toc208648560\"><\/a>3.10.3.<strong> \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627 \u0648 \u06a9\u0646\u062a\u0631\u0644\u200c\u0647\u0627 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc <\/strong>\u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633<\/h4>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: center;\"><span style=\"font-size: 14pt;\">\u062c\u062f\u0648\u0644 11. \u062d\u062c\u0645 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627 \u0648 \u06a9\u0646\u062a\u0631\u0644\u200c\u0647\u0627<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 49.875%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u0646\u0645\u0648\u0646\u0647 \u0648 \u06a9\u0646\u062a\u0631\u0644 \u0647\u0627<\/strong><\/span><\/p>\n<\/td>\n<td style=\"width: 49.875%;\" width=\"312\">\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt;\"><strong>\u062d\u062c\u0645<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\">NTC<\/span><\/td>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\">\u06f2.\u06f0 \u00b5L \u0622\u0628 \u062f\u06cc\u0648\u0646\u06cc\u0632\u0647 \u0628\u062f\u0648\u0646 \u00a0DNase<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\">\u06a9\u0646\u062a\u0631\u0644 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA<\/span><\/td>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\">2.0 \u00b5L<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\">\u0646\u0645\u0648\u0646\u0647 \u0647\u0627<\/span><\/td>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\">2.0 \u00b5L ( \u0628\u0627 \u063a\u0644\u0638\u062a \u0627\u0635\u0644\u06cc \u0648 \u0631\u0642\u062a \u06cc\u06a9 \u0686\u0647\u0627\u0631\u0645)<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\">\u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a<\/span><\/td>\n<td style=\"width: 49.875%; text-align: center;\" width=\"312\"><span style=\"font-size: 14pt;\">\u06f2 Mit1AA gBlock \u00a0\u00b5L \u0628\u0627 \u063a\u0644\u0638\u062a \u06f5\u00d7\u06f1\u06f0<sup>2<\/sup> \u0646\u0633\u062e\u0647 \u062f\u0631 \u0645\u06cc\u06a9\u0631\u0648\u0644\u06cc\u062a\u0631<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4><strong>\u00a04.10.3. \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627 \u0648 \u06a9\u0646\u062a\u0631\u0644\u200c\u0647\u0627<\/strong><\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0642\u0628\u0644 \u0627\u0632 \u0627\u0636\u0627\u0641\u0647 \u06a9\u0631\u062f\u0646 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627 \u0648 \u06a9\u0646\u062a\u0631\u0644\u200c\u0647\u0627 \u0628\u0647 \u0686\u0627\u0647\u06a9\u200c\u0647\u0627 \u06cc\u0627 \u0644\u0648\u0644\u0647\u200c\u0647\u0627\u06cc \u0648\u0627\u06a9\u0646\u0634\u060c \u0647\u0645\u0647 \u0622\u0646\u200c\u0647\u0627 \u0631\u0627 \u0628\u0647\u200c\u0637\u0648\u0631 \u0645\u062e\u062a\u0635\u0631 \u0648\u0631\u062a\u06a9\u0633 \u06a9\u0631\u062f\u0647 \u0648 \u0633\u067e\u0633 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0648\u062a\u0627\u0647 \u0627\u0646\u062c\u0627\u0645 \u062f\u0647\u06cc\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062a\u0645\u0627\u0645 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u0646\u0627\u0634\u0646\u0627\u062e\u062a\u0647 \u0628\u0627\u06cc\u062f \u062f\u0631 \u0647\u0645\u0627\u0646 \u0627\u062c\u0631\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634 \u0627\u0648\u0644\u06cc\u0647\u060c \u0647\u0645 \u0628\u0647\u200c\u0635\u0648\u0631\u062a \u063a\u0644\u0638\u062a \u0627\u0635\u0644\u06cc (1X) \u0648 \u0647\u0645 \u0628\u0647\u200c\u0635\u0648\u0631\u062a \u0631\u0642\u06cc\u0642\u200c\u0634\u062f\u0647 \u00bc \u0622\u0646\u0627\u0644\u06cc\u0632 \u0634\u0648\u0646\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]<span style=\"font-size: 14pt;\">\u062a\u0648\u062c\u0647: \u06a9\u0646\u062a\u0631\u0644\u200c\u0647\u0627 \u062f\u0631 \u0631\u0642\u062a \u00bc \u062a\u0633\u062a \u0646\u0645\u06cc\u200c\u0634\u0648\u0646\u062f.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>5.10.3. \u067e\u0631\u0648\u062a\u06a9\u0644 \u0631\u0642\u06cc\u0642\u200c\u0633\u0627\u0632\u06cc \u00bc\u0646\u0645\u0648\u0646\u0647<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ol>\n<li><span style=\"font-size: 14pt;\">\u0645\u0642\u062f\u0627\u0631 5\u00b5L \u0627\u0632 \u0646\u0645\u0648\u0646\u0647 \u0631\u0627 \u0628\u0647 \u06cc\u06a9 \u0645\u06cc\u06a9\u0631\u0648\u062a\u06cc\u0648\u0628 \u062a\u0645\u06cc\u0632 \u0645\u0646\u062a\u0642\u0644 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<li><span style=\"font-size: 14pt;\">\u0628\u0647 \u0622\u0646 5\u00b5L \u0628\u0627\u0641\u0631 TE \u0627\u0636\u0627\u0641\u0647 \u06a9\u0646\u06cc\u062f.<\/span><\/li>\n<\/ol>\n<p><span style=\"font-size: 14pt;\">\u0628\u0647\u200c\u062e\u0648\u0628\u06cc \u0645\u062e\u0644\u0648\u0637 \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0647\u200c\u0637\u0648\u0631 \u06a9\u0648\u062a\u0627\u0647 \u0633\u0627\u0646\u062a\u0631\u06cc\u0641\u06cc\u0648\u0698 \u06a9\u0646\u06cc\u062f.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>6.10.3. \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u067e\u0644\u06cc\u062a \u06cc\u0627 \u0644\u0648\u0644\u0647\u200c\u0647\u0627 \u0628\u0631\u0627\u06cc \u0627\u062c\u0631\u0627<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u067e\u0644\u06cc\u062a \u06cc\u0627 \u0646\u0648\u0627\u0631 \u0644\u0648\u0644\u0647\u200c\u0647\u0627 \u0631\u0627 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 \u062f\u0633\u062a\u06af\u0627\u0647 Fast Real-Time PCR ABI 7500 \u0637\u0628\u0642 \u0627\u0644\u06af\u0648\u06cc \u067e\u0631\u0648\u062a\u06a9\u0644 \u0627\u0632 \u067e\u06cc\u0634 \u062a\u0639\u06cc\u06cc\u0646\u200c\u0634\u062f\u0647 \u0648 \u0631\u0648\u0634 \u0627\u062c\u0631\u0627\u06cc \u062a\u0648\u0636\u06cc\u062d \u062f\u0627\u062f\u0647 \u200c\u0634\u062f\u0647 \u062f\u0631 \u067e\u06cc\u0648\u0633\u062a \u060c \u00a0\u0628\u0647 \u062c\u0647\u062a \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0631\u0627\u0647\u200c\u0627\u0646\u062f\u0627\u0632\u06cc \u0648 \u0627\u062c\u0631\u0627 \u06a9\u0646\u06cc\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3><span style=\"font-size: 18pt;\">\u062e\u0637 Threshold<\/span><\/h3>\n[\/vc_column_text][vc_message message_box_color=&#8221;pink&#8221; icon_fontawesome=&#8221;fa fa-solid fa-hand-point-right&#8221; css=&#8221;&#8221;]<span style=\"font-size: 18pt;\"><strong>\u0628\u06cc\u0634\u062a\u0631 \u0628\u062f\u0627\u0646\u06cc\u062f<\/strong><\/span><\/p>\n<p><span style=\"font-size: 14pt;\">\u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u200c\u0647\u0627\u06cc \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0645\u062b\u0644 Real-Time PCR\u060c \u0645\u0646\u0638\u0648\u0631 \u0627\u0632\u00a0\u00a0<\/span><span style=\"font-size: 18.6667px;\">Threshold<\/span><span style=\"font-size: 14pt;\">:<\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt;\">\u06cc\u06a9 \u062e\u0637 \u0645\u0628\u0646\u0627 \u06cc\u0627 \u0633\u0637\u062d \u0645\u0634\u062e\u0635 \u0627\u0632 \u0633\u06cc\u06af\u0646\u0627\u0644 \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0627\u0633\u062a.<\/span><\/li>\n<li><span style=\"font-size: 14pt;\">\u0648\u0642\u062a\u06cc \u0634\u062f\u062a \u0641\u0644\u0648\u0631\u0633\u0627\u0646\u0633 \u0646\u0645\u0648\u0646\u0647 \u0627\u0632 \u0627\u06cc\u0646 \u0633\u0637\u062d \u0628\u0627\u0644\u0627\u062a\u0631 \u0645\u06cc\u200c\u0631\u0648\u062f\u060c \u06cc\u0639\u0646\u06cc \u0648\u0627\u06a9\u0646\u0634 PCR \u0648\u0627\u0642\u0639\u0627\u064b \u0634\u0631\u0648\u0639 \u0628\u0647 \u062a\u06a9\u062b\u06cc\u0631 \u06a9\u0631\u062f\u0647 \u0648 \u0646\u062a\u06cc\u062c\u0647 \u0645\u062b\u0628\u062a \u0645\u062d\u0633\u0648\u0628 \u0645\u06cc\u200c\u0634\u0648\u062f.<\/span><\/li>\n<li><span style=\"font-size: 14pt;\">\u0686\u0631\u062e\u0647\u200c\u0627\u06cc \u06a9\u0647 \u062f\u0631 \u0622\u0646\u060c \u0633\u06cc\u06af\u0646\u0627\u0644 \u0627\u0632 \u0622\u0633\u062a\u0627\u0646\u0647 \u0639\u0628\u0648\u0631 \u0645\u06cc\u200c\u06a9\u0646\u062f \u0631\u0627 Ct (Cycle threshold) \u00a0\u06cc\u0627 &#8220;\u0686\u0631\u062e\u0647 \u0622\u0633\u062a\u0627\u0646\u0647&#8221; \u0645\u06cc\u200c\u06af\u0648\u06cc\u0646\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_message][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h3 style=\"text-align: justify;\"><span style=\"font-size: 18pt;\">11.3. \u062a\u0641\u0633\u06cc\u0631 \u0646\u062a\u0627\u06cc\u062c \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/span><\/h3>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<h4>1.11.3. \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u0645\u062b\u0628\u062a<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u062a\u0646\u0647\u0627 \u0632\u0645\u0627\u0646\u06cc \u0628\u0631\u0627\u06cc \u0648\u062c\u0648\u062f \u00a0\u202fC. cayetanensis\u202f \u0645\u062b\u0628\u062a \u062f\u0631 \u0646\u0638\u0631 \u06af\u0631\u0641\u062a\u0647 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f \u06a9\u0647:<\/span><\/p>\n<ol>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u062a\u0633\u062a \u0627\u0648\u0644\u06cc\u0647 \u06cc\u0627 \u062f\u0631 \u062a\u0633\u062a \u062a\u06a9\u0631\u0627\u0631\u06cc\u060c \u062f\u0633\u062a\u200c\u06a9\u0645 \u06cc\u06a9 \u062a\u06a9\u0631\u0627\u0631 \u0646\u0645\u0648\u0646\u0647 \u06cc\u06a9 \u0633\u06cc\u06af\u0646\u0627\u0644 \u062a\u0642\u0648\u06cc\u062a\u06cc \u0635\u0627\u0641 \u0648 \u0646\u0645\u0627\u06cc\u06cc\/\u0633\u06cc\u06af\u0645\u0648\u0626\u06cc\u062f\u06cc \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0645\u0642\u062f\u0627\u0631 ct\u226438 \u0628\u0631\u0627\u06cc \u0647\u062f\u0641 \u0645\u06cc\u062a\u0648\u06a9\u0646\u062f\u0631\u06cc\u0627\u06cc\u06cc C. cayetanensis (Mit1C) \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u062b\u0628\u062a \u0634\u0648\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0648\u0627\u06a9\u0646\u0634 \u0647\u062f\u0641 IAC \u0645\u06cc\u200c\u062a\u0648\u0627\u0646\u062f \u0645\u0646\u0641\u06cc \u06cc\u0627 \u0645\u062b\u0628\u062a \u0628\u0627\u0634\u062f.<\/span><\/li>\n<\/ol>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4 style=\"text-align: justify;\"><strong>2.11.3. \u0646\u0645\u0648\u0646\u0647 \u0647\u0627\u06cc \u0646\u06cc\u0627\u0632\u0645\u0646\u062f \u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0634\u062a\u0631 \u062f\u0631 <\/strong>\u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc\u06cc \u0646\u06cc\u0627\u0632 \u0628\u0647 \u0628\u0631\u0631\u0633\u06cc \u0628\u06cc\u0634\u062a\u0631 \u062f\u0627\u0631\u0646\u062f \u06a9\u0647 \u06cc\u06a9 \u0646\u0645\u0648\u0646\u0647 \u062f\u0631 \u06cc\u06a9 \u06cc\u0627 \u0686\u0646\u062f \u062a\u06a9\u0631\u0627\u0631\u060c \u0628\u0631\u0627\u06cc \u0647\u062f\u0641 \u00a0C. cayetanensis Mit1C \u0633\u06cc\u06af\u0646\u0627\u0644 \u062a\u0642\u0648\u06cc\u062a\u06cc \u0646\u0645\u0627\u06cc\u06cc\/\u0633\u06cc\u06af\u0645\u0648\u0626\u06cc\u062f\u06cc \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u062f\u060c \u0627\u0645\u0627 \u0645\u0642\u062f\u0627\u0631 Ct &gt; 38.0 \u00a0\u0628\u0627\u0634\u062f \u0648 \u0648\u0627\u06a9\u0646\u0634 \u0647\u062f\u0641 \u00a0IAC \u0645\u062b\u0628\u062a \u06cc\u0627 \u0645\u0646\u0641\u06cc \u0634\u0648\u062f. \u062f\u0631 \u0627\u06cc\u0646 \u062d\u0627\u0644\u062a:<\/span><\/p>\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647\u200c\u0647\u0627\u06cc \u0631\u0633\u0645\u06cc \u0646\u0638\u0627\u0631\u062a\u06cc: \u0628\u0627\u06cc\u062f \u0628\u0627 \u00a0HFP SME \u0645\u0634\u0648\u0631\u062a \u0634\u0648\u062f. \u0628\u0633\u062a\u0647 \u0628\u0647 \u0646\u0638\u0631 SME\u060c \u0645\u0645\u06a9\u0646 \u0627\u0633\u062a \u0644\u0627\u0632\u0645 \u0628\u0627\u0634\u062f \u0646\u0645\u0648\u0646\u0647 \u06cc\u06a9\u200c\u0628\u0627\u0631 \u062f\u06cc\u06af\u0631 \u062f\u0631 \u0633\u0647\u200c\u062a\u06a9\u0631\u0627\u0631 (triplicate) \u0647\u0645 \u062f\u0631 \u063a\u0644\u0638\u062a \u00a01X \u0648 \u0647\u0645 \u062f\u0631 \u00bc \u00a0\u0631\u0642\u062a \u0645\u0648\u0631\u062f \u0622\u0632\u0645\u0627\u06cc\u0634 \u0642\u0631\u0627\u0631 \u06af\u06cc\u0631\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4>3.11.3.\u00a0 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u0645\u0646\u0641\u06cc<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc\u06cc \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0645\u0646\u0641\u06cc \u062f\u0631 \u0646\u0638\u0631 \u06af\u0631\u0641\u062a\u0647 \u0645\u06cc\u200c\u0634\u0648\u0646\u062f \u06a9\u0647:<\/span><\/p>\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0648\u0627\u06a9\u0646\u0634 \u0647\u062f\u0641 Mit1C\u202fC. cayetanensis \u062f\u0631 \u0647\u0645\u0647 \u062a\u06a9\u0631\u0627\u0631\u0647\u0627 \u0645\u0642\u062f\u0627\u0631 Ct \u0645\u0634\u062e\u0635\u06cc \u0646\u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0647\u06cc\u0686 \u062a\u06a9\u0631\u0627\u0631\u06cc Ct \u06a9\u0645\u062a\u0631 \u06cc\u0627 \u0628\u0631\u0627\u0628\u0631 38 \u0646\u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0648\u0627\u06a9\u0646\u0634 \u0647\u062f\u0641 IAC \u0646\u0645\u0648\u0646\u0647\u060c \u0645\u06cc\u0627\u0646\u06af\u06cc\u0646 Ct\u200c\u0627\u06cc \u062a\u0648\u0644\u06cc\u062f \u06a9\u0646\u062f \u06a9\u0647 \u0628\u06cc\u0634 \u0627\u0632 \u06f3 \u0686\u0631\u062e\u0647 \u0628\u0627\u0644\u0627\u062a\u0631 \u0627\u0632 NTC \u0646\u0628\u0627\u0634\u062f.<\/span><\/li>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u062f\u0631 \u0627\u06cc\u0646 \u062d\u0627\u0644\u062a \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis\u060c \u0646\u0645\u0648\u0646\u0647 \u0645\u0646\u0641\u06cc \u0627\u0633\u062a \u0648 \u0646\u06cc\u0627\u0632\u06cc \u0628\u0647 \u0627\u0642\u062f\u0627\u0645 \u0628\u06cc\u0634\u062a\u0631\u06cc \u0646\u06cc\u0633\u062a.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4 style=\"text-align: justify;\">4.11.3. \u0646\u062a\u0627\u06cc\u062c \u0646\u0627\u0645\u0639\u062a\u0628\u0631 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06af\u0631 \u06cc\u06a9\u06cc \u06cc\u0627 \u0686\u0646\u062f \u062a\u06a9\u0631\u0627\u0631 \u0627\u0632 \u0646\u0645\u0648\u0646\u0647 NTC (\u06a9\u0646\u062a\u0631\u0644 \u0645\u0646\u0641\u06cc) \u06cc\u0627 \u0646\u0645\u0648\u0646\u0647 \u06a9\u0646\u062a\u0631\u0644 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u062f\u0631 \u0648\u0627\u06a9\u0646\u0634\u200c\u0647\u0627\u06cc \u0647\u062f\u0641 Mit1C \u0646\u062a\u06cc\u062c\u0647 \u0645\u062b\u0628\u062a \u0646\u0634\u0627\u0646 \u062f\u0647\u0646\u062f \u0648 \u0627\u0632 \u0622\u0633\u062a\u0627\u0646\u0647 \u0639\u0628\u0648\u0631 \u06a9\u0646\u0646\u062f\u060c \u0627\u062c\u0631\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0646\u0627\u0645\u0639\u062a\u0628\u0631 \u0627\u0633\u062a \u0648 \u0628\u0627\u06cc\u062f \u062a\u06a9\u0631\u0627\u0631 \u0634\u0648\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06af\u0631 \u067e\u0633 \u0627\u0632 \u062a\u06a9\u0631\u0627\u0631 \u0627\u062c\u0631\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634 \u0646\u0627\u0645\u0639\u062a\u0628\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u060c \u0646\u0645\u0648\u0646\u0647 \u06a9\u0646\u062a\u0631\u0644 \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u062f\u0648\u0628\u0627\u0631\u0647 \u0646\u062a\u06cc\u062c\u0647 \u0645\u062b\u0628\u062a \u0628\u062f\u0647\u062f \u0648 \u0646\u0645\u0648\u0646\u0647 NTC \u0645\u0646\u0641\u06cc \u0628\u0627\u0634\u062f\u060c \u0627\u062d\u062a\u0645\u0627\u0644\u0627\u064b \u0641\u0631\u0622\u06cc\u0646\u062f \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u0622\u0644\u0648\u062f\u0647 \u0634\u062f\u0647 \u0627\u0633\u062a. \u062f\u0631 \u0627\u06cc\u0646 \u0635\u0648\u0631\u062a\u060c \u0641\u0631\u0622\u06cc\u0646\u062f \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA \u0628\u0627\u06cc\u062f \u0628\u0631\u0627\u06cc \u06a9\u0644 \u0645\u062c\u0645\u0648\u0639\u0647 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u062f\u0648\u0628\u0627\u0631\u0647 \u0627\u0646\u062c\u0627\u0645 \u0634\u0648\u062f. \u062f\u0631 \u0635\u0648\u0631\u062a \u0627\u0645\u06a9\u0627\u0646 \u0627\u0632 \u0646\u0645\u0648\u0646\u0647\u200c\u0647\u0627\u06cc \u063a\u0630\u0627\u06cc\u06cc \u0634\u0633\u062a\u0647\u200c\u0634\u062f\u0647 \u0627\u0636\u0627\u0641\u06cc \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u06af\u0631\u062f\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<ul>\n<li style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06af\u0631 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u06cc\u06a9\u06cc \u06cc\u0627 \u0686\u0646\u062f \u062a\u06a9\u0631\u0627\u0631 \u0627\u0632 \u0646\u0645\u0648\u0646\u0647 \u06a9\u0646\u062a\u0631\u0644 \u0645\u062b\u0628\u062a \u0628\u0631\u0627\u06cc cayetanensis Mit1C \u0645\u0642\u062f\u0627\u0631 Ct \u0645\u0634\u062e\u0635\u06cc \u0646\u062f\u0627\u0634\u062a\u0647 \u0628\u0627\u0634\u062f\u060c \u0627\u062c\u0631\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634 \u0646\u0627\u0645\u0639\u062a\u0628\u0631 \u0627\u0633\u062a \u0648 \u0628\u0627\u06cc\u062f \u062f\u0648\u0628\u0627\u0631\u0647 \u0627\u0646\u062c\u0627\u0645 \u0634\u0648\u062f.<\/span><\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h4 style=\"text-align: justify;\">5.11.3. \u0646\u062a\u0627\u06cc\u062c \u0646\u0627\u0645\u0634\u062e\u0635 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis<\/h4>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06af\u0631 \u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634 \u0627\u0648\u0644\u06cc\u0647 (\u06cc\u0627 \u067e\u0633 \u0627\u0632 \u062a\u06a9\u0631\u0627\u0631 \u062f\u0631 \u0635\u0648\u0631\u062a \u0646\u06cc\u0627\u0632):<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0646\u0645\u0648\u0646\u0647 \u0647\u06cc\u0686 \u062a\u06a9\u0631\u0627\u0631\u06cc \u0627\u0632 \u0647\u062f\u0641 \u0645\u06cc\u062a\u0648\u06a9\u0646\u062f\u0631\u06cc\u0627\u06cc\u06cc Mit1C \u0627\u0646\u06af\u0644 Ct \u2264 38.0 \u062a\u0648\u0644\u06cc\u062f \u0646\u06a9\u0646\u062f \u0648 \u0633\u06cc\u06af\u0646\u0627\u0644 IAC \u00a0(\u06a9\u0646\u062a\u0631\u0644 \u062f\u0627\u062e\u0644\u06cc) \u0646\u0645\u0648\u0646\u0647 \u06cc\u0627 \u0646\u0627\u0645\u0634\u062e\u0635 \u0628\u0627\u0634\u062f\u060c \u06cc\u0627 \u0645\u06cc\u0627\u0646\u06af\u06cc\u0646 \u00a0Ct \u0622\u0646 \u0628\u06cc\u0634 \u0627\u0632 \u06f3 \u0686\u0631\u062e\u0647 \u0628\u0627\u0644\u0627\u062a\u0631 \u0627\u0632 \u06a9\u0646\u062a\u0631\u0644 \u0645\u0646\u0641\u06cc (NTC) \u0628\u0627\u0634\u062f\u060c \u06cc\u0639\u0646\u06cc \u0646\u062a\u06cc\u062c\u0647 \u0646\u0645\u0648\u0646\u0647 \u0642\u0627\u0628\u0644 \u0627\u0639\u062a\u0645\u0627\u062f \u0646\u06cc\u0633\u062a \u0648 \u0627\u062d\u062a\u0645\u0627\u0644\u0627\u064b \u0645\u0647\u0627\u0631 \u0648\u0627\u06a9\u0646\u0634 PCR \u06cc\u0627 \u0645\u0634\u06a9\u0644 \u062f\u0631 \u0646\u0645\u0648\u0646\u0647 \u062f\u0631 \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 \u0648\u062c\u0648\u062f \u062f\u0627\u0631\u062f.<\/span><\/p>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><a name=\"_Toc208648565\"><\/a><span style=\"font-size: 14pt;\">\u062f\u0631 \u0627\u06cc\u0646 \u062d\u0627\u0644\u062a \u062f\u0631 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647\u200c\u0647\u0627\u06cc \u0631\u0633\u0645\u06cc \u0646\u0638\u0627\u0631\u062a\u06cc: \u0628\u0627\u06cc\u062f \u0628\u0627 <strong>\u00a0<\/strong>HFP SME \u0645\u0634\u0648\u0631\u062a \u0634\u0648\u062f. \u0628\u0633\u062a\u0647 \u0628\u0647 \u0646\u0638\u0631 SME\u060c \u0645\u0645\u06a9\u0646 \u0627\u0633\u062a \u0644\u0627\u0632\u0645 \u0628\u0627\u0634\u062f \u0646\u0645\u0648\u0646\u0647 \u06cc\u06a9\u200c\u0628\u0627\u0631 \u062f\u06cc\u06af\u0631 \u062f\u0631 \u0633\u0647\u200c\u062a\u06a9\u0631\u0627\u0631 (triplicate) \u0647\u0645 \u062f\u0631 \u063a\u0644\u0638\u062a \u00a01X \u0648 \u0647\u0645 \u062f\u0631 \u00bc \u00a0\u0631\u0642\u062a \u0645\u0648\u0631\u062f \u0622\u0632\u0645\u0627\u06cc\u0634 \u0642\u0631\u0627\u0631 \u06af\u06cc\u0631\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<h2><span style=\"font-size: 24pt;\">4.\u00a0 \u0633\u062e\u0646 \u067e\u0627\u06cc\u0627\u0646\u06cc<\/span><\/h2>\n[\/vc_column_text][vc_column_text css=&#8221;&#8221;]\n<p style=\"text-align: justify;\"><span style=\"font-size: 14pt;\">\u0627\u06cc\u0646 \u0631\u0648\u0634 \u0639\u0645\u0644\u06cc\u0627\u062a\u06cc \u0627\u0633\u062a\u0627\u0646\u062f\u0627\u0631\u062f (SOP) \u0627\u0631\u0627\u0626\u0647 \u0634\u062f\u0647 \u0628\u0631\u0627\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u00a0\u062f\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0627\u0632\u0647 \u0628\u0627 \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0627\u0632 PCR \u00a0Real Time\u060c \u06cc\u06a9 \u0631\u0627\u0647\u06a9\u0627\u0631 \u062f\u0642\u06cc\u0642\u060c \u062d\u0633\u0627\u0633 \u0648 \u062a\u06a9\u0631\u0627\u0631\u067e\u0630\u06cc\u0631 \u0631\u0627 \u0628\u0631\u0627\u06cc \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0627\u06cc\u0646 \u0627\u0646\u06af\u0644 \u0641\u0631\u0627\u0647\u0645 \u0645\u06cc\u200c\u0622\u0648\u0631\u062f. \u0631\u0639\u0627\u06cc\u062a \u062f\u0642\u06cc\u0642 \u0645\u0631\u0627\u062d\u0644 \u0646\u0645\u0648\u0646\u0647\u200c\u06af\u06cc\u0631\u06cc\u060c \u0627\u0633\u062a\u062e\u0631\u0627\u062c DNA\u060c \u0622\u0645\u0627\u062f\u0647\u200c\u0633\u0627\u0632\u06cc \u0648\u0627\u06a9\u0646\u0634\u200c\u0647\u0627 \u0648 \u062a\u0646\u0638\u06cc\u0645\u0627\u062a \u062f\u0633\u062a\u06af\u0627\u0647\u060c \u0646\u0647 \u062a\u0646\u0647\u0627 \u062f\u0642\u062a \u0646\u062a\u0627\u06cc\u062c \u0631\u0627 \u062a\u0636\u0645\u06cc\u0646 \u0645\u06cc\u200c\u06a9\u0646\u062f \u0628\u0644\u06a9\u0647 \u0642\u0627\u0628\u0644\u06cc\u062a \u0645\u0642\u0627\u06cc\u0633\u0647 \u0646\u062a\u0627\u06cc\u062c \u0628\u06cc\u0646 \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647\u200c\u0647\u0627\u06cc \u0645\u062e\u062a\u0644\u0641 \u0631\u0627 \u0646\u06cc\u0632 \u0627\u0641\u0632\u0627\u06cc\u0634 \u0645\u06cc\u200c\u062f\u0647\u062f. \u0627\u062c\u0631\u0627\u06cc \u0627\u06cc\u0646 SOP \u00a0(\u0631\u0648\u0634 \u0639\u0645\u0644\u06cc\u0627\u062a\u06cc \u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 ) \u0645\u06cc\u200c\u062a\u0648\u0627\u0646\u062f \u0628\u0647 \u06a9\u0627\u0647\u0634 \u062e\u0637\u0631\u0627\u062a \u0628\u0647\u062f\u0627\u0634\u062a\u06cc \u0646\u0627\u0634\u06cc \u0627\u0632 \u0622\u0644\u0648\u062f\u06af\u06cc \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u062a\u0627\u0632\u0647 \u06a9\u0645\u06a9 \u06a9\u0631\u062f\u0647 \u0648 \u0628\u0647 \u0639\u0646\u0648\u0627\u0646 \u06cc\u06a9 \u0645\u0631\u062c\u0639 \u0639\u0644\u0645\u06cc \u0648 \u0639\u0645\u0644\u06cc \u0628\u0631\u0627\u06cc \u0622\u0632\u0645\u0627\u06cc\u0634\u06af\u0627\u0647\u200c\u0647\u0627\u06cc \u06a9\u0646\u062a\u0631\u0644 \u06a9\u06cc\u0641\u06cc\u062a\u060c \u0645\u0631\u0627\u06a9\u0632 \u062a\u062d\u0642\u06cc\u0642\u0627\u062a\u06cc \u0648 \u0628\u0631\u0646\u0627\u0645\u0647\u200c\u0647\u0627\u06cc \u0646\u0638\u0627\u0631\u062a\u06cc \u062f\u0631 \u0633\u0637\u062d \u0645\u0644\u06cc \u0648 \u0628\u06cc\u0646\u200c\u0627\u0644\u0645\u0644\u0644\u06cc \u0645\u0648\u0631\u062f \u0627\u0633\u062a\u0641\u0627\u062f\u0647 \u0642\u0631\u0627\u0631 \u06af\u06cc\u0631\u062f. \u0639\u0644\u0627\u0648\u0647 \u0628\u0631 \u0627\u06cc\u0646\u060c \u0627\u06cc\u0646 \u0631\u0648\u0634 \u067e\u0627\u06cc\u0647\u200c\u0627\u06cc \u0645\u062d\u06a9\u0645 \u0628\u0631\u0627\u06cc \u062a\u0648\u0633\u0639\u0647 \u0631\u0648\u0634\u200c\u0647\u0627\u06cc \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc \u067e\u06cc\u0634\u0631\u0641\u062a\u0647\u200c\u062a\u0631 \u0648 \u0645\u0637\u0627\u0644\u0639\u0627\u062a \u0622\u06cc\u0646\u062f\u0647 \u062f\u0631 \u0632\u0645\u06cc\u0646\u0647 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc \u0648 \u0646\u0638\u0627\u0631\u062a \u0628\u0631 \u0627\u0646\u06af\u0644\u200c\u0647\u0627 \u062f\u0631 \u0645\u0648\u0627\u062f \u063a\u0630\u0627\u06cc\u06cc \u0641\u0631\u0627\u0647\u0645 \u0645\u06cc\u200c\u06a9\u0646\u062f.<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;&#8221;]\n<table style=\"border-collapse: collapse; width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 21.2917%; text-align: left;\"><span style=\"font-size: 14pt;\">DNA Elution Solution<\/span><\/td>\n<td style=\"width: 4.7917%;\"><span style=\"font-size: 14pt;\">[3]<\/span><\/td>\n<td style=\"width: 30.9167%; text-align: left;\"><span style=\"font-size: 14pt;\">Protein Precipitation Solution<\/span><\/td>\n<td style=\"width: 4.7917%;\"><span style=\"font-size: 14pt;\">[2]<\/span><\/td>\n<td style=\"width: 32.7917%; text-align: left;\"><span style=\"font-size: 14pt;\">real-time polymerase chain reaction<\/span><\/td>\n<td style=\"width: 5.4167%;\"><span style=\"font-size: 14pt;\">[1]<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 21.2917%;\"><\/td>\n<td style=\"width: 4.7917%;\"><\/td>\n<td style=\"width: 30.9167%;\"><\/td>\n<td style=\"width: 4.7917%;\"><\/td>\n<td style=\"width: 32.7917%; text-align: left;\"><span style=\"font-size: 14pt;\">Internal Amplification Control<\/span><\/td>\n<td style=\"width: 5.4167%;\"><span style=\"font-size: 14pt;\">[4]<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_message message_box_color=&#8221;violet&#8221; icon_fontawesome=&#8221;fa fa-solid fa-book&#8221; css=&#8221;&#8221;]\n<p style=\"text-align: left;\"><span style=\"font-size: 14pt;\"><a href=\"https:\/\/www.google.com\/url?sa=t&amp;source=web&amp;rct=j&amp;opi=89978449&amp;url=https:\/\/www.fda.gov\/media\/175380\/download&amp;ved=2ahUKEwimguj9kreQAxVLgv0HHekPF2oQFnoECBgQAw&amp;usg=AOvVaw27anhqyyWhiuhLgKvOrqgK\">Cyclospora Cayetanensis Detection In Fresh Products Using Real Time PCR<\/a><\/span><\/p>\n[\/vc_message][\/vc_column][\/vc_row]\n\n\n<div class=\"kk-star-ratings kksr-auto kksr-align-right kksr-valign-bottom\"\n    data-payload='{&quot;align&quot;:&quot;right&quot;,&quot;id&quot;:&quot;30063&quot;,&quot;slug&quot;:&quot;default&quot;,&quot;valign&quot;:&quot;bottom&quot;,&quot;ignore&quot;:&quot;&quot;,&quot;reference&quot;:&quot;auto&quot;,&quot;class&quot;:&quot;&quot;,&quot;count&quot;:&quot;0&quot;,&quot;legendonly&quot;:&quot;&quot;,&quot;readonly&quot;:&quot;&quot;,&quot;score&quot;:&quot;0&quot;,&quot;starsonly&quot;:&quot;&quot;,&quot;best&quot;:&quot;5&quot;,&quot;gap&quot;:&quot;5&quot;,&quot;greet&quot;:&quot;\u0627\u0645\u062a\u06cc\u0627\u0632 \u0634\u0645\u0627 \u0628\u0647 \u0627\u06cc\u0646 \u0635\u0641\u062d\u0647&quot;,&quot;legend&quot;:&quot;0\\\/5 - (0 \u0627\u0645\u062a\u06cc\u0627\u0632)&quot;,&quot;size&quot;:&quot;18&quot;,&quot;title&quot;:&quot;\u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u062f\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u06af\u06cc\u0627\u0647\u06cc \u062a\u0627\u0632\u0647&quot;,&quot;width&quot;:&quot;0&quot;,&quot;_legend&quot;:&quot;{score}\\\/{best} - ({count} {votes})&quot;,&quot;font_factor&quot;:&quot;1.25&quot;}'>\n            \n<div class=\"kksr-stars\">\n    \n<div class=\"kksr-stars-inactive\">\n            <div class=\"kksr-star\" data-star=\"1\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"2\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"3\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"4\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" data-star=\"5\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n    <\/div>\n    \n<div class=\"kksr-stars-active\" style=\"width: 0px;\">\n            <div class=\"kksr-star\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n            <div class=\"kksr-star\" style=\"padding-left: 5px\">\n            \n\n<div class=\"kksr-icon\" style=\"width: 18px; height: 18px;\"><\/div>\n        <\/div>\n    <\/div>\n<\/div>\n                \n\n<div class=\"kksr-legend\" style=\"font-size: 14.4px;\">\n            <span class=\"kksr-muted\">\u0627\u0645\u062a\u06cc\u0627\u0632 \u0634\u0645\u0627 \u0628\u0647 \u0627\u06cc\u0646 \u0635\u0641\u062d\u0647<\/span>\n    <\/div>\n    <\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text css=&#8221;&#8221;] \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u0627 \u06a9\u0627\u06cc\u062a\u0627\u0646\u0646\u0633\u06cc\u0633 ( Cyclospora cayetanensis ) \u0627\u0646\u06af\u0644 \u062a\u06a9\u200c\u06cc\u0627\u062e\u062a\u0647\u200c\u0627\u06cc \u0627\u0632 \u06af\u0631\u0648\u0647 \u06a9\u0648\u06a9\u0633\u06cc\u062f\u06cc\u0627 \u0627\u0633\u062a \u06a9\u0647 \u0628\u0627\u0639\u062b \u0628\u06cc\u0645\u0627\u0631\u06cc \u0633\u06cc\u06a9\u0644\u0648\u0633\u067e\u0648\u0631\u06cc\u0627\u0632\u06cc\u0633 (Cyclosporiasis) \u00a0\u062f\u0631 \u0627\u0646\u0633\u0627\u0646 \u0645\u06cc\u200c\u0634\u0648\u062f. \u0627\u06cc\u0646 \u0627\u0646\u06af\u0644 \u0628\u06cc\u0634\u062a\u0631 \u0627\u0632 \u0637\u0631\u06cc\u0642 \u0645\u0635\u0631\u0641 \u0622\u0628 \u0648 \u063a\u0630\u0627\u0647\u0627\u06cc \u0622\u0644\u0648\u062f\u0647 (\u0628\u0647\u200c\u0648\u06cc\u0698\u0647 \u0633\u0628\u0632\u06cc\u062c\u0627\u062a \u0648 \u0645\u06cc\u0648\u0647\u200c\u0647\u0627\u06cc \u062e\u0627\u0645 \u0634\u0633\u062a\u0647\u200c\u0646\u0634\u062f\u0647) \u0645\u0646\u062a\u0642\u0644 \u0645\u06cc\u200c\u0634\u0648\u062f. \u0639\u0641\u0648\u0646\u062a \u0646\u0627\u0634\u06cc \u0627\u0632 \u0622\u0646 \u0628\u06cc\u0634\u062a\u0631 \u062f\u0631 \u0645\u0646\u0627\u0637\u0642 \u06af\u0631\u0645\u0633\u06cc\u0631\u06cc \u0648 \u0646\u06cc\u0645\u0647\u200c\u06af\u0631\u0645\u0633\u06cc\u0631\u06cc \u0634\u0627\u06cc\u0639 \u0627\u0633\u062a \u0648 \u0645\u0639\u0645\u0648\u0644\u0627\u064b \u0628\u0627 &hellip;<\/p>\n","protected":false},"author":3,"featured_media":30078,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16,105],"tags":[],"class_list":["post-30063","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-16","category-105"],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v25.4 (Yoast SEO v25.6) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>\u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u062f\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u06af\u06cc\u0627\u0647\u06cc \u062a\u0627\u0632\u0647 - \u0645\u062c\u0644\u0647 \u067e\u0644 \u0627\u06cc\u062f\u0647\u200c\u0622\u0644 \u067e\u0627\u0631\u0633<\/title>\n<meta name=\"description\" content=\"\u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis (\u0627\u0646\u06af\u0644 \u062a\u06a9\u200c\u06cc\u0627\u062e\u062a\u0647\u200c\u0627\u06cc \u0627\u0632 \u06af\u0631\u0648\u0647 \u06a9\u0648\u06a9\u0633\u06cc\u062f\u06cc\u0627 ) \u062f\u0631 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc\u060c \u06a9\u0646\u062a\u0631\u0644 \u0648 \u067e\u06cc\u0634\u06af\u06cc\u0631\u06cc \u0627\u0632 \u0627\u0646\u062a\u0634\u0627\u0631 \u0622\u0646 \u0627\u0647\u0645\u06cc\u062a \u0632\u06cc\u0627\u062f\u06cc \u062f\u0627\u0631\u062f.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/medpip.com\/mag\/cyclospora-cayetanensis-molecular-detection\/\" \/>\n<meta property=\"og:locale\" content=\"fa_IR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u062f\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u06af\u06cc\u0627\u0647\u06cc \u062a\u0627\u0632\u0647\" \/>\n<meta property=\"og:description\" content=\"\u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis (\u0627\u0646\u06af\u0644 \u062a\u06a9\u200c\u06cc\u0627\u062e\u062a\u0647\u200c\u0627\u06cc \u0627\u0632 \u06af\u0631\u0648\u0647 \u06a9\u0648\u06a9\u0633\u06cc\u062f\u06cc\u0627 ) \u062f\u0631 \u0634\u0646\u0627\u0633\u0627\u06cc\u06cc\u060c \u06a9\u0646\u062a\u0631\u0644 \u0648 \u067e\u06cc\u0634\u06af\u06cc\u0631\u06cc \u0627\u0632 \u0627\u0646\u062a\u0634\u0627\u0631 \u0622\u0646 \u0627\u0647\u0645\u06cc\u062a \u0632\u06cc\u0627\u062f\u06cc \u062f\u0627\u0631\u062f.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/medpip.com\/mag\/cyclospora-cayetanensis-molecular-detection\/\" \/>\n<meta property=\"og:site_name\" content=\"\u0645\u062c\u0644\u0647 \u067e\u0644 \u0627\u06cc\u062f\u0647\u200c\u0622\u0644 \u067e\u0627\u0631\u0633\" \/>\n<meta property=\"article:published_time\" content=\"2025-10-23T09:05:57+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/medpip.com\/mag\/wp-content\/uploads\/2025\/10\/molecular-detection-of-cyclospora-cayetanensis.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1280\" \/>\n\t<meta property=\"og:image:height\" content=\"720\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"sadeghzade\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@poleideal\" \/>\n<meta name=\"twitter:site\" content=\"@poleideal\" \/>\n<meta name=\"twitter:label1\" content=\"\u0646\u0648\u0634\u062a\u0647\u200c\u0634\u062f\u0647 \u0628\u062f\u0633\u062a\" \/>\n\t<meta name=\"twitter:data1\" content=\"sadeghzade\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u0632\u0645\u0627\u0646 \u062a\u0642\u0631\u06cc\u0628\u06cc \u0628\u0631\u0627\u06cc \u062e\u0648\u0627\u0646\u062f\u0646\" \/>\n\t<meta name=\"twitter:data2\" content=\"37 \u062f\u0642\u06cc\u0642\u0647\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/medpip.com\/mag\/cyclospora-cayetanensis-molecular-detection\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/medpip.com\/mag\/cyclospora-cayetanensis-molecular-detection\/\"},\"author\":{\"name\":\"sadeghzade\",\"@id\":\"https:\/\/medpip.com\/mag\/#\/schema\/person\/0951b7c35aff74d0e75cb419963f9656\"},\"headline\":\"\u062a\u0634\u062e\u06cc\u0635 \u0645\u0648\u0644\u06a9\u0648\u0644\u06cc Cyclospora cayetanensis \u062f\u0631 \u0645\u062d\u0635\u0648\u0644\u0627\u062a \u06af\u06cc\u0627\u0647\u06cc 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